Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction
International Journal of Nutrition and Food Sciences
Volume 3, Issue 3, May 2014, Pages: 141-144
Accepted: Apr. 20, 2014; Published: Apr. 30, 2014
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Authors
Asim A. Osman, Physiology Department, Faculty of Medicine, Zawia University, Libya; Physiology Department, Faculty of Medicine, GadarifUniversity, Sudan
Ayman M. Mahmoud, Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Egypt
Ayman S. Soliman, Physiology Department, Faculty of Medicine, Beni-Suef University, Egypt
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Abstract
This study was carried out to evaluatea PCR-based method for detection of DNA in cow milk. It utilized primers targeting the mitochondrial cytochrome-b (mtcyt-b) gene, which was used as a target DNA for PCR amplification. For the specific identification of cow mtcyt-b gene, a pair of primers (CSL1, CSR2), which produced a 386 bp PCR product from milk samples as well as from peripheral blood, were used. Amplification products were visualized on ethidium bromide-stained agarose gels. Amplification products were not detected when the PCR was applied to DNA from different animal species including sheep, camel, deerand human, indicating that the 2 pairs of primers are bovine specific. In conclusion, the PCR-based assay used in this study allowed sensitive and specific identification of cow milk DNA.
Keywords
Cytochrome-b, Cow Milk, PCR
To cite this article
Asim A. Osman, Ayman M. Mahmoud, Ayman S. Soliman, Rapid Detection of Bovine-Specific Nucleic Acid Sequences in Cow Milk Using Polymerase Chain Reaction, International Journal of Nutrition and Food Sciences. Vol. 3, No. 3, 2014, pp. 141-144. doi: 10.11648/j.ijnfs.20140303.12
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