Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering
International Journal of Materials Science and Applications
Volume 7, Issue 2, March 2018, Pages: 62-68
Received: Feb. 23, 2018;
Accepted: Mar. 9, 2018;
Published: Apr. 2, 2018
Views 1247 Downloads 53
Nisat Tamanna Nipu, Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka, Bangladesh
Farzana Khan Rony, Institute of Glass and Ceramic Research and Testing, Bangladesh Council of Scientific and Industrial Research, Dhaka, Bangladesh
Asaduz Zaman, Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka, Bangladesh
In this research work, gelatin-carboxymethylchitosan (CMC) based biodegradable composites films were prepared by solution casting method. Chitosan from waste prawn shell was the basic raw materials of CMC synthesis. Five sets of CMC-gelatin composites (5-25 wt% CMC) along-with pure gelatin were prepared in solution casting method. Incorporation of CMC into gelatin significantly altered some of the properties. The CMC and gelatin-CMC composites formation was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). Surface morphology of the films was investigated by Scanning Electron Microscopy (SEM) and SEM micrograph revealed that composites were porous and CMC was homogenously dispersed into gelatin. The porous surface of the composites is one of the criterions for new cells growth. Thermal stability of composites were investigated by thermogravimetric analysis (TGA) and composites more thermal stable (less weight loss) than pure gelatin. Antimicrobial and cytotoxicity tests found all composites were performed microbial safe and no cytotoxic effect. The physico-chemical analyses and others analyses of scaffolds revealed for their application as a wound dressing material or artificial skin.
Nisat Tamanna Nipu,
Farzana Khan Rony,
Preparation and Characterization of Porous Scaffold Composite Films by Blending Carboxymethyl Chitosan and Gelatin for Tissue Engineering, International Journal of Materials Science and Applications.
Vol. 7, No. 2,
2018, pp. 62-68.
Madihally, S. V. and H. W. Matthew, Porous chitosan scaffolds for tissue engineering. Biomaterials, 1999. 20(12): p. 1133-1142.
Agarwal, T., et al., Gelatin/Carboxymethyl chitosan based scaffolds for dermal tissue engineering applications. International journal of biological macromolecules, 2016. 93: p. 1499-1506.
Maji, S., et al., Development of gelatin/carboxymethyl chitosan/nano-hydroxyapatite composite 3D macroporous scaffold for bone tissue engineering applications. Carbohydrate Polymers, 2018.
Chapekar, M. S., Tissue engineering: challenges and opportunities. Journal of Biomedical Materials Research Part A, 2000. 53(6): p. 617-620.
Yin, Y., et al., Preparation and characterization of macroporous chitosan–gelatin/β‐tricalcium phosphate composite scaffolds for bone tissue engineering. Journal of Biomedical Materials Research Part A, 2003. 67(3): p. 844-855.
Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering. Carbon, 2017. 116: p. 325-337.
Baek, J., et al., Meniscal tissue engineering using aligned collagen fibrous scaffolds: comparison of different human cell sources. Tissue Engineering Part A, 2018. 24(1-2): p. 81-93.
Kim, S., et al., Chitosan/gelatin–based films crosslinked by proanthocyanidin. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005. 75(2): p. 442-450.
Saravanan, S., et al., Scaffolds containing chitosan, gelatin and graphene oxide for bone tissue regeneration in vitro and in vivo. International journal of biological macromolecules, 2017. 104: p. 1975-1985.
Ji, C. and J. Shi, Thermal-crosslinked porous chitosan scaffolds for soft tissue engineering applications. Materials Science and Engineering: C, 2013. 33(7): p. 3780-3785.
Kong, L., et al., A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering. European Polymer Journal, 2006. 42(12): p. 3171-3179.
Wang, G., et al., Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats. Biotechnology Letters, 2010. 32(1): p. 59.
Bukzem, A. L., et al., Optimization of carboxymethyl chitosan synthesis using response surface methodology and desirability function. International journal of biological macromolecules, 2016. 85: p. 615-624.
Hjerde, R. J. N., et al., Chemical composition of O-(carboxymethyl)-chitins in relation to lysozyme degradation rates. Carbohydrate Polymers, 1997. 34(3): p. 131-139.
Fiamingo, A. and S. P. Campana-Filho, Structure, morphology and properties of genipin-crosslinked carboxymethylchitosan porous membranes. Carbohydrate Polymers, 2016. 143: p. 155-163.
Chen, X.-G. and H.-J. Park, Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions. Carbohydrate Polymers, 2003. 53(4): p. 355-359.
Thi, T. T. H., et al., Oxidized cyclodextrin-functionalized injectable gelatin hydrogels as a new platform for tissue-adhesive hydrophobic drug delivery. RSC Advances, 2017. 7(54): p. 34053-34062.
Jorgensen, J. H. and J. D. Turnidge, Susceptibility Test Methods: Dilution and Disk Diffusion Methods*. 2015.
Sheikholeslami, Z. S., et al., Exploring the effect of formulation parameters on the particle size of carboxymethyl chitosan nanoparticles prepared via reverse micellar crosslinking. Journal of microencapsulation, 2017. 34(3): p. 270-279.
Wongkom, L. and A. Jimtaisong, Novel biocomposite of carboxymethyl chitosan and pineapple peel carboxymethylcellulose as sunscreen carrier. International journal of biological macromolecules, 2017. 95: p. 873-880.