Study on Preparation of Immobilized Alkaline Protease and Its Performance
Modern Chemistry
Volume 5, Issue 3, June 2017, Pages: 35-42
Received: May 10, 2017; Published: May 10, 2017
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Authors
Yiming Li, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Fei Xue, College of Environmental Science and Engineering, Donghua University, Shanghai, China
Dengxin Li, College of Environmental Science and Engineering, Donghua University, Shanghai, China
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Abstract
The nano-Fe3O4 particles were prepared by hydrothermal method. Cellulose-based magnetic Fe3O4 was prepared by adding cellulose solution dissolved in sodium hydroxide / thiourea / urea system. The basic protease was used as the catalyst and glutaraldehyde was used as the crosslinking agent, The production of magnetic recyclable immobilized enzyme can greatly reduce the cost of hydrolysis of sludge. The aldehyde groups in the glutaraldehyde and the amino groups in the protein can be stably crosslinked by coupling. The immobilization effect of nanometer Fe3O4 was reflected by investigating the activity of immobilized enzyme and immobilization rate of enzyme under different conditions.
Keywords
Nano Fe3O4, Glutaraldehyde, Immobilized Enzyme, Alkaline Protease
To cite this article
Yiming Li, Fei Xue, Dengxin Li, Study on Preparation of Immobilized Alkaline Protease and Its Performance, Modern Chemistry. Vol. 5, No. 3, 2017, pp. 35-42. doi: 10.11648/j.mc.20170503.11
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