Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells
International Journal of Nutrition and Food Sciences
Volume 2, Issue 4, July 2013, Pages: 167-173
Published: Jun. 20, 2013
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Rungtip Soi-ampornkul, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Chatchawan Srisawat, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Wipawan Thangnipon, Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand
Surin Kanyok, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Sarawut Junnu, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Wanphen Katanyoo, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Sompong Liammongkolkul, Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand
Pairat Soi-ampornkul, Surasak montree school, The Ministry of Education, Wipawadee road, Huaikhwang, Bangkok, Thailand
Amyloid β-peptide (Aβ1-42) is the major component of amyloid plaques and is believed to induce oxidative stress and inflammation in brain, which are postulated to play important roles in the pathogenesis of Alzheimer’s disease (AD). The present study investigated the effect of pre-germinated brown rice extract (Oryza sativa L., PGBR ex) which contains a much higher concentration of essential amino acids, such as lysine, isoleucine, methionine and several kinds of antioxidants than conventional brown rice, and over 8-13 times the amount of γ-aminobutyric acid (GABA) shown to protect against oxidative stress and cell death. Pre-treatment of SK-N-SH cells with 2000 μg/ml PGBR ex significantly attenuated 10 μM Aβ1-42-induced neuronal cells deaths. Apoptotic cells death was demonstrated morphologically as well as by detection of the presence of activated caspase-3, DNA fragmentation and the production of reactive oxygen species (ROS) which could be reduced by pre-treatment of PGBR ex and brown rice extract (BR ex). These results suggest that the protective effect of PGBR ex and BR ex against Aβ1-42-induced neuronal cell death might be due to its anti-oxidative and anti-apoptotic properties and could be useful as a value added functional food to prevent neurodegenerative diseases.
Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells, International Journal of Nutrition and Food Sciences.
Vol. 2, No. 4,
2013, pp. 167-173.
Behl, C., 1999. Alzheimer's disease and oxidative stress: implications for novel therapeutic approaches. Prog.Neurobiol. 57, 301-323.
McGeer, P.L., McGeer E.G., 1999. Inflammation of the brain in Alzheimer's disease: implications for therapy. J. Leukoc Biol. 65, 409-415.
Behl, C., Davis, J.B., Lesley, R., Schubert, D., 1994. Hydrogen peroxide mediates amyloid protein toxicity. Cell. 77, 817-827.
McDonald, D.R., Brunden, K.R., Landreth, G.E., 1997. Amyloid fibrils activate tyrosine kinase-dependent signaling and superoxide production in microglia. J. Neurosci.17, 2284-2294.
Araujo, D.M., Cotman, C.W., 1992.-amyloid stimulates glial cells in vitro to produce growth factors that accumulate in senile plaques in Alzheimer's disease. Brain Res.569:141-145.
Gitter, B.D., Cox, L.M., Rydel, R.E., May, P.C., 1995. Amyloid peptide potentiates cytokine secretion by interleukin-1 beta-activated human astrocytoma cells. Proc. Natl. Acad. Sci. USA. 92,10738-10741.
Behl, C., Davis, J., Cole, G.M., Schubert, D., 1992. Vitamin E protects nerve cells from amyloid beta protein toxicity. BiochemBiophys Res. Commun.186, 944-950.
Yamada. K., Tanaka, T., Han, D., Senzaki, K., Kameyama. T., Nabeshima, T., 1999. Protective effects of idebenone and alpha-tocopherol on -amyloid-(1-42)-induced learning and memory deficits in rats: implication of oxidative stress in -amyloid-induced neurotoxicity in vivo. Eur. J. Neurosci. 11, 83-90.
Sano, M., Ernesto, C., Thomas, R.G., Klauber, M.R., Schafer, K., Grundman, M., et al., 1997. A controlled trial of selegiline, alpha-tocopherol, or both as treatment for Alzheimer's disease. The Alzheimer's Disease Cooperative Study. N. Engl. J. Med.336, 1216-1222.
Katyama, M., Yoshimi, N., Yamada, Y., Sakata, K., Kuno, T., Yoshida, K., Qiao, Z., Vihn, P.Q., Iwasaki, T., Kobayash, H., Mori, H., 2002. Preventive effect of fermented brown rice and rice bran against colon carcinogenesis in male F344 rats. Oncology. Rep.9, 817-822.
Oh, S.H., Kim, S.H., Moon, Y.J., Choi, W.G., 2002. Changes in the levels of γ-aminobutyric acid and some amino acids by application of glutamic acid solution for the germination of brown rices. Korean J. Biotechnol. Bioeng.17, 49-53.
Oh, S.H., 2003. Stimulation of γ-aminobutyric acid synthesis activity in brown rice by a chitosan/glutamic acid germination solution and calcium/calmodulin. J.Biochem. Mol. Biol.36, 319-325.
Latifah, et.Al., 2010. Germinated brown rice (GBR) reduces the incidence of aberrant crypt foci with the involvement of β-catenin and COX-2 in azoxymethane-induced colon cancer in rats. Nutrition Journal. 9, 9-16.
Zhang, R., Lu, H., Tian, S., Yin, J., Chen,O., M, L., Cui, S., Niu, Y., 2010. Protective effects of pre-germinated brown rice diet on low levels of Pb-induced learning and memory deficits in developing rat.Chemico-Biological Interactions.184, 484-491.
Okai, K.H., Kanbara, K., Amano, k., Hagiwara, A., Sugita, C., Matsumoto, N.,Okai, Y., 2004. Potent Antioxidative and Antigenotoxic Activity in Aqueous Extract of Japanese Rice Bran – Association with Peroxidase Activity. Phytother Res.18, 628-633.
Oh, C.H., Oh, S.H., 2004. Effects of Germinated Brown Rice Extracts with Enhanced Levels of GABA on Cancer Cell Proliferation and Apoptosis. J. Med. Food.7, 19-23.
Cohen, S.A., Michaud, D.P., 1993. Synthesis of fluorescent derivertizating reagent, 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate and its application for the analysis of hydrolysate amino acids via high performance liquid chromatography. Anal. Biochem. 211, 279-287.
Soiampornkul, R., Junnu, S., Kanyok, S., Liammongkolkul, S., Katanyoo, W., Umpornsiriratm, S., 2012.Antioxidative and Neuroprotective activities of the Pre-Germinated Brown Rice extract. Food and Nutrition Science.3, 135-140.
Wang, H., Joseph, J.A., 1999. Quantifying Cellular Oxidative Stress by Dichlorofluorescein Assay Using Microplate Reader. Free Radical Biology & Medicine.27, 612-616.
Bicknell, G.R., Snowden, R.T., Cohen, G.M., 1994. Formation of high molecular mass DNA fragments is a marker of apoptosis in the human leukemic cell line, U937. Journal of Cell Science.107, 2483-2489.
Coyle, J.T., Puttfarcken, P., 1993.Oxidative stress. Science.262, 689-695.
Degterev, A., Boyce, M., Yuan, J., 2003. A decade of caspases. Oncogene. 22, 8543–8567.
Elmore, S., 2007. Apoptosis: A Review of Programmed Cell Death.ToxicolPathol. 35, 495–516.
Hockenbery, D.M., Oltvai, Z.N., Yin, X.M., Milliman, C.L., Korsmeyer, S.J., 1993. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell. 75, 241-251.
Yan, J.J., Cho, J.Y., Kim, H.S., Kim, K.L., Jung, J.S., Huh, S.O., Suh, H.W., Kim, Y.H., Song, D.K., 2001. Protection against -amyloid peptide toxicity in vivo with long-term administration of ferulic acid. Br. J.Pharmacol. 133,89-96.
Schubert, D., Behl, C,, Lesley, R., Brack, A.,Dargusch, R., Sagara, Y., Kimura, H.,1995 Amyloid peptides are toxic via a common oxidative mechanism. Proc. Natl. Acad. Sci. USA.92, 1989-93.
Scott, B.C., Aruoma, O.I., Evans, P.J., et al., 1994.Lipoic and dihydrolipoic acids as antioxidants. A critical evaluation. Free Radic. Res.20, 119-133.
Leventhal, A.G., Wang, Y., Pu, M., Zhou, Y., Ma, Y., 2003.GABA and its agonists improved visual cortical function in senescent monkeys. Science. 300:812-5.
Yang, F., Basu, T.K.,Ooraikul, B., 2001. Studies on Germination Conditions and Antioxidant Contents of Wheat Grain.Int. J. Food. Sci. Nut.52, 319-330.
Andarwulan, N., Fardiaz, D., Wattimena, G.A.,Shetty, K., 1999. Antioxidant Activity Associated with Lipid and Phenolic Mobilization during Seed Germination of Pangium edule Reinw.J. Agric. Food Chem. 47, 3158-3163.
Tian, S., Nakamura, K.,Kayahara, H., 2004. Analysis of Phenolic Compounds in White Rice, Brown Rice, and Germinated Brown Rice. J. Agric.Food Chem.52, 4808-4813.