Evaluation of Oxidative DNA Damage and Its Relation with Anthropometric Indices in Healthy Adolescents
International Journal of Nutrition and Food Sciences
Volume 4, Issue 2-1, March 2015, Pages: 21-25
Received: Dec. 20, 2014; Accepted: Jan. 25, 2015; Published: Mar. 2, 2015
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Farzaneh Bagherzadeh, Department of Nutrition in Society, Nutrition Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
Mehrangiz Ebrahimi-Mameghani, Department of Nutrition in Society, Nutrition Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
Mohammad Asghari Jafarabadi, Medical Education Research Center, Department of Statistics and Epidemiology, Faculty of Health, Tabriz University of Medical Sciences, Tabriz, Iran
Zahra Aslani, Nutrition and Endocrine Research Center, and Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Sare Edalati, Department of Nutrition in Society, Nutrition Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
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Reactive oxygen species (ROS) and oxidative stress (OS) have important role in prevention, initiation and progression of chronic diseases from early childhood, but OS status in healthy young subjects and appropriate methods for its measurement have been remained unclear. Evaluation of urinary biomarker set for OS has not been well documented in Iran. In this study, evaluation of OS from urinary biomarker in healthy adolescents and its relevance to anthropometric index were investigated. Forty- hundred students aged 13 and 19 years old participated in this study. 8-hydroxy-2ʹ-deoxyguanosine (8-OHdG) as marker of oxidative DNA damage was measured in adolescents and also its relation with age, sex and body mass index was studied. Subjects were sampled by multistage cluster sampling. The average ratio of 8-OHdG to creatinine was 4.7±4.1.No significant difference of 8-OHdG was observed between boys and girls in this study.
Oxidative Stress, Oxidative DNA Damage, Adolescents, Body Mass Index, Age, Sex
To cite this article
Farzaneh Bagherzadeh, Mehrangiz Ebrahimi-Mameghani, Mohammad Asghari Jafarabadi, Zahra Aslani, Sare Edalati, Evaluation of Oxidative DNA Damage and Its Relation with Anthropometric Indices in Healthy Adolescents, International Journal of Nutrition and Food Sciences. Special Issue: Functional Foods and Nutraceuticals for Management of Type 2 Diabetes. Vol. 4, No. 2-1, 2015, pp. 21-25. doi: 10.11648/j.ajbio.s.2015030202.11
Rahman I, Kelly F. Review Biomarkers in Breath Condensate: A promising New Non-invasive Technique in Free Radical Research. FRA. 2003;37(12):1253-66.
Offord E, Van Poppel G, Tyrrell R. Markers of oxidative damage and antioxidant protection: current status and relevance to disease. FRA. 2000;33:S5.
Nemeth I, Boda D. Xanthine oxidase activity and blood glutathione redox ratio in infants and children with septic shock syndrome. JIC. 2001;27(1):21-26.
Tsukahara H, Sekine K, Uchiyama M, Kawakami H, Hata I, Todoroki Y, et al. Formation of advanced glycosylation end products and oxidative stress in young patients with type 1 diabetes. Pediatr. Res.. 2003;54(3):419-24.
Kaneko K, Taniguchi N, Tanabe Y, Nakano T, Hasui M, Nozu K. Oxidative imbalance in idiopathic renal hypouricemia. Pediatr Neph. 2009;24(4):869-71.
Tung EW, Winn LM. Valproic acid increases formation of reactive oxygen species and induces apoptosis in postimplantation embryos: a role for oxidative stress in valproic acid-induced neural tube defects. Mol. Pharmacol.. 2011;80(6):979-87.
Granot E, Kohen R. Oxidative stress in childhood-in health and disease states. Clin Nutr. 2004;23:3-11.
Toyokuni S, Tanaka T, Hattori Y, Nishiyama Y, Yoshida A, Uchida K, et al. Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45. 1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model. Laboratory investigation; Lab Invest. 1997;76(3):365-74.
Jang-Young K, Jun-Won L, YounMin-Soo A, Sung-Gyun A. Urinary Levels of 8-Iso-Prostaglandin F2α and 8-Hydroxydeoxyguanine as Markers of Oxidative Stress in PatientsWith Coronary Artery Disease. Korean Circ J. 2012;42:614-7.
Valavanidis A, Vlachogianni T, Fiotakis C, C JESH. 8-Hydroxy-2′-deoxyguanosine (8-OHdG): acritical biomarker of oxidative stress and carcinogenesis. 2009;27:120-39.
Wu LL, Chiou C-C, Chang P-Y, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clinica Chimica Acta. 2004;339(1):1-9.
Subash P, Gurumurthy P, Sarasabharathi A, Cherian K. Urinary 8-OHdG: a marker of oxidative stress to dna and total antioxidant status in essential hypertension with South Indian population. IJCB. 2010;25(2):127-32.
Hwang E-S, Kim G-H. RETRACTED: Biomarkers for oxidative stress status of DNA, lipids, and proteins in vitro and in vivo cancer research. Toxicology. 2007;229(1):1-10.
Sakano N, Wang D-H, Takahashi N, Wang B, Sauriasari R, Kanbara S, et al. Oxidative stress biomarkers and lifestyles in Japanese healthy people. JCBN2009;44(2):185.
Halliwell B. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am Clin Nutr. 2000;72(5):1082-7.
Drury JA, Jeffers G, Cooke RW. Urinary 8-hydroxydeoxyguanosine in infants and children. FRA. 1998; 28(4): 423-8.
Kaneko K, Kimata T, Tsuji S, Ohashi A, Imai Y, Sudo H, et al. Measurement of urinary 8-oxo-7,8-dihydro-2-deoxyguanosine in a novel point-of-care testing device to assess oxidative stress in children. Clinica Chimica Acta. 2012;413:1822-6.
Tamura S, Tsukahara H, Ueno M. Evaluation of a urinary multi-parameter biomarker set for oxidative stress in children, adolescents and young adults. FreeRadic Res. 2006;40:1198-205.
Tsukahara H. Biomarkers for oxidative stress: clinical application in pediatric medicine. Curr Med Chem. 2007; 14:339-51.
Piconi L, Quagliaro L, Ceriello A. Oxidative stress in diabetes. Clinical chemistry and laborator Med. 2003;41(9):1144-9.
Kasai H, Kawai K, Li Y-s. Analysis of 8-OH-dG and 8-OH-Gua as biomarkers of oxidative stress. Genes and Environment. 2008;30(2):33-40.
Tamura S, Tsukahara H, Ueno M, Maeda M, Kawakami H, Sekine K, et al. Evaluation of a urinary multi-parameter biomarker set for oxidative stress in children, adolescents and young adults. FRA. 2006;40(11):1198-205.
Ainsworth BE, Haskell WL, Whitt MC, Irwin ML, Swartz AM, Strath SJ, et al. Compendium of physical activities: an update of activity codes and MET intensities. MSSE. 2000;32(9; SUPP/1):S498-S504.
Poulsen HE, Loft S, Prieme H, Vistisen K, Lykkesfeldt J, Nyyssonen K, et al. Oxidative DNA damage in vivo: relationship to age, plasma antioxidants, drug metabolism, glutathione-S-transferase activity and urinary creatinine excretion. FRA. 1998;29(6):565-71.
Chiavaroli V, Giannini C, De Marco S, Chiarelli F, Mohn A. Unbalanced oxidant–antioxidant status and its effects in pediatric diseases. Redox Report. 2011;16(3):101-7.
Kauffman LD, Sokol RJ, Jones RH, Awad JA, Rewers MJ, Norris JM. Urinary F 2-isoprostanes in young healthy children at risk for type 1 diabetes mellitus. FRB M. 2003;35(6):551-7.
Andreazza AC, Bordin DL, Salvador M. Thiobarbituric acid reactive substances, seric superoxide dismutase and catalase activities in healthy subjects. Clinica Chimica Acta. 2005; 362(1):192-4.
Miles MV, Horn PS, Tang PH, Morrison JA, Miles L, DeGrauw T, et al. Age-related changes in plasma coenzyme Q 10 concentrations and redox state in apparently healthy children and adults. Clinica Chimica Acta. 2004; 347(1):139-44.
Atabek ME, Vatansev H, Erkul I. Oxidative stress in childhood obesity. J PEM. 2004; 17(8): 1063-8.
Evans MD, Dizdaroglu M, Cooke MS. Oxidative DNA damage and disease: induction, repair and significance. Mutation Research/Reviews in Mutation Research. 2004; 567(1): 1-61.
Chiou C-C, Chang P-Y, Chan E-C, Wu T-L, Tsao K-C, Wu JT. Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clinica Chimica Acta. 2003; 334(1):87-94.
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