Isotopic Abundance Ratio Analysis of Biofield Energy Treated Indole Using Gas Chromatography-Mass Spectrometry
Science Journal of Chemistry
Volume 4, Issue 4, August 2016, Pages: 41-48
Received: May 10, 2016;
Accepted: Aug. 2, 2016;
Published: Aug. 21, 2016
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Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Kalyan Kumar Sethi, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
The objective of the current experiment was to evaluate the effect of biofield energy treatment on the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N) in indole using the gas chromatography-mass spectrometry (GC-MS). The sample of organic compound indole was divided into two parts - one part was designated as a control sample (untreated), and another part was considered as biofield energy treated sample, which was subjected to Mr. Trivedi’s biofield energy treatment (The Trivedi Effect®). The biofield energy treated indole sample was analyzed at different time intervals and were symbolized as T1, T2, T3, and T4 to understand the effect of the biofield energy on isotopic abundance ratio with respect to the time. From the GC-MS spectra, the presence of the molecular ion peak C8H7N+ (m/z 117) along with major fragmented peaks C7H6+ (m/z 90), C7H5+ (m/z 89), C5H3+ (m/z 63), C4H2+ (m/z 50), C3H3+ (m/z 39), and C2H4 (m/z 28) were observed in both control and biofield treated samples. Only, the relative peak intensities of the fragmented ions in the biofield treated indole was notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis of indole using GC-MS revealed that the isotopic abundance ratio of PM+1/PM in the biofield energy treated indole at T1 and T2 was significantly decreased by 44.28 and 28.18% as compared to the control sample. On the contrary, the isotopic abundance ratio of PM+1/PM in the biofield energy treated sample at T3 and T4, was significantly increased by 41.22 and 180.88%, respectively as compared to the control sample. Overall, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N) was significantly altered in the biofield energy treated indole as compared to the control with respect to the time. The biofield treated indole with the altered isotopic abundance ratio might have altered the physicochemical properties and rate of reaction. This biofield energy treated indole might be more useful as a chemical intermediate in the production of pharmaceuticals, chemicals, plastics, dyes, and perfumes.
Mahendra Kumar Trivedi,
Kalyan Kumar Sethi,
Isotopic Abundance Ratio Analysis of Biofield Energy Treated Indole Using Gas Chromatography-Mass Spectrometry, Science Journal of Chemistry.
Vol. 4, No. 4,
2016, pp. 41-48.
Radwanski ER, Last RL (1995) Tryptophan biosynthesis and metabolism: Biochemical and molecular genetics. Plant Cell 7: 921-934.
Berger M, Gray JA, Roth BL (2009) The expanded biology of serotonin. Annu Rev Med 60: 355-366.
Van Order RB, Lindwall HG (1942) Indole. Chem Rev 30: 69-96.
Kaushik NK, Kaushik N, Attri P, Kumar N, Kim CH, Verma AK, Choi EH (2013) Biomedical importance of indoles. Molecules 18: 6620-6662.
Barden TC (2011) Indoles: Industrial, agricultural and over-the-counter uses. Top Heterocycl Chem 26: 31-46.
Kim J, Hong H, Heo A, Park W (2013) Indole toxicity involves the inhibition of adenosine triphosphate production and protein folding in Pseudomonas putida. FEMS Microbiol Lett 343: 89-99.
Dzhioev FK (1974) Study of the carcinogenic action of tryptophan and certain of its metabolites. Vopr Onkol 20: 75-81.
A Framework to Guide Selection of Chemical Alternatives (2014) The National Academies Press. Chapter 5: Physicochemical Properties and Environmental Fate.
Winderl C, Penning H, von Netzer F, Meckenstock RU, Lueders T (2010) DNA-SIP identifies sulfate-reducing Clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment. The ISME Journal 4: 1314-1325.
Muccio Z, Jackson GP (2009) Isotope ratio mass spectrometry. Analyst 134: 213-222.
Ben-David M, Flaherty EA (2012) Stable isotopes in mammalian research: A beginner's guide. J Mammal 93: 312-328.
Scott, KM, Fox, G, Girguis PR (2011) Measuring isotope fractionation by autotrophic microorganisms and enzymes. Methods Enzymol 494: 281-299.
Morgan JLL, Skulan JL, Gordon GW, Romaniello SJ, Smith SM, Anbar AD (2012) Rapidly assessing changes in bone mineral balance using natural stable calcium isotopes. Proc Natl Acad Sci USA 109: 9989-9994.
Hayes JM (2004) An introduction to isotopic calculations. Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA.
Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Evaluation of isotopic abundance ratio of naphthalene derivatives after biofield energy treatment using gas chromatography-mass spectrometry. American Journal of Applied Chemistry 3: 194-200.
Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Isotopic abundance analysis of biofield treated benzene, toluene and p-xylene using gas chromatography-mass spectrometry (GC-MS). Mass Spectrom Open Access 1: 102.
Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Quantitative determination of isotopic abundance ratio of 13C, 2H, and 18O in biofield energy treated ortho and meta toluic acid isomers. American Journal of Applied Chemistry 3: 217-223.
Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Determination of isotopic abundance of 2H, 13C, 18O, and 37Cl in biofield energy treated dichlorophenol isomers. Science Journal of Analytical Chemistry 4: 1-6.
Hammerschlag R, Jain S, Baldwin AL, Gronowicz G, Lutgendor SK, Oschman JL, Yount GL (2012) Biofield research: A roundtable discussion of scientific and methodological issues. J Altern Complement Med 18: 1081-1086.
Warber SL, Cornelio D, Straughn J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
Rubik B (2002) The biofield hypothesis: Its biophysical basis and role in medicine. J Altern Complement Med 8: 703-717.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield Treatment: A potential strategy for modification of physical and thermal properties of indole. J Environ Anal Chem 2: 152.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of atomic, physical, and thermal properties of bismuth oxide powder: An impact of biofield energy treatment. American Journal of Nano Research and Applications 3: 94-98.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of physical and structural properties of biofield energy treated barium calcium tungsten oxide. Advances in Materials 4: 95-100.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of plant growth, yield and yield attributes of biofield energy treated mustard (Brassica juncea) and chick pea (Cicer arietinum) seeds. Agriculture, Forestry and Fisheries 4: 291-295.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Morphological characterization, quality, yield and DNA fingerprinting of biofield energy treated Alphonso mango (Mangifera indica L.). Journal of Food and Nutrition Sciences 3: 245-250.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Agronomic characteristics, growth analysis, and yield response of biofield treated mustard, cowpea, horse gram, and groundnuts. International Journal of Genetics and Genomics 3: 74-80.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Characterization of phenotype and genotype of biofield treated Enterobacter aerogenes. Transl Med 5: 155.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Phenotyping and 16S rDNA analysis after biofield treatment on Citrobacter braakii: A urinary pathogen. J Clin Med Genom 3: 129.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Evaluation of phenotyping and genotyping characterization of Serratia marcescens after biofield treatment. J Mol Genet Med 9: 179.
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Bacterial identification Using 16S rDNA gene sequencing and antibiogram analysis on biofield treated Pseudomonas fluorescens. Clin Med Biochemistry: Open Access 1: 101.
Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and physical properties of biofield treated bile salt and proteose peptone. J Anal Bioanal Tech 6: 256.
Trivedi MK, Rama MT, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5: 414.
Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Characterisation of physical, spectral and thermal properties of biofield treated resorcinol. Organic Chem Curr Res 4: 146.
Trivedi MK, Branton A, Trivedi D, Nayak G, Singh R, Jana S (2015) Physicochemical and spectroscopic characterization of biofield treated butylated hydroxytoluene. J Food Ind Microbiol 1: 101.
Trivedi MK, Branton A, Trivedi D, Nayak G, Saikia G, Jana S (2015) Physical and structural characterization of biofield treated imidazole derivatives. Nat Prod Chem Res 3: 187.
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) The potential impact of biofield treatment on human brain tumor cells: A time-lapse video microscopy. J Integr Oncol 4: 141.
Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) In vitro evaluation of biofield treatment on cancer biomarkers involved in endometrial and prostate cancer cell lines. J Cancer Sci Ther 7: 253-257.
Rosman KJR, Taylor PDP (1998) Isotopic compositions of the elements 1997 (Technical Report). Pure Appl Chem 70: 217-235.
Smith RM (2004) Understanding Mass Spectra: A Basic Approach, Second Edition, John Wiley & Sons, Inc, ISBN 0-471-42949-X.
Weisel CP, Park S, Pyo H, Mohan K, Witz G (2003) Use of stable isotopically labeled benzene to evaluate environmental exposures. J Expo Anal Environ Epidemiol 13: 393-402.
Jürgen H (2004) Gross Mass Spectrometry: A Textbook (2nd Edn) Springer: Berlin.
Powers JC (1968) Mass spectrometry of simple indoles. J Org Chem 33: 2044-2050.
Gordon J (1998) Inside informatics, cambridgesoft.com Article ID: Isotopic Abundance.
Johnstone RAW, Rose ME (1996) Mass Spectrometry for Chemists and Biochemists (2nd Edn) Cambridge university press.
Wieser ME (2006) Atomic weights of the elements 2005. Pure Appl Chem 78: 2051-2066.
Vanhaecke F, Kyser K (2012) Isotopic composition of the elements In Isotopic Analysis: Fundamentals and applications using ICP-MS (1stedn), Edited by Vanhaecke F, Degryse P. Wiley-VCH GmbH & Co. KGaA, Weinheim.
Asperger S (2003) Chemical Kinetics and Inorganic Reaction Mechanisms Springer science + Business media, New York.
Lomas JS, Thorne MP (1982) Structure and isotope effects upon the thermal decomposition of carbamates of highly congested tertiary alcohols. J Chem Soc, Perkin Trans 2 221-226.
Balantekin AB (2013) Neutrinos and rare isotopes Journal of Physics: Conference Series 445 012022.