Effect of Silicon and Calcium on Human Dental Pulp Cell Cultures
International Journal of Materials Science and Applications
Volume 6, Issue 6, November 2017, Pages: 290-296
Received: Sep. 10, 2017; Accepted: Sep. 21, 2017; Published: Dec. 4, 2017
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Jomana Alsenan, Department of Restorative Sciences & Biomaterials, Goldman School of Dental Medicine, Boston University, Boston, USA
Laisheng Chou, Department of Restorative Sciences & Biomaterials, Goldman School of Dental Medicine, Boston University, Boston, USA
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This study was designed to investigate the odontogenic effects of Silicon, Calcium and Phosphorous on human dental pulp cells. Human dental pulp cells derived from extracted pristine teeth were cultured in growth media with supplements of Si 25ppm, Si 25ppm+Ca 8.3ppm, Si 25ppm+Ca 8.3ppm+P 4.16ppm, Si 50ppm, Si 50ppm+Ca 16.7ppm, Si 50ppm+Ca 16.7ppm+P 8.3ppm and media without additional supplement as control, for the time intervals of 16 hours, 7, 12, and 21 days. Cell proliferation rates were measured by the optical density of crystal violet dye stained cells. ALP activity was measured by fluorometric assay. Expression of Dentin Sialoprotein (DSP) was measured by ELISA. Mineralization of cultures was measured by Alizarin Red staining. The data were presented as the mean of triplicates and normalized on a per million cell basis. Statistical analysis was conducted using ANOVA and Tukey HSD post-hoc tests. Culture with 50ppm supplemental Si at day 21 yield significantly higher levels of ALP activity, DSP expression and mineralization (P<0.05) compared to the control group and other supplemented groups. Cultures with Si 25ppm+Ca 8.3ppm supplemental and Si 50ppm+Ca 16.7ppm supplemental displayed significantly higher cell proliferation rates compared to the control group at day 12 (P<0.05) and at day 21 (P<0.05). Supplemental silicon in concentration of 50 ppm could significantly induce differentiation and mineralization of normal human dental pulp cells. Calcium has a synergetic effect in up-regulating the proliferation rates. This is the first report to demonstrate the silicon- and calcium-induced mineralized tissue formation of human dental pulp cell cultures, leading to the potential development and clinical application of a future novel dental pulp capping material.
Pulp Capping, Odontogenesis, Silicon, Calcium
To cite this article
Jomana Alsenan, Laisheng Chou, Effect of Silicon and Calcium on Human Dental Pulp Cell Cultures, International Journal of Materials Science and Applications. Vol. 6, No. 6, 2017, pp. 290-296. doi: 10.11648/j.ijmsa.20170606.14
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