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Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat

Received: 7 July 2015    Accepted: 13 July 2015    Published: 28 July 2015
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Abstract

This study aimed to evaluate the effect of feeding rats on virgin olive oil (VOO) and extra virgin olive oil (EVOO) with or without thyme powder on health status of their first generation. Outcome of pregnancy in each group (pregnant and lactating rats fed on olive oils and thyme which randomly divided into 15 groups) were divided into 2 subgroups (male and female) and feeding a month later (till 8 weeks aged). The babies were weighted, blood was collected and scarified. Organs were removed and weighted. Biochemical assays and bone measurements were evaluated. Generally, the groups fed on VOO had a high number of babies, a low body weight and high of liver weight compared to groups fed on EVOO. Total cholesterol (TC), LDL-C and risk factor of male fed on olive oils (100%) had higher values than groups fed on olive oils (50%). Female groups showed observed that, which groups fed on olive oils (100%) had higher of TC, LDL-C and risk factor than groups fed on (50%). Creatinine and urea were increased in male rats fed on olive oils while decreased in the female rats compared to control group. Male groups fed on VOO and thyme were higher of AST, ALT and ALP than groups fed on EVOO. Concerning female babies, there are a high differences between groups. In groups (male and female) were high levels of Ca and P content of bone were observed in rats fed on VOO and thyme leaves powder compared to groups fed on EVOO and thyme powder diets. In conclusion, the feeding on olive oils (VOO and EVOO) with or without thyme leaves powder diets caused to increasing of fertility, improved lipid profile and increasing Ca and P of bone contents.

Published in International Journal of Nutrition and Food Sciences (Volume 4, Issue 5)
DOI 10.11648/j.ijnfs.20150405.11
Page(s) 509-517
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Virgin Olive Oil, Extra Virgin Olive Oil, Thyme Leaves Powder, Rats, Biochemical Assays, Bone Measurements

References
[1] Siemelink, M; Verhoef, A; Dormans, JAM and Span, PN (2002).Dietary fatty acid composition during pregnancy and lactation in the rat programs growth and glucose metabolism in offspring. Diabetologia, 45, 1397 - 1403.
[2] Vickers, MH; Krechowec, SO and Breier, BH (2007).Is later obesity programmed in utero? Curr. Drug Targets, 8(8): 923 - 934.
[3] Hill, DJ and Duvillie, B (2000). Pancreatic development and adult diabetes.Pediatr Res, 48(3): 269 - 274.
[4] Armitage, JA; Taylor, PD and Poston L (2005). Experimental models of developmental programming consequences of exposure to an energy rich diet during development.J Physiol, 565.1 pp. 3 - 8.
[5] Gillman, MW; Rifas-Shiman, SL; Kleinman, K; Oken, E; Rich-Edwards, JW and Taveras, EM (2008). Developmental origins of childhood overweight: Potential Public Health Impact. Obesity (Silver Spring), 16 (7): 1651- 1656.
[6] Omeni, AA; Otamere, HO; Adisa, WA; Osifo, UC; Iyamu, OA; Ediangbe, AP; Akpamu, U and Odiase, DE (2012). A Comparative study on the role of endothelium in aortic vascular response to phenylephrine in normal anddiabetic pregnancy. European Journal of Experimental Biology, 2(6): 1974 - 1979.
[7] Howie, GJ; Sloboda, DM; Kamal, T and Vickers, MH (2009).Maternal nutritional history predicts obesity in adult offspring independent of postnatal diet. J.Physiol, 587, (4), pp. 905 – 915.
[8] Moldao-Martins, M; Beirao-da-Costa, S; Neves, C; Cavaleiro, C; Salgueiro, L and Beirao-da-Costa, ML (2004). Olive oil flavored by the essential oils of Mentha x piprita and thymus mastichina L. Food Quality and Preference 15, 447 – 452.
[9] Kok FJ and Kromhout D (2004). Atherosclerosis, epidemiological studies on the health effects of a Mediterranean diet. Eur J Nutr.; 43: 2-5.
[10] Covas, MI (2007). Olive oil and cardiovascular system.Pharmacol. Res., 55; 175–186.
[11] Youdim, K.A. and Deans, S.G. (1999). Dietary supplementation of thyme (thymus vulgaris L) essential oil during the life time of the rats its effects on the antioxidant status, in liver, kidney and heart tissues. Mechanisms of Aging and Development. 109:163-175.
[12] Tsimidou, M; Papavergou, E and Boskou, D (1995). Evaluation of oregano antioxidant in mackerel oil. Food Research International 28, 431 – 433.
[13] Ayadi, MA; Grati-Kamoun, N and Attia, H (2009). Physico-chemical change and heat stability of extra virgin olive oils flavored by selected Tunisian aromatic plants. Food and Chemical Toxicology, 47; 2613 – 2619.
[14] El-Ghousein, S.S. and Al-Beitowi, N.A. (2009). The effect of feeding crushed thyme (Thymus vulgaris L) on growth, blood constituents and gastrointestinal tract and carcass characteristics of broiler chicken. J. Poultry Assoc. 46:100-104.
[15] Reeves, P.G.; Nielsen, F.H. and Fahey, G.C. (1993). AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad HOC writing Committee on the reformulation of the AIN-76 a rodent diet. J Nutr., 123(12): 1939- 1951.
[16] Drury, RA and Wallington, EA (1980). Carton's. Histological Technique. 5thed Oxford Univ.
[17] Allain, CZ; Poon, ZS and Chen, CS (1974): Enzymatic determination of total serum cholesterol. Clin Chem. 20: 470 - 475.
[18] Fossati, P and Principle, L (1982). Enzymatic colorimetric method to determination triglyceriods. Clin. Chem. 28: 2077.
[19] Burstein, M (1970): HDL cholesterol determination after separation high density lipoprotein. Lipid. Res. 11: 583.
[20] Essam El-Din, Maha, M (2012). The protective effect of Turnip leaves against oxidative stress induced by high cholesterol diet in adult rats. World Applied Science Journal 20 (1): 154 – 163.
[21] Abbott, RD; Wilson, PW; Kannel, WB and Castelli, WP (1988). High density lipoprotein cholesterol, total cholesterol screening, and myocardial infarction. The Framingham Study. Arteriosclerosis, 8, 207–211.
[22] Patton, CJ and Crouch, SR (1977). Enzymatic colorimetric method to determination urea in serum. Anal. Chem. 49: 464.
[23] Bohmer, HBUM (1971): Micro determination of creatinine Clin. Chem. Acta 32: 81-85.
[24] Reitman, S and Frankel, S (1957). A colorimetric method for the determination of serum glutamic oxaloactic and glucanic pyruvic transaminase. Am. J. Clin. Path. 28: 56.
[25] Belfield, A and Golobkrg, DM (1971): Human serum glucose- 6- phosphate activity conformation of its presence and lack of diagnostic value. Enzyme 12: 561.
[26] AOAC (2000). Official Methods of Analysis of the Association of the Analytical Chemists. 17thed published by the Association of Official Analytical Chemists. Po Box 540. Benjamin Franklin Station Washington DC.
[27] Arjmand, BH; Alekel, L; Hollis, BW; Amin, D; Sapuntialcis, AS, Guo, P and Pukereja, SC (1996): Dietary soybean prevents bone loss in an ovariectomyed not model of osteoporosis. J. Nut. 126: 161-167.
[28] Snedecor, GW and Cochran WG (1989). Statistical methods. The Lowa State University Press. Ames, Lowe.
[29] Bernardi JR; Escobar R; Ferreira FC and Silveira PP (2012). Fetal and Neonatal Levels of Omega-3: Effects on Neurodevelopment, Nutrition, and Growth. The Scientific World Journal Vol. 2012. http://dx.doi.org/10.1100/2012/202473
[30] Salem, AA (2015). Effect of feeding on olive oil and thyme on pregnancy and lactation periods. Inter. J Nutr. Food Sci., 4(1): 19 – 28.
[31] Beynen, A.C., J.J. Visser, J.A. Schouten and M.B. Katan, 1987. Cholesterol metabolism in rabbits fed diets containing either corn oil or olive oil. Nutr. Rep. Intern., 35: 111-116.
[32] Hayes, K.C., A. Pronezuk, S. Lindsey and D. Diersen-Schade, 1994. Dietary saturated fatty acids (12:0, 14:0, 16:0) differ in their impact on plasma cholesterol and lipoproteins in non-human primates. Am. J. Clin. Nutr., 53: 491-498.
[33] Morello, J.R., M.J. Motilva, M.J. Tovar and M.P. Romero, 2007. Changes in commercial virgin olive oil during storage, with special emphasis on the phenolic fraction. J. Agric. Food Chem., 85: 357-364.
[34] Puela, C., A. Quintina, A. Agaliasa, J. Matheya, C. Obleda and A. Mazura, 2004. Olive oil and its main phenolic micronutrient (oleuropein) prevent inflammation induced bone loss in the ovariectomised rat. Br. J. Nutr., 92: 119-127.
[35] Mroueh, M; Sakr, J and Daher, C (2009). The effects of oil enriched diet on plasma lipid profile, glucose and coagulation time in rats. PAN Arab. Med. J., 1(4): 25-31.
[36] Al-Amoudi, NS and Abu Araki, HA (2013). Evaluation of vegetable and fish oils diets for the amelioration of diabetes side effects. Journal of Diabetes & Metabolic Disorders 12:13 - 19.
[37] Gorinstein, S; Leontowicz, HS;Leontowicz, M; Ciz, M; Krzeminski, R; Gralak, M.; Czerwinski, J; Jastrzebski, Z; Trakhtenberg, S; Grigelme-Mignel, N; Soliva –Fortung, R and Marten-Belloso, O (2002): Olive oil improve lipid metabolism and increase antioxidant potential in rats fed diet containing cholesterol. J. Agric. Food Chem. 50: 6102-6108.
[38] Aguilera CM; Ramirez-Tortosa MC; Mesa MD; Ramirez- Tortosa CL and Gil A (2002). Sunflower, virgin-olive and fish oils differentially affect the progression of aortic lesions in rabbits with experimental atherosclerosis. Atherosclerosis, 162: 335 – 344.
[39] Fitó M; Cladellas M; de la Torre R; Martí J; Alcántara M; Pujadas-Bastardes M; Marrugat J; Bruguera J; López-Sabater MC; Vila J; Covas MI and members of the SOLOS Investigators (2005). Antioxidant effect of virgin olive oil in patients with stable coronary heart disease: a randomized, crossover, controlled, clinical trial. Atherosclerosis; 181:149-58.
[40] Cicerale S; Conlan XA; Sinclair AJ and Keast RSJ (2009). Chemistry and health of olive oil phenolics. Critical Reviews in Food Science and Nutrition; 49: 218-36.
[41] Nandakumaran M; Al- Shammari M; Al-Sarraf H; Al-Dossery M and Al-Saleh E(2012). Effect of olive oil administration on some hematologic and metabolic parameters in female rats. Ind. J.Biol. Sci., 18: 1 – 8.
[42] Carajal-Zarrabal O; Nolasco-Hipolito C; Aguilar-Uscanga Ma.G; Santiesteban GM; Hayward-Jones PM and Barrada-Dermitz D Ma (2014). Effect of Dietary Intake of Avocado Oil and Olive Oil on Biochemical Markers of Liver Function in Sucrose-Fed Rats.BioMed Research International, Vol. 2014, 8 pages. http://dx.doi.org/10.1155/2014/595479
[43] Yang, ZH; Miyahara, H; Takeo, J and Katayama, M (2012). Diet high in fat and sucrose induces rapid onset of obesity-related metabolic syndrome partly through rapid response of genes involved in lipogenesis, insulin signaling and inflammation in mice. Diabetology & Metabolic Syndrome, vol. 4(1): 32 - 37.
[44] Rezq, AA; Labib, FA and Attia, AM (2010). Effect of Some Dietary Oils and Fats on Serum Lipid Profile, Calcium Absorption and Bone Mineralization in Mice. Pakistan Journal of Nutrition 9 (7): 643-650.
[45] Kotogionni, MD, Melistas, L; Yanakoulia, M.Malagaris I, Panagiotakos DB and Yiannakouris N (2009). Association between dietary patterns and indices of bone mass in a sample of Mediterranean women. Nutr. 25 (2): 165 - 171.
[46] Trichopoulou A; Georgiou E; Bassiakos Y; Lipworth L; Lagiou P; Proukakis C and Trichopoulos D (1997). Energy intake and monounsaturated fat in relation to bone mineral density among women and men in Greece. Prev. Med. 26: 395– 400.
[47] Puel C, Mathey J, Agalias A, Kati-Coulibaly S, Mardon J, Obled C, Davicco MJ, Lebecque P, Horcajada MN, Skaltsounis AL and Coxam V(2006). Doseresponse study of effect of oleuropein, an olive oil polyphenol, in an ovariectomy/inflammation experimental model of bone loss in the rat. Clin. Nutr. 25 (5): 859 – 868.
[48] Hagiwara, K; Goto, T; Araki, M; Miyazaki, H and Hagiwara, H (2011). Olive polyphenol hydroxytyrosol prevents bone loss. Euro. J. Pharmacol., 662: 78 – 84.
[49] Watkins BA, Li Y, Lippman HE and Seifert MF. Omega-3 polyunsaturatedfatty acids and skeletal health. Exp Biol Med 2001;226: 485–97.
[50] Trichopoulou, A., A. Naska and T. Costacou, 2002. Dafen III Group Disparities in food habits across Europe. Proc. Nutr. Soc., 61: 553-558.
Cite This Article
  • APA Style

    Amany A. Salem, Hayam A. Elsawy, Salwa S. Gabal. (2015). Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat. International Journal of Nutrition and Food Sciences, 4(5), 509-517. https://doi.org/10.11648/j.ijnfs.20150405.11

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    ACS Style

    Amany A. Salem; Hayam A. Elsawy; Salwa S. Gabal. Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat. Int. J. Nutr. Food Sci. 2015, 4(5), 509-517. doi: 10.11648/j.ijnfs.20150405.11

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    AMA Style

    Amany A. Salem, Hayam A. Elsawy, Salwa S. Gabal. Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat. Int J Nutr Food Sci. 2015;4(5):509-517. doi: 10.11648/j.ijnfs.20150405.11

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  • @article{10.11648/j.ijnfs.20150405.11,
      author = {Amany A. Salem and Hayam A. Elsawy and Salwa S. Gabal},
      title = {Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {4},
      number = {5},
      pages = {509-517},
      doi = {10.11648/j.ijnfs.20150405.11},
      url = {https://doi.org/10.11648/j.ijnfs.20150405.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20150405.11},
      abstract = {This study aimed to evaluate the effect of feeding rats on virgin olive oil (VOO) and extra virgin olive oil (EVOO) with or without thyme powder on health status of their first generation. Outcome of pregnancy in each group (pregnant and lactating rats fed on olive oils and thyme which randomly divided into 15 groups) were divided into 2 subgroups (male and female) and feeding a month later (till 8 weeks aged). The babies were weighted, blood was collected and scarified. Organs were removed and weighted. Biochemical assays and bone measurements were evaluated. Generally, the groups fed on VOO had a high number of babies, a low body weight and high of liver weight compared to groups fed on EVOO. Total cholesterol (TC), LDL-C and risk factor of male fed on olive oils (100%) had higher values than groups fed on olive oils (50%). Female groups showed observed that, which groups fed on olive oils (100%) had higher of TC, LDL-C and risk factor than groups fed on (50%). Creatinine and urea were increased in male rats fed on olive oils while decreased in the female rats compared to control group. Male groups fed on VOO and thyme were higher of AST, ALT and ALP than groups fed on EVOO. Concerning female babies, there are a high differences between groups. In groups (male and female) were high levels of Ca and P content of bone were observed in rats fed on VOO and thyme leaves powder compared to groups fed on EVOO and thyme powder diets. In conclusion, the feeding on olive oils (VOO and EVOO) with or without thyme leaves powder diets caused to increasing of fertility, improved lipid profile and increasing Ca and P of bone contents.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Impact of Feeding on Olive Oil with Thyme on Health Status of First Generation of Rat
    AU  - Amany A. Salem
    AU  - Hayam A. Elsawy
    AU  - Salwa S. Gabal
    Y1  - 2015/07/28
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ijnfs.20150405.11
    DO  - 10.11648/j.ijnfs.20150405.11
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 509
    EP  - 517
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20150405.11
    AB  - This study aimed to evaluate the effect of feeding rats on virgin olive oil (VOO) and extra virgin olive oil (EVOO) with or without thyme powder on health status of their first generation. Outcome of pregnancy in each group (pregnant and lactating rats fed on olive oils and thyme which randomly divided into 15 groups) were divided into 2 subgroups (male and female) and feeding a month later (till 8 weeks aged). The babies were weighted, blood was collected and scarified. Organs were removed and weighted. Biochemical assays and bone measurements were evaluated. Generally, the groups fed on VOO had a high number of babies, a low body weight and high of liver weight compared to groups fed on EVOO. Total cholesterol (TC), LDL-C and risk factor of male fed on olive oils (100%) had higher values than groups fed on olive oils (50%). Female groups showed observed that, which groups fed on olive oils (100%) had higher of TC, LDL-C and risk factor than groups fed on (50%). Creatinine and urea were increased in male rats fed on olive oils while decreased in the female rats compared to control group. Male groups fed on VOO and thyme were higher of AST, ALT and ALP than groups fed on EVOO. Concerning female babies, there are a high differences between groups. In groups (male and female) were high levels of Ca and P content of bone were observed in rats fed on VOO and thyme leaves powder compared to groups fed on EVOO and thyme powder diets. In conclusion, the feeding on olive oils (VOO and EVOO) with or without thyme leaves powder diets caused to increasing of fertility, improved lipid profile and increasing Ca and P of bone contents.
    VL  - 4
    IS  - 5
    ER  - 

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Author Information
  • Special Food and Nutrition Department, Food Technology Research Institute, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt

  • Special Food and Nutrition Department, Food Technology Research Institute, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt

  • Special Food and Nutrition Department, Food Technology Research Institute, Agriculture Research Center, Ministry of Agriculture, Giza, Egypt

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