FGF-2, MMP-8 and Integrin α2β1 Expression in Periodontal ligament Remodelling Tension Area with Nanopowder Stichopus hermanii Application to Prevent Orthodontic Relapsing
International Journal of Materials Science and Applications
Volume 6, Issue 6, November 2017, Pages: 284-289
Received: Oct. 19, 2017; Accepted: Oct. 23, 2017; Published: Nov. 16, 2017
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Authors
Noengki Prameswari, Orthodontics Department, Dentistry Faculty, Hang Tuah University, Surabaya, Indonesia
Puguh Bayu Prabowo, Dental Material Department, Dentistry Faculty, Hang Tuah University, Surabaya, Indonesia
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Abstract
Background: Orthodontic relapse tendency occurs 33–90 per cent after at least 10 years post-treatment. Orthodontic Relapsing is the return, following orthodontic correction, at the end of treatment. Relapse, a return to the original teeth position, caused by periodontal, occlusal, soft tissue factor and growth. Nanopowder Stichopus hermanii have various active ingredient such as protein, collagen, flavonoid, chondroitin sulphate, cell growth factor, EPA DHA, that might have role to FGF-2, MMP-8, Integrin α2β1 in periodontal ligament remodelling tension area in reducing orthodontic relapsing. Objectives: The aim of this study is to investigate application of Nanopowder Stichopus hermanii to prevent relaps orthodontic by periodontal ligament remodelling tension area through FGF-2, MMP-8, and Integrin α2β1 parameter. Material and Method: The experiment was held by Post Test Only Group design. Twenty four male Cavia Cobaya were divided into three groups. K(-) group as negative control group (without treatment), K(+) group as positive control group which were applied with relaps orthodontic forces, and the other groups P, were applied with relaps orthodontic forces and nanopowder Stichopus hermanii 3%. After treatment the cavia cobaya were sacrificed. FGF-2, MMP-8, and Integrin α2β1 expression is examined with immunohistochemistry. Results: Application nanopowder Stichopus hermanii can reduce relaps until 30%. This study showed FGF-2 expresssion are 13,9±2,6; 6,5±1,4; 21±4,6; MMP-8 expression means and SD in K(-), K(+), P are 3.75±1.49; 14.88±2.64; 9.13±1 and Integrin α2β1 expression are 5.25±1.28; 12.88±1.36; 16.50±2.33. There was significantly differences in group P compare to K(-) and K(+). Conclusion: Application of Nanopowder Stichopus hermanii have role in periodontal ligament remodeling tension area through increasing FGF-2, decreasing MMP-8, increasing Integrin α2β1 and Collagen type 1 parameter to prevent relaps orthodontic until 30%.
Keywords
Nanopowder Stichopus hermanii, FGF-2, MMP-8, Integrin α2β1, Relaps Orthodontic
To cite this article
Noengki Prameswari, Puguh Bayu Prabowo, FGF-2, MMP-8 and Integrin α2β1 Expression in Periodontal ligament Remodelling Tension Area with Nanopowder Stichopus hermanii Application to Prevent Orthodontic Relapsing, International Journal of Materials Science and Applications. Vol. 6, No. 6, 2017, pp. 284-289. doi: 10.11648/j.ijmsa.20170606.13
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Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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