International Journal of Nutrition and Food Sciences

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A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array

Received: 25 November 2013    Accepted:     Published: 20 January 2014
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Abstract

E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.

DOI 10.11648/j.ijnfs.20140302.11
Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 2, March 2014)
Page(s) 14-18
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Escherichia coli O157, Peptide Nucleic Acid Array, Food Poisoning

References
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Author Information
  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Panagene Incorporated, Daejon 305-510, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Animal, Plant Quarantine Agency, Seoul regional office, Seoul 157-843, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

  • Animal, Plant Quarantine Agency, Anyang, Gyeonggido 430-824, South Korea

Cite This Article
  • APA Style

    Ki-Eun Lee, Woo-Seog Jeong, Seong-In Lim, Se-Ryun Kim, Yong-Kuk Kwon, et al. (2014). A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. International Journal of Nutrition and Food Sciences, 3(2), 14-18. https://doi.org/10.11648/j.ijnfs.20140302.11

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    ACS Style

    Ki-Eun Lee; Woo-Seog Jeong; Seong-In Lim; Se-Ryun Kim; Yong-Kuk Kwon, et al. A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. Int. J. Nutr. Food Sci. 2014, 3(2), 14-18. doi: 10.11648/j.ijnfs.20140302.11

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    AMA Style

    Ki-Eun Lee, Woo-Seog Jeong, Seong-In Lim, Se-Ryun Kim, Yong-Kuk Kwon, et al. A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. Int J Nutr Food Sci. 2014;3(2):14-18. doi: 10.11648/j.ijnfs.20140302.11

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  • @article{10.11648/j.ijnfs.20140302.11,
      author = {Ki-Eun Lee and Woo-Seog Jeong and Seong-In Lim and Se-Ryun Kim and Yong-Kuk Kwon and Jin-San Moon and Soo-Yeon Cho and Jae-Young Song and Dong-Jun An},
      title = {A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {3},
      number = {2},
      pages = {14-18},
      doi = {10.11648/j.ijnfs.20140302.11},
      url = {https://doi.org/10.11648/j.ijnfs.20140302.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20140302.11},
      abstract = {E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.},
     year = {2014}
    }
    

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  • TY  - JOUR
    T1  - A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array
    AU  - Ki-Eun Lee
    AU  - Woo-Seog Jeong
    AU  - Seong-In Lim
    AU  - Se-Ryun Kim
    AU  - Yong-Kuk Kwon
    AU  - Jin-San Moon
    AU  - Soo-Yeon Cho
    AU  - Jae-Young Song
    AU  - Dong-Jun An
    Y1  - 2014/01/20
    PY  - 2014
    N1  - https://doi.org/10.11648/j.ijnfs.20140302.11
    DO  - 10.11648/j.ijnfs.20140302.11
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 14
    EP  - 18
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20140302.11
    AB  - E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.
    VL  - 3
    IS  - 2
    ER  - 

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