FGF-2, MMP-8 and Integrin α2β1 Expression in Periodontal ligament Remodelling Tension Area with Nanopowder Stichopus hermanii Application to Prevent Orthodontic Relapsing
International Journal of Materials Science and Applications
Volume 6, Issue 6, November 2017, Pages: 284-289
Received: Oct. 19, 2017; Accepted: Oct. 23, 2017; Published: Nov. 16, 2017
Views 2205      Downloads 107
Noengki Prameswari, Orthodontics Department, Dentistry Faculty, Hang Tuah University, Surabaya, Indonesia
Puguh Bayu Prabowo, Dental Material Department, Dentistry Faculty, Hang Tuah University, Surabaya, Indonesia
Article Tools
Follow on us
Background: Orthodontic relapse tendency occurs 33–90 per cent after at least 10 years post-treatment. Orthodontic Relapsing is the return, following orthodontic correction, at the end of treatment. Relapse, a return to the original teeth position, caused by periodontal, occlusal, soft tissue factor and growth. Nanopowder Stichopus hermanii have various active ingredient such as protein, collagen, flavonoid, chondroitin sulphate, cell growth factor, EPA DHA, that might have role to FGF-2, MMP-8, Integrin α2β1 in periodontal ligament remodelling tension area in reducing orthodontic relapsing. Objectives: The aim of this study is to investigate application of Nanopowder Stichopus hermanii to prevent relaps orthodontic by periodontal ligament remodelling tension area through FGF-2, MMP-8, and Integrin α2β1 parameter. Material and Method: The experiment was held by Post Test Only Group design. Twenty four male Cavia Cobaya were divided into three groups. K(-) group as negative control group (without treatment), K(+) group as positive control group which were applied with relaps orthodontic forces, and the other groups P, were applied with relaps orthodontic forces and nanopowder Stichopus hermanii 3%. After treatment the cavia cobaya were sacrificed. FGF-2, MMP-8, and Integrin α2β1 expression is examined with immunohistochemistry. Results: Application nanopowder Stichopus hermanii can reduce relaps until 30%. This study showed FGF-2 expresssion are 13,9±2,6; 6,5±1,4; 21±4,6; MMP-8 expression means and SD in K(-), K(+), P are 3.75±1.49; 14.88±2.64; 9.13±1 and Integrin α2β1 expression are 5.25±1.28; 12.88±1.36; 16.50±2.33. There was significantly differences in group P compare to K(-) and K(+). Conclusion: Application of Nanopowder Stichopus hermanii have role in periodontal ligament remodeling tension area through increasing FGF-2, decreasing MMP-8, increasing Integrin α2β1 and Collagen type 1 parameter to prevent relaps orthodontic until 30%.
Nanopowder Stichopus hermanii, FGF-2, MMP-8, Integrin α2β1, Relaps Orthodontic
To cite this article
Noengki Prameswari, Puguh Bayu Prabowo, FGF-2, MMP-8 and Integrin α2β1 Expression in Periodontal ligament Remodelling Tension Area with Nanopowder Stichopus hermanii Application to Prevent Orthodontic Relapsing, International Journal of Materials Science and Applications. Vol. 6, No. 6, 2017, pp. 284-289. doi: 10.11648/j.ijmsa.20170606.13
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Franzen, TJ, Brudvik P, Radunovic VV. 2011. Periodontal Tissue Reaction during Orthodontic Relapse in Rat Molars. European Journal of Orthodontics. Vol 35(2). Pp 152-9. October 2011
English J. Orthodontic Review. 1st edition. Mosby Elsevier. Missouri. 2009
Young L, Binderman I, Yaffe A, Beni L, Vardimon AD. Fiberotomy Enhances Orthodontic Tooth Movement and diminishes Relapse in a Rat Model. Orthod Craniofac Res vol 16(3): pp 161-8. 2013
Sendih, Skolastika, Gunawan, Keajaiban Teripang Penyembuh Mujarab Dari Laut. Jakarta: Agro Media Pustaka. 2006
Sze CP. Fisheries, Trade and Utilization of Sea Cucumbers in Malaysia. WorldFish Center, Penang, Malaysia. 2000
Bordbar S, Anwar F, Saari N. High-value Components and Bioactives from Sea Cucumber for Functional Foods- A Review. Marine Drugs Vol 9. pp 1761-1805. 2011
Tehranifard A, Oryan S, Vosoghi G. Introducing a Holothorian sea cucumber species Stichopus hermanni form Kish island in the Persian Gulf in IRAN. International Conference on Environment and Industrial Innovation. IPCBEE vol. 12. 2011
Revianti S, Soetjipto, Rahayu RP, Parisihni K. Protective Role of Stichopus hermanii ethanol extract supplementation to oxidative stress and oral hyperkeratosis in smoking Exposed Rats. International Journal of Chemtech Research. 9.(5). 408-407. 2016
Prameswari N. Soetjipto, Rahayu RP. Osteogenesis at Tension Site by Stichopus hermanii Application as Relapse Orthodontic Prevention. International Journal of ChemTech Research. 9.(6). 686-693. 2016
Zohdi RM, Zakaria ZA, Yusof N, Mustapha NM, Abdullah MN. Sea Cucumber (Stichopus hermanii) based Hydrogel to Treat Burn Wounds in Rats. Journal Biomed Mater Res vol 98(1): 30-7. 2011
Shahrulazua A, Samsudin AR, Iskandar MA, Amran. 2013. The In In-Vitro Effects of Sea Cucumber (Stichopus sp1) Extract on Human Osteoblast Cell Line. Malaysian Orthopaedic Journal 2013 Vol 7 (1). 2013
Proffit WR, Fields HW. Sarver DM. Contemporary Orthodontic. 4th edition. Mosby Elsevier. Missouri. 2007
Yoshida Y, Sasaki T, Yokoya K, Hiraide T, Shibasaki Y. Cellular Roles in Relapse Processes of Experimentally-moved Rat Molars. Electron Microsc 1998(48): 147-157. 1999
Krishnan V., Davidovitch Z. Cellular, molecular, and tissue-level reactions to orthodontic force. American Journal of Orthodontics and Dentofacial Orthopedics. Vol 129(4): 469.e1–469.e32. 2006
Feller L, Khammisa R, Schechter I, Thomadakis G, Fourie J, Lemmer J. Biological Events in Periodontal Ligament and Alveolar Bone Associated with Application of Orthodontic Forces. Scientific Word Journal. Vol 2015. 876509. 2015
Henneman S., Von Den Hoff J. W., Maltha J. C. Mechanobiology of tooth movement. European Journal of Orthodontics. Vol 30(3): 299–306. 2008
D'Apuzzo F., Cappabianca S., Ciavarella D., Monsurrò A., Silvestrini-Biavati A., Perillo L. Biomarkers of periodontal tissue remodeling during orthodontic tooth movement in mice and men: overview and clinical relevance. The Scientific World Journal. 2013; 2013: 8
Galicka A, Nazaruk J. Stimulation of Collagen Biosynthesis by Flavonoid Glycosides in Skin Fibroblasts of Osteogenesis Imperfecta Type 1 and the Potential Mechanism of their action. International Journal of Molecular Medicine 20: 889-895. 2007
Shimabukuro Y, Ichikawa T, Terashima Y, Oohara H, Kajikawa T, Kobayashi R, Terashima H, Takedachi M, Terakura M, Hashikawa T, Yamada S, Murakami S. Basic Fibroblast Growth Factor Regulates Expression of Heparan Sulfate in Human Periodontal Ligament Cells. Matrix Biol 27(3): 232-41. 2008
Terashima Y, Shimabukuro Y, Terashima H, Ozasa M, Terakura M, IkezawaK, Hashikawa T, Takedachi M, Oohara H, Yamada S, Murakami S. Fibroblast Growth Factor-2 regulates expression of osteopontin in periodontal ligament cells. J Cell Physiol 216(3): 640-50. 2008
Ishijima M, Rittling SR, Yamashita T, Tsuji K, Kurosawa H, Nifuji A, Denhardt DT, Noda M. Enhancement of Osteoclastic Bone Resorption and Suppression of Osteoblastic Bone Formation in Response to Reduced Mechanical Stress Do Not Occur in the Absence of Osteopontin. J. Exp. Med. Vol 193 (3): 399-404. 2001
Shetty S, Mogra S, Shetty S. The Effect of the Short Term Administration of a Biphosphonate upon Dental Relapse. J Ind Orthod Soc vol 39: 198-203. 2006
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186