International Journal of Nutrition and Food Sciences

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Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes

Received: 19 January 2015    Accepted: 01 February 2015    Published: 09 February 2015
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Abstract

In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.

DOI 10.11648/j.ijnfs.20150401.20
Published in International Journal of Nutrition and Food Sciences (Volume 4, Issue 1, January 2015)
Page(s) 69-74
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lipolytic Effect, Eumicrotremus Orbis, 3T3-L1 Adipocytes, Anti-Obesity, Marine Resource

References
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Author Information
  • Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea; Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea

  • Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea

  • Jeonbuk Institute for Bioindustry, Jeonju, Korea

  • Gi-jang Mulsan Col., Busan, Korea

  • Division of Marine Food Bioscience & Technology, Gangneung National University, Gangneung, Korea

  • Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea

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  • APA Style

    Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. (2015). Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. International Journal of Nutrition and Food Sciences, 4(1), 69-74. https://doi.org/10.11648/j.ijnfs.20150401.20

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    ACS Style

    Byoung-Mok Kim; Heang-Jeun Heo; Dong-Soo Kim; Young-Myung Kim; In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int. J. Nutr. Food Sci. 2015, 4(1), 69-74. doi: 10.11648/j.ijnfs.20150401.20

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    AMA Style

    Byoung-Mok Kim, Heang-Jeun Heo, Dong-Soo Kim, Young-Myung Kim, In-Hack Jeong, et al. Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes. Int J Nutr Food Sci. 2015;4(1):69-74. doi: 10.11648/j.ijnfs.20150401.20

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  • @article{10.11648/j.ijnfs.20150401.20,
      author = {Byoung-Mok Kim and Heang-Jeun Heo and Dong-Soo Kim and Young-Myung Kim and In-Hack Jeong and Young-Min Chi},
      title = {Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {4},
      number = {1},
      pages = {69-74},
      doi = {10.11648/j.ijnfs.20150401.20},
      url = {https://doi.org/10.11648/j.ijnfs.20150401.20},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20150401.20},
      abstract = {In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes
    AU  - Byoung-Mok Kim
    AU  - Heang-Jeun Heo
    AU  - Dong-Soo Kim
    AU  - Young-Myung Kim
    AU  - In-Hack Jeong
    AU  - Young-Min Chi
    Y1  - 2015/02/09
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ijnfs.20150401.20
    DO  - 10.11648/j.ijnfs.20150401.20
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 69
    EP  - 74
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20150401.20
    AB  - In this study, we investigated the effects of Eumicrotremus orbis water extracts (EOWE) on lipolysis in differentiatied 3T3-L1 adipocytes and its mechanism. It was observed that there was no cytotoxicity for adipocytes of EOWE. The content of glycerol released in cell culture medium increased dependent on sample concentration. In differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by approximately 3.43 and 1.77 times as compared to the controls. But, adipose triglyceride lipase (ATGL) protein level did not effect as compared to the controls. Treated EOWE inhibited the protein expression of perilipin A. In other word, it is judged to be due to, a content of glycerol released in cell culture medium increased through not only increase of HSL, p-HSL and p-PKA protein level, but inhibit of perilipin A. Therefore, our results suggest that EOWE may induce lipoylsis by increasing the protein levels of HSL, p-HSL and p-PKA.
    VL  - 4
    IS  - 1
    ER  - 

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