Field Assessment of Agronomic Performance, Resistance to Aflatoxin, and Fumonisin Accumulation in Selected Maize Inbred Lines in Kenya
Agriculture, Forestry and Fisheries
Volume 7, Issue 4, August 2018, Pages: 94-100
Received: Nov. 6, 2018; Accepted: Nov. 29, 2018; Published: Dec. 20, 2018
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Ouko Abigael, School of Biological Sciences, University of Nairobi, Nairobi, Kenya
Okoth Sheila, School of Biological Sciences, University of Nairobi, Nairobi, Kenya
Amugune Nelson, School of Biological Sciences, University of Nairobi, Nairobi, Kenya
Vesa Joutsjoki, Natural Resources Institute Finland, Jokioinen, Finland
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Aspergillus flavus and Fusarium verticilloides cause contamination of maize (Zea mays) and concern to maize farmers because they reduce maize quantity and quality. These fungi produce mycotoxins, some of which are poisonous to both humans and animals. Over 300 mycotoxins are known but in this study aflatoxins and fumonisins produced by A. flavus and F. verticilloides, respectively, are reported due to their health concerns in Africa. Contamination of maize grain by these fungi occurs before harvest and selection of maize parental lines resistant to accumulation of aflatoxin, and fumonisin for breeding purposes is the easiest strategy to reduce consumption of maize grains contaminated by these toxins. In addition to selecting for resistant lines, breeders would prefer inbred lines with top performing agronomic traits. This study aimed at identifying possible source of resistance together with good agronomic traits among 23 maize inbred lines (13 sourced from the MAIZE Competitive Grants Initiative, International Maize and Wheat Improvement Centre and 10 from Agricultural Research Council, South Africa). The lines were planted in two blocks; Aspergillus, and Fusarium, in a randomized complete-block design for two seasons in one location in Kenya. Germination rate, days to silking, and days to anthesis were determined in each line. Inoculation of the maize ears was done at silking with three toxigenic strains of A. flavus for Aspergillus block, and F. verticilloides for Fusarium block. Aflatoxins and fumonisins concentration in the kernels was determined using Enzyme-linked immunosorbent assay (ELISA). A positive significant correlation (r = 0.9458846, P = < 9.845e-12) occurred between days to anthesis and days to silking, aflatoxins and fumonisins (r = 0.43149988, P ≤ 0.05) accumulated in the inbred lines. A negative correlation between germination and accumulated fumonisin levels (r = -0.5156961, P = 0.01178), days to pollen shed and aflatoxin (r = -0.4617732, P = 0.02654) was revealed. Apart from being good germinating lines and drought tolerant, CML 390 and CML 247 accumulated least fumonisin, and aflatoxin levels compared to the other germplasms. These two lines with consistent low aflatoxin, and fumonisin levels may, therefore be useful sources of resistance for maize breeding programs to reduce both aflatoxin and fumonisn contamination in maize. Four aflatoxin resistant lines (CB 222, CML 495 and CML 444) and one (CKL05003) fumonisin resistant line showed good agronomic traits. The lines may be suitable for breeding for resistance to aflatoxins, and fumonisins respectively in maize.
Maize Inbred Lines, Germination, Silking, Pollen Shed, Aflatoxin and Fumonisin
To cite this article
Ouko Abigael, Okoth Sheila, Amugune Nelson, Vesa Joutsjoki, Field Assessment of Agronomic Performance, Resistance to Aflatoxin, and Fumonisin Accumulation in Selected Maize Inbred Lines in Kenya, Agriculture, Forestry and Fisheries. Vol. 7, No. 4, 2018, pp. 94-100. doi: 10.11648/j.aff.20180704.11
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Okoth, S., Lindy, J. R., Ouko, A., Beukes, I., Sila, H., Mouton, M., Flett, B. C., Makumbi, D., Viljoen A. (2017). Field evaluation of resistance to aflatoxin accumulation in maize inbred lines in Kenya and South Africa. Journal of Crop Improvement, 31: 862–878.
Okoth S. (2016). Improving the evidence base on aflatoxin contamination and exposure in Africa. Agriculture and nutrition, Review. CTA working paper 16/13.
Kang’ethe E. K., Gatwiri M., Sirma A. J., Ouko e. O., Mburugu-Mosoti C. K., KitalaP. M., Ndihiu G. J., Nderitu J. G., Mungatu J. K., Hietaniemi V., Joutsjoki V., Korhonen H. J. (2017). Exposure of Kenyan population to aflatoxins in foods with special reference to Nandi and Makueni counties,” Food Quality and Safety, 1: 131–137.
MacRobert, J. F., Setimela P. S., Gethi J., Worku M. (2014). Maize. Hybrid Seed Production Manual. Mexico, D. F.: CIMMYT. ISBN: 978-607-8263-264.
Rose L., Sheila O., Beukes I., Abigael O., Mouton M., Bradley C., Makumbi D., Altus V. (2017). Determining resistance to Fusarium verticillioides and fumonisin accumulation in African maize inbred lines resistant to Aspergillus flavus and aflatoxins. Euphytica, 213: 93.
Rose L., Mouton M., Beukes I. (2016). Multi-environment evaluation of maize inbred lines for resistance to Fusarium ear rot and fumonisin. Plant Disease, 100: 10.
Okoth S., Nyongesa B., Ayugi V., Kangethe E., Korhonen H., Vesa J. (2012). Toxigenic potential of Aspergillus species occurring in maize kernels from two agro- ecological zones. Toxins, 4: 491-1007.
Bii, F., Wanyoike W., Nyande A. B., Gituru R. W., Bii C. (2012). Fumonisin contamination of maize (Zea mays) in aflatoxin hot zones in Eastern Province of Kenya. African Journal of Health Sciences 20: 28-36.
Probst, C., Njapau H., Cotty P. J. (2007). Outbreak of an acute aflatoxicosis in Kenya in 2004: Identification of the causal agent. Applied and Environmental Microbiology, 73: 2762–64.
Suleiman R. A. and Rosentrater K. A. (2015). Current maize production, post-harvest losses and the risk of mycotoxins contamination in Tanzania. Agriculture, 1-128.
Mwalwayo D. and Thole B. (2016). Prevalence of aflatoxin and fumonisins (B1 +B2) in maize consumed in rural Malawi. 3(173-179).
KBS (Kenya Bureau of Standards), 2013. The Benchmark. The official magazine of the Kenya Bureau of Standards, Issue 19, pp 39.
Guo B., Xiangyun J., Xinzhi N., Jake C., Hong L., Hammed K., Robert D., Brian T. (2017). Evaluation of maize inbred lines for resistance to pre-harvest aflatoxin and fumonisin accumulation in the field. Crop Journal, 259-264.
Obura A. (2013) Aflatoxins: Finding solutions for improved food safety. Aflatoxicosis: Evidence from Kenya. Agriculture for Nutrition and health.
Wagacha J. M. and J. W. Muthomi (2008). Mycotoxin problem in Africa: Current status, implications to food safety and health and possible management strategies. International Journal of Food Microbiology, 124: 1-12.
Kibe N. (2015). Thesis. Occurrence of mycotoxigenic fungi in maize from food commodity markets in Kenya. Ghent university.
Bernahu T. E., Beyeni Y., Biswanata D., Mugo S., Olsen M., Oikeh S., Juma C., Labuschagne M., Prasanna B. M. (2017). Combining ability and test cross performance of drought tolerant maize inbred lines under stress and non-stress environments in Kenya. Plant breeding 136,197-205.
Finch-Savage W. E. and Bassel G. W. (2016). Seed vigour and crop establishment: extending performance beyond adaptation. Journal of experimental Botany, 67: 567-591.
Harmeet S., Rupinder K. J., Kang J. S., Sandhu S. S., Harrajdeep K., Kamaljit G. (2015). Seed priming techniques in field crops – Agriculture, Review, 36 (4): 251-264.
Ngugi K., Jerono C., Muchira C. and Chemining’wa G. (2013). Anthesis to silking interval usefulness in developing drought tolerant maize. Journal of renewable agriculture, 1(5): 84-90.
Booth C (1971) The genus Fusarium. Commonwealth Mycological Institute, Kew, p 237.
Small M., Flett C., Marasas W., McLeod A., Stander M., Viljoen A. (2012) Resistance in maize inbred lines to Fusarium verticillioides and fumonisin accumulation in South Africa. Plant Diseases, 96: 881–888.
Zummo, N., and Scott G. E. (1989). Evaluation of field inoculation techniques for screening maize genotypes against kernel infection by Aspergillus flavus in Mississippi. Plant Diseases, 73: 313–16.
Felipe de Mendiburu (2017). agricolae: Statistical Procedures for Agricultural Research. R package version 1.2-8. http://CRAN.
R Core Team (2015). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL
Bushra M. and Jones D. A. (1983). The genetics of maize (Zea mays L.) growing at low temperatures. Euphytica, 32 (2) 535-542.
Williams, W. P., Krakowsky M. D., Scully B. T., Brown R. L., Menkir A., Warburton M. L., Windham G. L. (2014). Identifying and developing maize germplasm with resistance to accumulation of aflatoxins. World Mycotoxin, 8 (2): 193–209.
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