Bioactive Properties of Japanese Fermented Fish Paste, Fish Miso, Using Koji Inoculated With Aspergillusoryzae
This research was evaluated the antioxidant activity of fish miso, a newly developed fermented fish paste prepared from horse mackerel meat and the fermentation of traditional Japanese koji inoculated with Aspergillusoryzae. The antioxidant activities in different in vitro models, including DPPH, hydroxyl, nitric oxide and carbon-centered radical-scavenging activity (RSA), and reducing power ability (RPA), were investigated during the fermentation period along with 2 different storage conditions. The antioxidant activity of matured fish miso was also evaluated using a linoleic acid oxidation model system by monitoring hydrogen peroxide formation and oxygen absorption. The RSA against all the types of radicals measured by electron spin resonance showed an increase over prolonged fermentation periods and during storage at high temperatures. However, the RPA showed a rapid increase during the early stages of fermentation. The SDS-PAGE profile of fish miso peptides during the early stages of fermentation indicated the occurrence of hydrolysis, suggesting the involvement of low-molecular-weight peptides in the RSA and reducing power of fish miso. Partial purification of these peptides by using an online-HPLC-DPPH flow injection analysis system and further characterization by thin layer chromatography and molecular weight distribution clearly indicated that low-molecular-weight peptides (<500 Da) were potent antioxidants. These data suggest that the antioxidant activity of fish miso could be substantially improved by fermenting Aspergillus (koji) mold. This approach provides a novel strategy to enhance the value of trash fish, such as horse mackerel.
Bioactive Properties of Japanese Fermented Fish Paste, Fish Miso, Using Koji Inoculated With Aspergillusoryzae, International Journal of Nutrition and Food Sciences.
Vol. 1, No. 1,
2012, pp. 13-22.
J. P. Kehrer, "Free radicals as mediators of tissue injury and disease," Crit. Rev. Toxicol., vol. 23, 1993, pp. 21–48.
N. Rajapakse, E. Mendis, W. K. Jung, J. Y. Je, and S. K. Kim, "Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties," Food Res. Int., vol. 38, 2005, pp. 175–182.
N. GokturkBaydar, G. Ozkan, and S. Yasar, "Evaluation of the antiradical and antioxidant potential of grape extracts," Food Control, vol. 18, 2007, pp. 1131–1136.
E. Berghofer, B. Grzeskowiad, N. Mundigler, W. B. Sentall, and J. Walcak, "Antioxidant properties of faba bean-, soybean-, soybean and oat tempeh," Int. J. Food Sci. Nutri., vol. 49, 1998, pp. 45–54.
B. F. Gibbs, A. Zougman, R. Masse, and C. Mulligan, "Production and characterization of bioactive peptides from soy hydrolysate and soy-fermented food," Food Res. Int., vol. 37, 2004, pp. 123–131.
L. A. Santiago, M. Hiramatsu, and A. Mori, "Japanese soybean paste miso scavenges free radicals and inhibits lipid peroxidation," J. Nutri. Sci. Vitaminol., vol. 38, 1992, pp. 297–304.
A. Kanda, Y. Hoshiyama, and T. Kawaguchi, "Association of lifestyle parameters with the prevention of hypertension in elderly Japanese men and women: a four-year follow-ip of normotensive subjects," Asia Pacific J. Public Health, vol. 11, 1999, pp. 77-81.
M. Ohara, H. Lu, K. Shiraki, Y. Ishimura, T. Uesaka, O. Katoh, andH. Watanabe, "Prevention by long-term fermented miso of induction of colonic aberrant crypt foci by azoxymethane in F344 rats," Oncol. Rep., vol. 9, 2002, pp. 69-73.
T. Kanazawa, T. Osanai, X.S. Zhang, T. Uemura, X.Z. Yin, K. Onoder, Y. Oike, and K. Ohkubo, "Protective effects of soy protein on the peroxidizability of lipoproteins in cerebrovascular disease," J. Nutri., vol. 125, 1995, pp. 639S-646S.
M. Horii, T. Ide, K. Kawashima, and T. Yamamoto, "Hypo-cholesterolemic activity of desalted miso in rats fed an atherogenic diet," Nippon ShokuhinKogakuKaishi, vol. 37, 1990, pp. 148-153.
D. D. Sastraatmadja, F. Tomita, and T. Kasai, "Production of high-quality oncom, a traditional Indonesian fermented food, by the inoculation with selected mold strains in the form of pure culture and solid inoculum," J. Graduate School of Agriculture, Hokkaido University, vol. 70, 2002, pp. 111-127.
M. Matsuo, "Preparation and components of okara-ontjom, a traditional Indonesian fermented food," Nippon Shokuhin Kogyo Gakkaishi, vol. 44, 1997, pp. 632-639 (in Japanese).
M. Matsuo and Y. Yumoto, "Preparation of tasty improved defatted soybean Ontjoms (fermented products with Neurosporaintermedia)," Food Sci. Technol. Res., vol. 5, 1999, pp. 168-170.
M. Matsuo, "Antioxidant activity of fermented defatted soybean with Neurosporaintermedia, D-Ontjom," J. Jap. Soc. Nutri. Food Sci., vol. 54, 2001, pp. 305-309 (in Japanese).
M. Matsuo, "Antioxidant activity of hydrophilic compounds of defatted soybean fermented with Neurosporaintermedia (D-Ontjom)," Food Sci. Technol. Res., vol. 8, 2002, pp. 253-238.
M. Matsuo, "Plasma cholesterol reduction by defatted soy Ontjom (fermented with Neurosporaintermedia) in rats fed a cholesterol-free diet," J. Nutri. Sci. Vitaminol., vol. 46, 2000, pp. 30-33.
M. Matsuo, "Plasma cholesterol reducing effect by okaraoncom (fermented with Neurosporaintermedia) in rats fed a cholesterol-free diet," ITE Lett. Batt., New Technol. Medicine, vol. 3, 2002, pp. 732-735.
J. W. Bennett, "Taxonomy of fungi and biology of the Aspergillus," in Biology of Industrial Microorganisms, A. L. Demain and N. A. Solomon, Eds. London: The Benjamin/ Cummings Publishing Company, Inc., 1985, pp. 359-406.
J. W. Bennett and M. A. Klich, "Aspergillus: Biology and industrial applications," in J. W. Bennett and M. A. Klich, Eds. 1992, pp. 134-137.
K. B. Raper and D. I. Fennell, "The Genus Aspergillus," Baltimore: Williams and Wilkins, 1965, p. 686.
Z. Kozakiewicz, "Aspergillus Species on Stored Products," Mycol. Pap., vol. 161, 1989, pp. 1-187.
C. W. Hesseltine, "Future of fermented foods," Process Biochem., vol. 16, 1981, pp. 2-16.
M. Raimbault and D. Alazard, "Culture method to study fungal growth in solid fermentation," Eur. J. Appl. Microbiol. Biotechnol., vol. 9, 1980, pp. 199-209.
L. J. Yin, Y. L. Tong, and S. T. Jiang, "Improvement of the functionality of minced mackerel by hydrolysis and subsequent lactic acid bacterial fermentation," J. Food Sci., vol. 70, 2005, pp. 172-178.
L. J. Yin, Y. L. Tong, and S. T. Jiang, "Effect of combining proteolysis and lactic acid bacterial fermentation on the characteristics of minced mackerel," J. Food Sci., vol. 70, 2005, pp. 186-192.
F. Nanjo, K. Goto, R. Seto, M. Susuki, M. Sakai and Y. Hara, "Scavenging effects of Tea catechins and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical," Free Radic. Biol. Med., vol. 21, 1996, pp. 895–902.
G. M. Rosen and E. J. Rauckman, "Spin trapping of superoxide and hydroxyl radicals," in Methods in enzymology, vol. 105, L. Packer, Ed. Orlando: Academic Press, 1984, pp. 189–209.
K. Hiramoto, H. Johkoh, K. I. Sako and K. Kikugawa, "DNA breaking activity of the carbon-centered radical generated from 2,2-azobis(2 amidinopropane) hydrochloride (AAPH)," Free Radic. Res. Commun., vol. 19, 1993, pp. 323–332.
M. Oyaizu, "Antioxidant activities of browning reaction prepared from glucosamine," Jap. J. Nutri., vol. 44, 1986, pp. 307–315.
T. Osawa and M. Namiki, "Natural antioxidnts isolated from eucalyptus leaf waxes," J. Agric. Food Chem., vol. 33, 1985, pp. 777–780.
J. H. Sohn, Y. Taki, H. Ushio, and T. Ohshima, "Quantitative determination of total lipid hydroperoxides by a flow injection analysis system" Lipids, vol. 40, 2005, pp. 203–209.
T. Ohshima, N. Li, and C. Koizumi, "Oxidative decomposition of cholesterol in fish products," J. Am. Oil Chem. Soc., vol. 70, 1993, pp. 595-600.
C. L. Jao and W. C. Ko, "1,1-dipheny1-2-picrylhydrazyl (DPPH) radical scavenging by protein hydrolyzates from Tuna cooking juice," Fish. Sci., vol. 68, 2002, pp. 430–435.
L. Yu, S. Haley, J. Perret, M. Harris, J. Wilson, and M. Qian, "Free radical scavenging properties of wheat extracts," J. Agric. Food Chem., vol. 50, 2002, pp. 1619–1624.
E. A. Hagerman, K. M. Riedl, G. A. Jones, K. N. Sovik, N. T. Ritchard, P. W. Hartzfeld, and T. L. Riechel, "High molecular weight plant polyphenolics (tannins) as biological antioxidants," J. Agric.Food Chem., vol. 46, 1998, pp. 1887–1892.
B. Halliwell and J.M. Gutteridge, "Role of free radicals and catalytic metal ions in human disease: an overview," Methods Enzymol., vol. 186, 1990, pp. 1–85.
P. D. Duh, "Antioxidant activity of burdock (ArctiumlappaLinne):Its scavenging effect on free radical and active oxygen," J. Am. Oil Chem. Soc., vol. 75, 1998, pp. 455–465.
P. D. Duh, Y. Y. Tu, and G. C. Yen, "Antioxidant activity of waterextract of HarngJyur (Chrysanthemum morifolium Ramat)," LWT Food Sci. Tech., vol. 32, 1999, pp. 269–277.
S. Ohtaki, H. Maeda, T. Takahashi, Y. Yamagata, F. Hasegawa, K. Gomi, T. Nakajima, and K. Abe, "Novel hydrophobic surface binding protein, HsbA, produced by Aspergillusoryzae," Appl. Environ. Microbiol., Apr. 2006, pp. 2407–2413.
M. R. Rashid, M. R. Javed, T. Kawaguchi, J. Sumitani, and M. Arai, "Improvement of Aspergillusoryzae for hyperproduction of endoglucanase: expression cloning of cmc-1 gene of Aspergillusaculeatus," Biotechnol. Lett., vol. 30, 2008, pp. 2165–2172.
N. Rajapakse, E. Mendis, H. G. Byun, and S. K. Kim, "Purification and in vitro antioxidative effects of giant squid musclepeptides on free radical-mediated oxidative systems," J. Nutri. Biochem., vol. 16, 2005, pp. 562–569.
Y. C. Chung, C. T. Chang, W. W. Chao, C. F. Lin, and S. T. Chou, "Antioxidant activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1," J. Agric. Food Chem., vol. 50, 2002, pp. 2454–2458.
R. Marcuse, "The effect of some amino acids on oxidation of linoleic acid and its methyl esters," J. Am. Oil Chem. Soc., vol. 39, 1962, pp. 97–103.
M. Karel, S. R. Tannenbuam, D. H. Wallace, and H. Maloney, "Autoxidation of methyllinoleate in freeze-dried model systems. III.Effects of added amino acids," J. Food Sci., vol. 31, 1966, pp. 892-896.
N. Yamaguchi, Y. Yokoo, and M. Fujimaki, "Antioxidative activities of protein hydrolyzates," Nippon Shokuhin Kogyo Gakkaishi, vol. 26, 1979, pp. 65-70.
A. M. Wade and H. N. Tucker, "Antioxidant characteristics of L-histidine," J. Nutri. Biochem., vol. 9, 1998, pp. 308–315.
H. M. Chen, K. Muramoto, and F. Yamauchi, "Structural analysis of antioxidative peptides from soybean b-conglycinin," J. Agric. Food Chem., vol. 43, 1995, pp. 574–578.
H. M. Chen, K. Muramoto, F. Yamauchi, and K. Nokihara, "Antioxidant activity of design peptides based on the antioxidative peptide isolated from digests of a soybean protein," J. Agric. Food Chem., vol. 44, 1996, pp. 2619–2623.
K. Suetsuna, H. Ukeda, and H. Ochi, "Isolation and characterization of free radical scavenging activities peptides derived from casein," J. Nutri. Biochem., vol. 11, 2000, pp. 128–131.
J. F. Fan, M. Saito, Y. Y. Zhang, S. S. Tan, L. J. Wang, and E. Tatsumi, "Gel-forming ability and radical-scavenging activity of soy protein hydrolysate treated with transglutaminase," J. Food Sci., vol. 70, 2005, pp. 87–92.
Y. C. Wang, R. C. Yu, and C. C. Chou, "Antioxidant activities of soymilk fermented with lactic acid bacteria and bifidobacteria," Food Microbiol., vol. 23, 2006, pp. 128–135.
S. Y. Kim, J. Y. Je, and S. K. Kim, "Purification and characterization of antioxidant peptide from hoki (Johniusbelengerii) frame protein by gastrointestinal digestion," J. Nutri. Biochem., vol. 18, 2007, pp. 31–38.