Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl

Srinivas Devaraju; K. Jaganmohan Reddy

doi:doi:10.11648/j.aff.20130201.14

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Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl

Srinivas Devaraju, K. Jaganmohan Reddy

Published in Agriculture, Forestry and Fisheries (Volume 2, Issue 1)

Received: Accepted: Published: 20 February 2013

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Abstract

Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.

Published in	Agriculture, Forestry and Fisheries (Volume 2, Issue 1)
DOI	10.11648/j.aff.20130201.14
Page(s)	33-37
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Leaf Explants, Sapindus Emarginatus, Somatic Embryogenesis

References

[1]	Ammirato PV (1983).The regulation of somatic embryos development in plant cell cultures, suspension cultures tech-nique and hormone requirements. Bio. Technol. 1: 68-74.
[2]	Attree SM and Fowke LC (1993). Embryogeny of gymnos-perms advances in synthetic seed technology of Conifers. Plant Cell Tissue Org. Cult. 35: 1-35.
[3]	Binzel ML, Sanhla AN, Joshi S and Sankhla D (1996). In vitro regeneration in chili pepper (Capsicum annuum L.) from half-seed explant. Plant Growth Regul. 20: 287-293.
[4]	Gill R and Saxena PK (1992). Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogeae) promotive role of thidiazuron. Can. J. Plant Sci. 70:1186 –1192.
[5]	Finer JJ (1988). Apical proliferation of embryogenic tissue of soyabean (Glycine max L.) Plant Cell Reports. 7: 238-241.
[6]	Finer JJ (1994). Plant regeneration via embryo genic susper-sion cultures. In: Plant Cell Cultures. A practical approach. Dixon RA and Gonzales RA (eds) Oxford University Press, Oxford, pp. 67 – 102.
[7]	Fitch MM (1990). High frequency Somatic embryogenesis and plant regeneration from papaya hypocotyl callus. Plant Cell Tiss.Org. Cult. 32: 205-212.
[8]	Lazzeri PA, Hildebrand DF Collins GB (1985). A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol. Biol. Rep. 3:160-167.
[9]	Litz RE and Gray (1995). In vitro Somatic embryogenesis from Mangifera indica L. Callus. Sci. Hort. 22: 233-240.
[10]	Maheshwaran G and Williams EG (1986). Primary and Sec-ondary Direct Somatic Embryogenesis from Immature Zy-gotic Embryos of Brassica campestris. J. Plant Physiol. 124: 455-463.
[11]	Malik KA and Saxena PK (1992). Somatic embryogenesis and shoot regeneration from intact seedlings of Phaseolus acutifolius, P. aureus, P. coccineus and P. wrightii. Plant Cell Rep. 11: 163–168.
[12]	Mallikarjuna N, Reena MJT, Sastri DC and Moss JP. (1996). Somatic embryogenesis in Pigeonpea Cajanus cajan L. In-dian J. Exp. Biol. 34: 282-284.
[13]	Murthy BNS, Murch SJ and Saxena PK (1998). Thidiazuron: A potent Regulator of in vitro plant morphogenesis in vitro cell development. Biol. Plant. 34: 267–275.
[14]	Ranch JP, Oglesby L, Zielinski AC, (1985). Plant regenera-tion from embryo derived tissue cultures of soybean. In Vitro Cell Dev. Biol. 21: 653-658.
[15]	Reddy P and Reddy S (1983) Scope for Production rise. The Hindu Survey of Indian Agriculture.
[16]	Reinert J (1958) Uber die Kontrolle der morphogenese und die Inducan von Adventive embronen an Gewebekulturen aus Karotten. Planta 53: 318 – 333.
[17]	Reinert J (1959). Uber die kontrolle der morphogenese and die inductionVon Advientive‚ embryonen & Gewebekuluren aus kartten. Planta 58: 318-333.
[18]	Saxena C, Palai SK, Samantaray S, Rout GR and Das P (1997). Plant regeneration from callus cultures of Psoralea corylifolia Linn. Plant Growth Regul. 22: 13-17.
[19]	Shoemaker RC, Amberger LA and Palmer RG (1991). Ef-fects of 2, 4-Dichlorophenoxy acetic acid on somatic em-bryogenesis and heritable variation in soybean (Glycine max (L.) Merr.) in vitro. Cell Dev. Biol. 27: 84-88.
[20]	Steward FC, Mcpes MG and Meass K (1958).Growth & organized development of cultured cell organization in cul-tured grown from freely suspended cells. Amer. J. Bot. 445: 705-708.
[21]	Parrott WA, Hoffmann LM, Hildebrand DR, Williams EG and Collins GB (1989). Recovery of primary transformaonts of soybean. Plant Cell Rep. 7: 615- 617.
[22]	Tomar UK. and Gupta SC (1986). Somatic embryogenesis and organogenesis in callus cultures of a free legume Albizzia richardiana King. Plant Cell Rep. 7: 70-73.

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APA Style

Srinivas Devaraju, K. Jaganmohan Reddy. (2013). Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agriculture, Forestry and Fisheries, 2(1), 33-37. https://doi.org/10.11648/j.aff.20130201.14

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ACS Style

Srinivas Devaraju; K. Jaganmohan Reddy. Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agric. For. Fish. 2013, 2(1), 33-37. doi: 10.11648/j.aff.20130201.14

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AMA Style

Srinivas Devaraju, K. Jaganmohan Reddy. Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agric For Fish. 2013;2(1):33-37. doi: 10.11648/j.aff.20130201.14

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@article{10.11648/j.aff.20130201.14,
  author = {Srinivas Devaraju and K. Jaganmohan Reddy},
  title = {Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {2},
  number = {1},
  pages = {33-37},
  doi = {10.11648/j.aff.20130201.14},
  url = {https://doi.org/10.11648/j.aff.20130201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20130201.14},
  abstract = {Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.},
 year = {2013}
}

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TY  - JOUR
T1  - Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl
AU  - Srinivas Devaraju
AU  - K. Jaganmohan Reddy
Y1  - 2013/02/20
PY  - 2013
N1  - https://doi.org/10.11648/j.aff.20130201.14
DO  - 10.11648/j.aff.20130201.14
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 33
EP  - 37
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20130201.14
AB  - Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.
VL  - 2
IS  - 1
ER  -

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Author Information

Srinivas Devaraju

Dept. of Botany, Telangana University, Nizamabad, Andhra Pradesh, India
K. Jaganmohan Reddy

Dept. of Botany, Kakatiya University, Warangal, Andhra Pradesh, India

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APA Style

Srinivas Devaraju, K. Jaganmohan Reddy. (2013). Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agriculture, Forestry and Fisheries, 2(1), 33-37. https://doi.org/10.11648/j.aff.20130201.14

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ACS Style

Srinivas Devaraju; K. Jaganmohan Reddy. Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agric. For. Fish. 2013, 2(1), 33-37. doi: 10.11648/j.aff.20130201.14

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AMA Style

Srinivas Devaraju, K. Jaganmohan Reddy. Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl. Agric For Fish. 2013;2(1):33-37. doi: 10.11648/j.aff.20130201.14

Copy | Download

@article{10.11648/j.aff.20130201.14,
  author = {Srinivas Devaraju and K. Jaganmohan Reddy},
  title = {Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {2},
  number = {1},
  pages = {33-37},
  doi = {10.11648/j.aff.20130201.14},
  url = {https://doi.org/10.11648/j.aff.20130201.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20130201.14},
  abstract = {Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.},
 year = {2013}
}

Copy | Download

TY  - JOUR
T1  - Study of Somatic Embryogenesis in Leaf Explants of Sapindus Emarginatus Vahl
AU  - Srinivas Devaraju
AU  - K. Jaganmohan Reddy
Y1  - 2013/02/20
PY  - 2013
N1  - https://doi.org/10.11648/j.aff.20130201.14
DO  - 10.11648/j.aff.20130201.14
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 33
EP  - 37
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20130201.14
AB  - Plant regeneration through Somatic embryogenesis has several advantages over other routes to in vitro plant production and appears that most promising area of research for large scale production and rapid plant propagation. Sapindus emarginatus vahl commonly known as soap nut tree wherein the nut shell contains saponin, which acts like soap when comes in contact with water. It has very high economic and medicinal values in saponin industry and is also being used for important medicinal properties. The present study is aimed on somatic embryogenesis from leaf explants of Sapindus emarginatus Vahl employing auxin such as 2, 4-D along with cytokinin BAP/Kn. The present protocol is the first ever reported of its kind and is unique in itself as the latex poses a threat for tissue culture.
VL  - 2
IS  - 1
ER  -

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