Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells
International Journal of Nutrition and Food Sciences
Volume 2, Issue 4, July 2013, Pages: 167-173
Published: Jun. 20, 2013
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Rungtip Soi-ampornkul, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Chatchawan Srisawat, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Wipawan Thangnipon, Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand
Surin Kanyok, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Sarawut Junnu, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Wanphen Katanyoo, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Sompong Liammongkolkul, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Pairat Soi-ampornkul, Surasak montree school, The Ministry of Education, Wipawadee road, Huaikhwang, Bangkok, Thailand
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Amyloid β-peptide (Aβ1-42) is the major component of amyloid plaques and is believed to induce oxidative stress and inflammation in brain, which are postulated to play important roles in the pathogenesis of Alzheimer’s disease (AD). The present study investigated the effect of pre-germinated brown rice extract (Oryza sativa L., PGBR ex) which contains a much higher concentration of essential amino acids, such as lysine, isoleucine, methionine and several kinds of antioxidants than conventional brown rice, and over 8-13 times the amount of γ-aminobutyric acid (GABA) shown to protect against oxidative stress and cell death. Pre-treatment of SK-N-SH cells with 2000 μg/ml PGBR ex significantly attenuated 10 μM Aβ1-42-induced neuronal cells deaths. Apoptotic cells death was demonstrated morphologically as well as by detection of the presence of activated caspase-3, DNA fragmentation and the production of reactive oxygen species (ROS) which could be reduced by pre-treatment of PGBR ex and brown rice extract (BR ex). These results suggest that the protective effect of PGBR ex and BR ex against Aβ1-42-induced neuronal cell death might be due to its anti-oxidative and anti-apoptotic properties and could be useful as a value added functional food to prevent neurodegenerative diseases.
Brown Rice Extract, SK-N-SH Cells, Antioxidant, GABA, ROS, Aβ1-42, Apoptosis, DNA Fragmentation
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Rungtip Soi-ampornkul, Chatchawan Srisawat, Wipawan Thangnipon, Surin Kanyok, Sarawut Junnu, Wanphen Katanyoo, Sompong Liammongkolkul, Pairat Soi-ampornkul, Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells, International Journal of Nutrition and Food Sciences. Vol. 2, No. 4, 2013, pp. 167-173. doi: 10.11648/j.ijnfs.20130204.12
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