Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian

doi:doi:10.11648/j.ijnfs.20140304.16

| Peer-Reviewed

Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)

Received: 5 June 2014 Accepted: 20 June 2014 Published: 30 June 2014

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.

Published in	International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)
DOI	10.11648/j.ijnfs.20140304.16
Page(s)	270-278
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Soft-shelled turtle Pelodiscus sinensis, Collagen extraction, Optimization, Characterization

References

[1]	Birk, D.E. and Bruckner, P. (2005). Collagen suprastructures. In Collagen. Springer Berlin Heidelberg. p. 185-205 .
[2]	Lee, C. H., Singla, A. & Lee, Y. (2001). Biomedical applications of collagen. International Journal of Pharmaceutics. 221, 1-22.
[3]	Gopinath, D., Ahmed, M.R., Gomathi, K., Chitra, K., Sehgal, P. K. & Jayakumar, R. (2004). Dermal wound healing processes with curcumin incorporated collagen films. Biomaterials. 25, 1911-1917.
[4]	Zeugolis, D.I., Paul, R.G. & Attenburrow, G. (2008). Extruded collagen-polyethylene glycol fibers for tissue engineering applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 85, 343-352.
[5]	Fernandez-Dıaz, M.D., Montero, P. & Gomez-Guillen, M.C. (2001). Gel properties of collagens from skins of cod (Gadus morhua) and hake (Merluccius merluccius) and their modification by the coenhancers magnesium sulphate, glycerol and transglutaminase. Food Chemistry. 74, 161-167.
[6]	Trevitt, C.R. & Singh, P.N. (2003). Variant Creutzfeldt-Jakob disease: pathology, epidemiology, and public health implications. The American Journal of Clinical Nutrition. 78, 651S-656S.
[7]	Abedin, M.Z., Karim, A.A., Ahmed, F., Latiff , A.A, Gan, C.Y., Ghazali, F.C. and Sarker, M.Z.I, Isolation and characterization of pepsin-solubilized collagen from the integument of sea cucumber (Stichopus vastus). Journal of the Science of Food and Agriculture. 93: 1083-1088 (2013).
[8]	Muralidharan, N., Shakila, R. J., Sukumar, D. & Jeyasekaran, G. (2013). Skin, bone and muscle collagen extraction from the trash fish, leather jacket (Odonus niger) and their characterization. Journal of Food Science and Technology. 50, 1106-1113.
[9]	Nalinanon, S., Benjakul, S. and Kishimura, H., (2010) Collagens from the skin of arabesque greenling (Pleurogrammus azonus) solubilized with the aid of acetic acid and pepsin from albacore tuna (Thunnus alalunga) stomach. Journal of the Science of Food and Agriculture. 90, 1492-1500.
[10]	Zhang, B., Chen, Y., Wei, X., Li, M. & Wang, M. (2010). Optimization of conditions for collagen extraction from the swim bladders of grass carp (Ctenopharyngodon idella) by response surface methodology. International Journal of Food Engineering. 6, 1-20.
[11]	Ministry of Agriculture and Fisheries Bureau of China, China Fishery Statistical Yearbook. China Agriculture Press. Beijing, China, pp. 41 (2011). (In Chinese)
[12]	Nagai, N., Kobayashi, H., Katayama, S. & Munekata, M. (2009). Preparation and characterization of collagen from soft-shelled turtle (Pelodiscus Sinensis) skin for biomaterial applications. Journal of Biomaterials Science, Polymer Edition. 20, 567-576.
[13]	Lu, J.F., Wan, Q., Yin, Z.M., Lin, L., Weng, S.B., Ye, Y.W. & Jiang, S.T. (2010). Extraction and characterization of collagen from calipash of Chinese soft-shelled turtle (Pelodiscus sinensis). Journal of Fisheries of China. 34, 981-988. (In Chinese with English abstract)
[14]	Tian, Y.T., Shen, H.H., Song, W., Mao, Y.T., Zhou, Y.R., Peng, X., Li, C.Y. & Qian, G.Y. (2013). Extraction technical optimization and characterization of collagen from Chinese soft-shelled turtle (Pelodiscus sinensis). JiangSu Agricultural Sciences. 41, 265-269. (In Chinese with English abstract).
[15]	Horwitz, W. (2000). Official methods of analysis of the Association of Official Analytical Chemists International. Recovery studies, 17th edn. Byrd Richmond, VA.
[16]	Li, Z.R., Wang, B., Chi, C.F., Zhang, Q.H., Gong, Y.D., Tang, J.J. & Ding, G.F. (2013). Isolation and characterization of acid soluble collagens and pepsin soluble collagens from the skin and bone of Spanish mackerel (Scomberomorous niphonius). Food Hydrocolloids. 31, 103-113.
[17]	ISO. (1978). Meat and meat products—Determination of L(2)-hydroxyproline content (Reference method). International Organization for Standarization, 3496(E).
[18]	Chen, S.J., Zeng, M.Y. & Dong, S.Y. (2004). Progress in the Study of Collagen and Active Peptide of Fisheries. Fisheries Science. 23, 44-46. (In Chinese with English abstract)
[19]	Zhu, F.J. (2012). Study on abalone visceral collagen peptide preparation and extracorporeal antioxidant acitivity. Master Dissertation, Fujian Agriculture and Forestry University, FuZhou. (In Chinese with English abstract).
[20]	Benjakul, S., Thiansilakul, Y., Visessanguan, W., Roytrakul, S., Kishimura, H., Prodpran, T. & Meesane, J. (2010). Extraction and characterisation of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus and Priacanthus macracanthus). Journal of the Science of Food and Agriculture. 90, 132-138.
[21]	Damrongsakkul, S., Ratanathammapan, K., Komolpis, K. & Tanthapanichakoon, W. (2008). Enzymatic hydrolysis of rawhide using papain and neutrase. Journal of Industrial and Engineering Chemistry. 14, 202-206.
[22]	Yin, S.W., Tang, C.H., Cao, J.S., Hu, E.K., Wen, Q.B., & Yang, X.Q. (2008). Effects of limited enzymatic hydrolysis with trypsin on the functional properties of hemp (Cannabis sativaL.) protein isolate. Food Chemistry. 106, 1004-1013.
[23]	Gunasekaran, S. (1998). U.S. Patent No. 5,814,328. Washington, DC: U.S. Patent and Trademark Office.
[24]	Li, X.Y. (2007). Study on extraction and the physical and chemical properties of pork lung collagen. Master dissertation, Southwest University, Chong Qing. (In Chinese with English abstract)
[25]	Jongjareonrak, A., Benjakul, S., Visessanguan, W. & Tanaka, M. (2005). Isolation and characterization of collagen from bigeye snapper (Priacanthus macracanthus) skin. Journal of the Science of Food and Agriculture. 85, 1203-1210.
[26]	Aukkanit, N. & Garnjanagoonchorn, W. (2010). Temperature effects on type I pepsin-solubilised collagen extraction from silver-line grunt skin and its in vitro fibril self-assembly. Journal of the Science of Food and Agriculture. 90, 2627-2632.
[27]	Shahidi, F., Naczk, M., Pegg, R.B. & Synowiecki, J. (1991). Chemical composition and nutritional value of processing discards of cod (Gadus morhua). Food Chemistry. 42, 145-151.
[28]	Holye, N.T. & Merritt, J.O.H.N. (1994). Quality of fish protein hydrolysates from herring (Clupea harengus). Journal of Food Science. 59, 76-79.
[29]	Cui, F.X., Xue, C.H., Li, Z.J., Dong, P. and Xue, D.M. (2006)Extraction and the physical and chemical characterization of collagen in Stichopus japonicus. J Fish China. 30, 549-553. (In Chinese with English abstract)
[30]	Lin, Y.K. & Liu, D.C. (2006). Comparison of physical–chemical properties of type I collagen from different species. Food Chemistry. 99, 244-251.
[31]	Zhu, B.W., Dong, X.P., Zhou, D.Y., Gao, Y., Yang, J.F., Li, D.M. & Yu, C. (2012). Physicochemical properties and radical scavenging capacities of pepsin-solubilized collagen from sea cucumber Stichopus japonicus. Food Hydrocolloids. 28, 182-188.
[32]	Woo, J.W., Yu, S.J., Cho, S.M., Lee, Y.B. & Kim, S.B. (2008). Extraction optimization and properties of collagen from yellowfin tuna (Thunnus albacares) dorsal skin. Food Hydrocolloids. 22, 879-887.
[33]	Wang, L., An, X.X., Yang, F., Xin, Z., Zhao, L. & Hu, Q. (2008). Isolation and characterisation of collagens from the skin, scale and bone of deep-sea redfish (Sebastes mentella). Food Chemistry. 108, 616-623.
[34]	Muyonga, J.H., Cole, C.G.B. & Duodu, K.G. (2004). Characterisation of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food Chemistry. 85, 81-89.
[35]	Ikoma, T., Kobayashi, H., Tanaka, J., Walsh, D. & Mann, S. (2003). Physical properties of type I collagen extracted from fish scales of Pagrus major and Oreochromis niloticas. International Journal of Biological Macromolecules. 32, 199-204.
[36]	Wang, L., Liang, Q.F., Chen, T.T., Wang, Z.B., Xu, J.M. & Ma, H. (2014). Characterization of collagen from the skin of Amur sturgeon (Acipenser schrenckii). Food Hydrocolloids. 38, 104-109.
[37]	Burjanadze, T.V. (2000). New analysis of the phylogenetic change of collagen thermostability. Biopolymers. 53, 523-528.
[38]	Josse, J. & Harrington, W.F. (1964). Role of pyrrolidine residues in the structure and stabilization of collagen. Journal of Molecular Biology. 9, 269-287.
[39]	Ahmad, M. & Benjakul, S. (2010). Extraction and characterisation of pepsin-solubilised collagen from the skin of unicorn leatherjacket (Aluterus monocerous). Food Chemistry. 120, 817-824.
[40]	Angele, P., Abke, J., Kujat, R., Faltermeier, H., Schumann, D., Nerlich, M. & Mueller, R. (2004). Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices. Biomaterials. 25, 2831-2841.

Cite This Article

Plain Text BibTeX RIS

APA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. (2014). Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. International Journal of Nutrition and Food Sciences, 3(4), 270-278. https://doi.org/10.11648/j.ijnfs.20140304.16

Copy | Download

ACS Style

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int. J. Nutr. Food Sci. 2014, 3(4), 270-278. doi: 10.11648/j.ijnfs.20140304.16

Copy | Download

AMA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int J Nutr Food Sci. 2014;3(4):270-278. doi: 10.11648/j.ijnfs.20140304.16

Copy | Download

@article{10.11648/j.ijnfs.20140304.16,
  author = {Wei Song and Wei Chen and Ya’nan Yang and Caiyan Li and Guoying Qian},
  title = {Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {270-278},
  doi = {10.11648/j.ijnfs.20140304.16},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.16},
  abstract = {The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis
AU  - Wei Song
AU  - Wei Chen
AU  - Ya’nan Yang
AU  - Caiyan Li
AU  - Guoying Qian
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.16
DO  - 10.11648/j.ijnfs.20140304.16
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 270
EP  - 278
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.16
AB  - The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.
VL  - 3
IS  - 4
ER  -

Copy | Download

Author Information

Wei Song

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Wei Chen

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Ya’nan Yang

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Caiyan Li

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China
Guoying Qian

College of Biological and Environmental Sciences, Zhejiang Wanli University, South Qianhu Road 8, Ningbo 315100, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. (2014). Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. International Journal of Nutrition and Food Sciences, 3(4), 270-278. https://doi.org/10.11648/j.ijnfs.20140304.16

Copy | Download

ACS Style

Wei Song; Wei Chen; Ya’nan Yang; Caiyan Li; Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int. J. Nutr. Food Sci. 2014, 3(4), 270-278. doi: 10.11648/j.ijnfs.20140304.16

Copy | Download

AMA Style

Wei Song, Wei Chen, Ya’nan Yang, Caiyan Li, Guoying Qian. Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis. Int J Nutr Food Sci. 2014;3(4):270-278. doi: 10.11648/j.ijnfs.20140304.16

Copy | Download

@article{10.11648/j.ijnfs.20140304.16,
  author = {Wei Song and Wei Chen and Ya’nan Yang and Caiyan Li and Guoying Qian},
  title = {Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {270-278},
  doi = {10.11648/j.ijnfs.20140304.16},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.16},
  abstract = {The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Extraction Optimization and Characterization of Collagen from the Lung of Soft-Shelled Turtle Pelodiscus Sinensis
AU  - Wei Song
AU  - Wei Chen
AU  - Ya’nan Yang
AU  - Caiyan Li
AU  - Guoying Qian
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.16
DO  - 10.11648/j.ijnfs.20140304.16
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 270
EP  - 278
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.16
AB  - The soft-shelled turtle (Pelodiscus sinensis) is a commercially important aquatic species with abundant collagen content and precious nutritious high quality goods. The present study was to optimize the collagen extraction from lung of Pelodiscus sinensis. Single-factor test was employed to investigate the effects of different extraction methods and the major factors that influence the collagen production in enzymatic extraction method on the collagen yield of lung. Optimization of the papain enzymatic extraction parameters was then examined using an orthogonal test design L9 (34). The optimum extraction conditions were obtained when the extraction temperature, papain enzyme dose, the ratio of solid to solution, and extraction time were 32°C, 4.0mg/ml, 1:35, and 12h, respectively. Under the optimized conditions, the collagen yield was up to 79.29%. The properties of turtle lung collagen were characterized by SDS-PAGE, UV scanning, and amino acid analysis. The results showed that the extracted lung collagen had high imino acid content at 21.8% and contained two α chains, β, and γ-components, belonging to the typical type I collagen. The amphibious aquatic turtle collagen is thought to be a viable novel source for replacement of terrestrial mammals and could provide scientific reference for the development of collagen polypeptide for terrapin animals.
VL  - 3
IS  - 4
ER  -

Copy | Download