Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test
International Journal of Nutrition and Food Sciences
Volume 2, Issue 6, November 2013, Pages: 312-319
Received: Nov. 4, 2013; Published: Nov. 30, 2013
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Authors
Farahnaaz Feroz, Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Jessica Das Senjuti, Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
Rashed Noor, Dept. of Microbiology, Stamford University Bangladesh, Dhaka, Bangladesh
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Abstract
Current study attempted to examine the growth and subsequent survival of the common spoilage bacteria in vegetable samples collected from Dhaka, Bangladesh. Carrot, cucumber, tomato and lettuce samples were obtained from local markets and rendered free of contaminants. Each sample was then inoculated separately with an array of 9 test bacteria, resulting in the initial load of 105 cfu/g. The results revealed more than 6-log reduction of Salmonella spp. in carrot and tomato samples, Shigella spp. in carrot, lettuce and cucumber samples, Aeromonas spp. in tomato samples, Pseudomonas spp. in lettuce samples, and Listeria spp. in cucumber samples. No significant reduction in E. coli was observed in the cucumber samples, while in carrots and tomato samples, approximately 2- log reductions was found. Demonstration of the capacity of vegetables to influence microbial growth would further aid in the maintenance of the food quality and stability as well as their shelf life. Conducting such experiments after the quantification of spoiling microorganisms thus imparts a complete bacteriological profile, which is of public health significance.
Keywords
Vegetables, Microorganisms, Growth, Challenge Test, Consumer Safety
To cite this article
Farahnaaz Feroz, Jessica Das Senjuti, Rashed Noor, Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test, International Journal of Nutrition and Food Sciences. Vol. 2, No. 6, 2013, pp. 312-319. doi: 10.11648/j.ijnfs.20130206.18
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