Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats
International Journal of Nutrition and Food Sciences
Volume 2, Issue 2, March 2013, Pages: 24-30
Received: Feb. 1, 2013; Published: Mar. 10, 2013
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Mostafa M. Osfor, Clinical Nutrition Department, Applied Medical Sciences College, Umm Al-Qura University, Makkah, Saudi Arabia
Osama Kensarah, Clinical Nutrition Department, Applied Medical Sciences College, Umm Al-Qura University, Makkah, Saudi Arabia
Firas Azzeh, Clinical Nutrition Department, Applied Medical Sciences College, Umm Al-Qura University, Makkah, Saudi Arabia
Mohammad A. Elmadbouly, Clinical Nutrition Department, Applied Medical Sciences College, Umm Al-Qura University, Makkah, Saudi Arabia
Mona El-Qutry, Nutrition and Food science, Home Economic Collage, Ain Shame University, Cairo, Egypt
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The effect of high fat diet on lipid and carbohydrate metabolic changes was studied in mature male albino rats. The animals were rendered obese by feeding a high fat diet for 60 days. Half of obese rats were become diabetic by injecting with a reduced dose of alloxan; to produce a non insulin dependent diabetes mellitus state. These obese models of non diabetic and diabetic rats were compared with normal male rats kept on a basal diet for 60 days. The fat fed rats beside gaining weight showed a significant rise in their hepatic lipid contents and observed an insulin resistant state. Lipid profile of the omental adipose tissue exhibited a significantly higher total lipid content, but lower total cholesterol, triglycerides and phospholipids contents in both obese (non diabetic and diabetic) as compared with the normal rats. On the other hand, the brown adipose tissue showed a reduced fat catabolism with a significant rise in its phospholipid contents. Correlation between plasma atherogenic index and plasma fatty acids pattern showed that in the obese diabetic rats atherogenic index was positively correlated with total polyunsaturated fatty acids, while significantly negative correlation with total saturated fatty acids. This work showed that high fat substrates have been got remarkable impacts on rats body weight and metabolism.
Obesity, Diabetes, Male Albino Rats
To cite this article
Mostafa M. Osfor, Osama Kensarah, Firas Azzeh, Mohammad A. Elmadbouly, Mona El-Qutry, Biochemical Changes in LIPID and Car-bohydrate Metabolism in Obese Diabetic Male Albino Rats, International Journal of Nutrition and Food Sciences. Vol. 2, No. 2, 2013, pp. 24-30. doi: 10.11648/j.ijnfs.20130202.11
M. Croze, R. Vella, N. Pillon, H. Soula, L. Hadji, M. Gui-chardant, and C. Soulage, "Chronic treatment with myo-inositol reduces white adipose tissue accretion and im-proves insulin sensitivity in female mice", The Journal of Nutritional Biochemistry, 2013, 24(2): 457–466.
T. Pischon, H. Boeing, and K. Hffmann, "General and ab-dominal adiposity and risk of death in Europe", N. Engl. J. Med. 2008, 359(20): 2105-2120.
L. Storlien, A. Jenkkins, D. Chisholm, W. Pascoe, S. Khouri, and E. Kraegen, (1991): "Influence of dietary fat composition on development of insulin resistance in rats relationship to muscle triglycerides and n-3 fatty acids in muscles phospho-lipids", Diabetes, 40: 280-289.
T. Pelinkanova, M. Kohout, J. Valek, J. Base, and L. Kazdova, "Insulin secretion and insulin action related to the serum phospholipids fatty acids pattern in healthy men", Metabolism, 1989, 38(2): 188-192.
E. Berry, J. Hirsch, J. Most, D. McNamara, and J. Thornton, "The relationship of dietary fat to plasma lipid levels as studied by factor analysis of adipose tissue fatty acid composition in a free-living population of middle aged American men", Am. J. Clin. Nutr. 1986, 44: 220-231.
J. Triscari, N. Koral, B. Levin, and A. Sullivan, "Changes in lipid metabolism in diet-induced obesity", Metabolism 1985, 34(6): 580-587.
M. Osfor, "Protective role of green Tea and Panax ginseng in cancer-promoting Diets: a sub-chronic study on the male albino rats", Bull. NRC, Egypt, 2003, 28(5): 591 – 621.
J. Folch, and G. Stanley, "A simple method for the isolation and purification of total lipids from animal tissue", J. boil. Chem. 1957, 226: 497.
G. Siest, J. Henny, and F. Schiele, (1981): Interpretation des examens de Laboratorire. Karger ed. pp: 206-223.
R. Yalow, and W. Bouman, Plasma Insulin in Health and Disease. In: Diabetes mellitus: Theory and Practice, M. Edinberg and H. Rifkin (Eds.), Excerpta Medica, New York, pp: 119-150, 1983.
J. Schmit, and B. Drevon, "Sulfo-phospho-Vanillic reaction in lipid and lipoprotein evaluation in baboon serum comparison with human serum", Compt. Rend. Soc. Boil. 1964, 158(4), 778-780.
V. Dole, "Fractionation of plasma non- esterifies fatty acids Proc", Soc. Exptl. Biol. Med. 1956, 93: 532-533.
P. Fossati, and L. Prencipe, "Serum triglycerides determined calorimetrically with an enzyme that produces hydrogen peroxide", Clin. Chem. 1982, 28: 2077.
E. King, and I. Woolton, Microanalysis in Medical Bioche-mistry, 3rd ed. Churchill LTD: London, 1956.
C. Allain, L. Poon, C. Chan, W. Richmond, and P. Fu, "En-zymatic determination of total cholesterol", J. Clin. Chem. 1974, 20:470-475.
C. Ratliff, and F. Hall, Laboratory Manual of Clinical Bio-chemistry. USA, Scott and White Memorial Hospital Publi-cations Office Temple, IX, 1977.
M. Lopez-Virella, P. Stoine, S. Ellis, and J. Acollwall, "Cholesterol determination in high density lipoprotein by three different methods", Clin. Chem. 1977, 53(5): 882-884.
T. Glatter, "Hyperlipidemia, What is normal, who should be treated and how?", Post-Grad Med. 1984, 76(6): 49-59.
P. Armitage, "Statistical Methods in Medical Research", 3rd ed. Blackwell scientific Publications, Oxford and Einburgh, England, 1974.
C. Lavie, R. Milani, and H. Ventura, "Obesity and cardi-ovascular disease; risk factor, Paradox, and impact of weight loss", J. Am. Coll. Cardiol. 2009, 53: 1925-1932.
S. Grundy, J. Cleeman, and R. Daniels, "Diagnosis and management of the metabolic syndrome, Circulation, 2005, 112: 2735-3752.
S. Grundy, B. Hansen, and Jr S. Smith, "American heart association; national heart, Lung and blood Institute; American diabetes association 2004 Clinical management of the metabolic syndrome: report of the American Heart association/ National heart, lund, and Blood institute/ American Diabetes association Conference on scientific issues related to management", Circulation, 2004, 109: 551-556.
J. Baker, L. Olsen, and T. Soprensen, "Childhood body-mass index and the risk of coronary heart disease in adulthood", N. Engl. J. Med. 2007, 357: 2329-2337.
A. Oreopoulos, R. Paswal, and K. Kalantar-zadeh, "Body mass index and mortality in heart failure: a meta analysis", Am. Heart J. 2008, 156: 13-22.
L. Barness, J. Opitz, and E. Gilbert-Barness, "Obesity: genetic, molecular, and environmental aspects", Am. J. Med. Genet. 2007, 143A (24): 3016-34.
T. Constantine, H. Vojtech, B. Arnaud, F. Nick, F. Martin, M. Elisabeth, M. Dragan, and M. Mximo, "Management of ob-esity in adults: European Clinical Practice Guidelines", The European Journal of Obesity, 2008, 1(2): 106-116.
R. Ness-Abramof, and C. Apovian, "Diet modification for treatment and prevention of obesity", Endocrine, 2006, 29 (1): 5-9.
P. De Varies, H. Folkers, and C. Defijter, "Adipose tissue fatty acid composition and its relation to diet and plasma lipid concentrations in haemodialysis patients", Am. Clin. Nutr. 1991, 53:468-473.
G. Whitlock, S. Lewington, and P. Sherliker, "Body mass index and cause specific mortality in 900 000 adults: Colla-borative analysis of 57 prospective studies", Lancet, 2009, 373: 1083-1096.
R. Brownson, T. Boehmer, and D. Luke, "Declining rates of physical activity in the united states: what are the contributors?", Annu Rev. public Health, 2005, 26: 421-443.
K. Borodulin, T. Laatikainen, A. Juolevi, and P. Jousilahti, "Thirsty year trends of physical activity in relation to age, calendar time and birth cohort in finnish adults", Eur. J. Public Health, 2008, 18 (3): 339-344.
M. Fried, V. Hainer, and A. Basdevnt, "Inter-disciplinary European guidelines on surgery of severs obesity", Int. J. Obes. (Lond), 2007, 31(4): 569-577.
I. Cusin, F. Rohner-Jeanreenaud, J. Jenettaz, and B. Jeanrenaud, "Hyperinsulinemia and its impact on obesity and insulin resistance", Int. JK, 1992, Obesity 16 (4), SI-SII.
W. Mckane, A. Stevers, R. Woods, W. Andrews, R. Henry, and P. Bell, "The assessment of hepatic and peripheral insulin sensitivity in htpertriglyceridemia", Metabolism, 1990, 39 (12), 1240-1245.
R. Harris, and H. Kor, "Insulin insensitivity is rapidly reversed in rats by reucing dietary fat from 40 to 30 % of energy", J Nutr. 1992, 122: 1811-1822.
R. Krauss, M. Winston, B. Fletcher, and S. Graundy, "Obesity is not an independent risk factor for adverse peri-operative and long-term Clinical outcomes following Open AAA Repair or Evar VASC", Endovascular Surg. 2011, 45: 607-613.
E. Schaefer, R. Levy, N. Renst, F. Van sant, and H. Brewer, "The effect of low cholesterol, high polyunsaturated fat, and low fat diet on plasma lipids and cholesterol levels in normal and hypercholesterolemic subjects", Am. J. Clin. Nutr. 1981, 34, 1758-1763.
M. Osfor, W. Nicola, E. Badawy, and M. Emam, "Composition of plasma lipoproteins and their impact on metabolic complication in fatness rats treated with metformin and ni-cotinic acid", Med. J. Cairo Univ., 1999, 67(1) (suppl.) 59 – 73.
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