Nutritional Study of Some Food Coloring Agents on Experimental Rats
International Journal of Nutrition and Food Sciences
Volume 3, Issue 6, November 2014, Pages: 538-544
Received: Sep. 23, 2014; Accepted: Oct. 20, 2014; Published: Nov. 20, 2014
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Authors
Wagih Ahmad El-Malky, Division of Medical Foods, National Organization for Drug Control and Research (NODCAR), 6-7, AboHazem St, Pyramids, Giza, Egypt
Ghada Mahmoud Khiralla, Division of Medical Foods, National Organization for Drug Control and Research (NODCAR), 6-7, AboHazem St, Pyramids, Giza, Egypt
Safaa Ahmad Salem, Division of Medical Foods, National Organization for Drug Control and Research (NODCAR), 6-7, AboHazem St, Pyramids, Giza, Egypt
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Abstract
This study was designed to recognize the biochemical and histological alterations in the liver and kidney of male rats due to daily oral intake (for 8 weeks) of beet and curcumin extracts as natural red and yellow color, edicol erythrosine and sunset yellow as recommended synthetic colors and two unknown commercial coloring agents (red and yellow). The effect of admissible daily intake (ADI) and overdose (5 times) of the synthetic recommended and commercial colors was also investigated. Compared with the control group, the GBW % of rats indicated significant (p<0.05) increase in all studied groups except that given the natural beet extract and that given ADI of edicol erythrosine. No significant differences were detected in the relative kidney weights between groups. Rats that administrated overdose of the studied synthetic colors showed significant increase in the lipid profile of blood including total cholesterol, low density lipoproteins (LDL), high density lipoproteins (HDL). The obtained histopathological changes in liver and kidney of rats administrated overdose of colorants were associated with elevation of urea, creatinine, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), which were strictly related to the injury of kidney and liver functions. The present study demonstrated that beet extract could be used as alternative natural red colorant, whereas curcumin extract need additional long-term studies because of the elevation in urea and AST levels in addition to massive aggregative of inflammatory cells infiltration in the portal area of liver of rats administrated the 7.87 mg curcumin /Kg BW. Also, the present study sheds light on the nutritional hazards in the liver and kidney due to the uncontrolled use of synthetic color.
Keywords
Beet, Curcumin, Liver, Kidney, Erythrosine, Sunset Yellow
To cite this article
Wagih Ahmad El-Malky, Ghada Mahmoud Khiralla, Safaa Ahmad Salem, Nutritional Study of Some Food Coloring Agents on Experimental Rats, International Journal of Nutrition and Food Sciences. Vol. 3, No. 6, 2014, pp. 538-544. doi: 10.11648/j.ijnfs.20140306.18
References
[1]
Helal, E.G.E., S.A.M. Zaahkouk, and H.A. Mekkawy, Effect of some food colorants (synthetic and natiral products) of young albino rats. Egypt. J. Hospital Med., 2000. 1: p. 103-113.
[2]
Soltan, S.S.A. and M.M.E.M. Shehata, The effects of using color foods of children on immunity properties and liver, kidney on rats. Food and Nutrition Sciences, 2012. 3: p. 897-904.
[3]
Salah, S.H., Biochemical studies on some synthetic food colorants. M.Sc. (Blochemistry). Fac. Agric., Cairo Univ. 1994.
[4]
Ganong, W.E., Review of medical physiology. 15th ed. Lange Medical Book. 1991.
[5]
Hashem, M.M., et al., Immunological studies on amaranth sun-set yellow and curcumin as food coloring agents in albino rats. Food and Chemical Toxicology, 2010. 48(6): p. 1581-1586.
[6]
Lakdawalla, A.A. and M.S. Netrawali, Mutagenicity, comutagenicity, and antimutagenicity of erythrosine (FD and C Red 3), a food dye, in the Ames/Salmonella assay. Mutation Research/Genetic Toxicology, 1988. 204(2): p. 131-139.
[7]
Hassan, G.M., Effect of some synthetic coloring additives on dna damage and chromosomal aberration of rats. Arab Journal. Biotechnology, 2009. 13(1): p. 13-24.
[8]
(EFSA) European Food Safety Authority, Scientific Opinion on the re-evaluation of Erythrosine (E 127) as a food additive. EFSA Journal, 2011. 9(1): p. 1854.
[9]
Socaciu, C., Natural pigments as food colorants. In: Socaciu C, editor. Food colorants: chemical and functional properties. Boca Raton, Fla.: CRC Press. ph. 583-601. 2008.
[10]
Dabas, D., et al., A Colored Avocado Seed Extract as a Potential Natural Colorant. Journal of Food Science, 2011. 76(9): p. C1335-C1341.
[11]
Waschulewski, I.H. and R.A. Sunde, Effect of dietary methionine on utilization of tissue selenium from dietar selenomethionine for glutathione peroxidase in the rat. J. Nutr., 1988. 118: p. 367-374.
[12]
Lowry, O.H., et al., Protein measurement with the Folin Phenol Reagent. J. Bio. Chem., 1951. 193: p. 269-275.
[13]
Patton, C.J. and S.R. Crouch, Spectrophotometric and kinetic investigation of the bertha lot reaction for the determination of ammonia. Anal. Chem., 1977. 49: p. 464-469.
[14]
Faulkner, W.R. and J.W. King, Fundamentals of Clinical Chemistry, 1st ed., The Alden Press and Kemp Hall Bindery, Oxford, Britain. 1976.
[15]
Bergmeyer, H.U. and M. Harder, A colorimeteric methods of determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Clin. Biochem., 1986. 24: p. 28 - 34.
[16]
Banchroft, J.D., A. Stevens, and D.R. Turner, Theory and practice of histological techniques. 4 ed. 1996: Churchil Livingstone, New York, London, San Francisco, Tokyo.
[17]
Borzelleca, J.F. and J.B. Hallagan, Multigeneration study of FD & C Red No. 3 (erythrosine) in Sprague-Dawley rats. Food Chem Toxicol. , 1990. 28(12): p. 813-819.
[18]
Christianson, D., et al., The sex-related difference in serum thyrotropin concentration is androgen mediated. Endocrinology, 1981. 108: p. 529-535.
[19]
Sarhan, M.A.A., A.A. Shati, and F.G. Elsaid, Biochemical and molecular studies on the possible influence of the Brassica oleracea and Beta vulgaris extracts to mitigate the effect of food preservatives and food chemical colorants on albino rats. Saudi Journal of Biological Sciences, 2014. 21(4): p. 342-354.
[20]
Wang, S., B. Chen, and C. Sun, Regulation effect of curcumin on blood lipids and antioxidation in hyperlipidemia rats. Wei Sheng Yan Jiu., 2000. 29(4): p. 240-242.
[21]
Fan, C., et al., Effect of curcumin on the expression of LDL receptor in mouse macrophages. Journal of Ethnopharmacology, 2006. 105(1–2): p. 251-254.
[22]
Rhiouania, H., et al., Acute and subchronic toxicity of an aqueous extract of the leaves of Herniaria glabra in rodents. Journal of Ethnopharmacology, 2008. 118: p. 378-386.
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