Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats
International Journal of Nutrition and Food Sciences
Volume 5, Issue 5, September 2016, Pages: 310-317
Received: Jul. 3, 2016; Accepted: Jul. 13, 2016; Published: Aug. 4, 2016
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Reda Ahmed Mokhtar, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Nefisa Hassan El-Banna, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Mohammed Hamdy Haggag, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
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This study was carried out to investigate that the effect of grape seed oil (GSO) on Hypercholesterolemia in rats. Thirty-five rats were divided into 5 groups, group 1 was given the basal diet as a negative control group (-ve), group 2 was given the basal diet with GSO instead of soybean oil as a negative control for GSO. Groups (3, 4 and 5) were fed on high cholesterol diet (HCD) (1% cholesterol powder and 0.5% bile salt for 8 weeks), Group 3 was +ve control, group 4 and 5 were treatment groups which received GSO 2% and 4% daily with HCD. The results of this study indicated that the GSO caused an improvement in the blood lipids especially 4% GSO. The 4% GSO decreased serum TC, TG, LDL-c and VLDL significantly (P ˂ 0.05), and caused a significant increase in HDL-C level. Also, 2% GSO decreased TG, LDL-C and VLDL significantly (P ˂ 0.05), whereas the level of HDL-C showed significant increases. Subsequently, GSO enhanced the lipid ratios: atherogenic coefficient (AC), cardiac risk ratio (CRR), LDL-c to HDL-c ratio and atherogenic index of plasma (AIP). Moreover, the serum, liver function (AST, ALT and ALP) levels also, enhanced in the GSO groups. However, 4% GSO led to significant decrease in serum MDA and elevated serum GST. Otherwise, histopathological examination showed enhanced in the heart and aorta of rats compared with the +ve control group. This study indicates that GSO effective in lowering total cholesterol, triglyceride and LDL-c and increasing HDL-c. Therefore, GSO have hypocholesterolemic effect and might be effective to protect against the risk of CVD.
Grape Seed Oil, GSO, Lipid Profile, Cholesterol, Triglyceride, HDL
To cite this article
Reda Ahmed Mokhtar, Nefisa Hassan El-Banna, Mohammed Hamdy Haggag, Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats, International Journal of Nutrition and Food Sciences. Vol. 5, No. 5, 2016, pp. 310-317. doi: 10.11648/j.ijnfs.20160505.11
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