International Journal of Nutrition and Food Sciences
Volume 5, Issue 5, September 2016, Pages: 310-317
Received: Jul. 3, 2016;
Accepted: Jul. 13, 2016;
Published: Aug. 4, 2016
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Reda Ahmed Mokhtar, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Nefisa Hassan El-Banna, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
Mohammed Hamdy Haggag, Nutrition and Food Science Department, Faculty of Home Economics, Helwan University, Cairo, Egypt
This study was carried out to investigate that the effect of grape seed oil (GSO) on Hypercholesterolemia in rats. Thirty-five rats were divided into 5 groups, group 1 was given the basal diet as a negative control group (-ve), group 2 was given the basal diet with GSO instead of soybean oil as a negative control for GSO. Groups (3, 4 and 5) were fed on high cholesterol diet (HCD) (1% cholesterol powder and 0.5% bile salt for 8 weeks), Group 3 was +ve control, group 4 and 5 were treatment groups which received GSO 2% and 4% daily with HCD. The results of this study indicated that the GSO caused an improvement in the blood lipids especially 4% GSO. The 4% GSO decreased serum TC, TG, LDL-c and VLDL significantly (P ˂ 0.05), and caused a significant increase in HDL-C level. Also, 2% GSO decreased TG, LDL-C and VLDL significantly (P ˂ 0.05), whereas the level of HDL-C showed significant increases. Subsequently, GSO enhanced the lipid ratios: atherogenic coefficient (AC), cardiac risk ratio (CRR), LDL-c to HDL-c ratio and atherogenic index of plasma (AIP). Moreover, the serum, liver function (AST, ALT and ALP) levels also, enhanced in the GSO groups. However, 4% GSO led to significant decrease in serum MDA and elevated serum GST. Otherwise, histopathological examination showed enhanced in the heart and aorta of rats compared with the +ve control group. This study indicates that GSO effective in lowering total cholesterol, triglyceride and LDL-c and increasing HDL-c. Therefore, GSO have hypocholesterolemic effect and might be effective to protect against the risk of CVD.
Reda Ahmed Mokhtar,
Nefisa Hassan El-Banna,
Mohammed Hamdy Haggag,
Protective Effect of Grape Seed Oil on Hypercholesterolemia in Rats, International Journal of Nutrition and Food Sciences.
Vol. 5, No. 5,
2016, pp. 310-317.
Abdulrahman N., Abbas L., Darweesh H., Aref A., Ahmed M., Babaker M. et al., (2013): Effect of grape seed on some blood parameters and serum components of common CARP J Food Industries and Nutr. Sci. 3: 169-174.
Matthaus B. (2008): Virgin grape seed oil: Is it really a nutritional highlight? Eur. J. Lipid Sci. Technol. 110: 645–650.
Fernandes L., Casal S., Cruz R., Pereira J. and Ramalhosa E. (2013): Seed oils of ten traditional Portuguese grape varieties with interesting chemical and antioxidant properties. Food Research International. 50: 161-166.
Bail S., Stuebiger G., Krist S., Unterweger H. and Buchbauer G. (2008): Characterisation of various grape seed oils by volatile compounds, triacylglycerol composition, total phenols and antioxidant capacity. Food Chemistry. 108: 1122–1132.
Bravi M., Spinoglio F., Verdone N., Adami M., Aliboni A., D’Andrea A. et al., (2007): Improving the extraction of α-tocopherol-enriched oil from grape seeds by supercritical CO2. Optimisation of the extraction conditions. J Food Engineering. 78: 488–493.
Kim D., Jeon G., Sung J., Oh S., Hong H. and Lee J. (2010): Effect of grape seed oil supplementation on plasma lipid profiles in rats. Food Sci Biotechnol. 19 (1): 249-252.
Ranjbar-Zahedani M., Alinejad N., Zadeh S. and Mazloom Z. (2015): Comparison of the effects of edible oils: rice bran, grape seed, and canola on serum lipid profile and paraoxonase activity in hyperlipidemic rats. Int Cardiovasc Res J. 9 (1): 28-33.
Pilehvar A., Tabrizi B. and Javadi A. (2013): The effect of grape seeds oil on lipid content of serum in rats. Adv. Biores. 4: 21-25.
Wang Z., Zhang Z., Du N., Wang K. and Li L. (2015): Hepatoprotective Effects of grape seed procyanidin B2 in rats with carbon tetrachloride-induced hepatic fibrosis. Altern Ther Health Med. 2: 12-21.
Pardo J., Fernandez E., Rubio M., Alvarruiz A. and Alonso G. (2009): Characterization of grape seed oil from different grape varieties (Vitis vinifera). Eur J Lipid Sci Technol. 111: 188–193.
Irandoost P., Ebrahimi M., and Pirouzpanah S. (2013): Does grape seed oil improve inflammation and insulin resistance in overweight or obese women? Int J Food Sci Nutr. 64 (6): 706–710.
Lai X., Kang X., Zeng L., Li J., Yang Y. and Liu D. (2014): The protective effects and genetic pathways of thorn grape seeds oil against high glucoseinduced apoptosis in pancreatic β-cells. Complementary and Alternative Medicine. 14: 10.
Javadi S., Eftekhari A. and Farshid A. (2014): The effects of grape seed oil on histopathological changes of the pancreas, liver and plasma lipids in streptozotocin induced diabetic rats. The J Urmia University of Medical Sciences. 25 (7): 606–615.
Gómez A. M., Pereyra L. C. and Martínez E. (1996): Chem. Eng. J. 61: 227.
Hatzidimitriou E., Nenadis N. and Tsimidou M. Z. (2007): Changes in the catechin and epicatechin content of grape seeds on storage under different water activity (aw) conditions. Food Chemistry 105: 1504-1511.
ISO 5509, (2000): Animal and vegetable fats and oils – Preparation of methyl esters of fatty acids. International Standard Organization, Geneva (Switzerland).
ISO 5508, (1990): Animal and vegetable fats and oils – Analysis by gas chromatography of methyl esters of fatty acids. International Standard Organization, Geneva (Switzerland).
Reeves P. G., Nielsen F. H. and Fahmy G. G. (1993): AIN-93. Purified diets for laboratory rodents: Final report of the American Institute of Nutrition adhoc wriling committee on the reformulation of the AIN-76 A Rodent diet. J. Nutrition. 123: 1939-151.
Takako Y., Eun Ju C., Sumiyo S., Akiko S., Takuya O. and Yasuo S. (2006): The Protective Role of Chinese Prescription Kangen-karyu Extract on Diet-Induced Hypercholesterolemia in Rats. Biol. Pharm. Bull. 29 (4): 760-765.
Naito H. K. (1984a): Cholesterol. Kaplan A et al., Clin Chem The C. V. Mosby Co. St Louis. Toronto. Princeton; 1194-11206 and 437.
Fossati P. and Prencipe L. (1982): Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem 28 (10): 2077-2080.
Grove T. H. (1979): Effect of reagent pH on Determination of HDL Cholesterol by precipitation with Sodium Phosphotungstate-magnesium Clin Chem 25: 560-561.
Naito H. K. (1984b): High-density lipoprotein (HDL) cholesterol. Kaplan A et al. Clin Chem. The C. V. Mosby Co. St Louis. Toronto. Princeton 1207-1213 and 437.
Friedewald W. T., Levy R. I. and Fredrickson D. S. (1972): Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem. 18: 499-502.
IFCC, (1976): Expert Panel of Enzymes of the International Federation of Clinical Chemistry, Clin. Chem. Acta. 70: 19-42.
IFCC, (1980): Expert Panel of Enzymes of the International Federation of Clinical Chemistry, Clin. Chem. Acta. 105: 147-172.
GSCC, (1972): German Society of Clinical Chemistry Standard method for determination Alkaline Phosphatase (AP) activity. J. Clin. Chem. Clin. Biochem. 10: 290.
Satoh K. (1978): Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin, Chem. Acta. 90: 37-43.
Ohkawa H., Hishi W. and Yagi K. (1979): Assay for lipid peroxide in animal tissues by Thiobarbituric acid reaction. Anal. Biochem, 95: 351-358.
Habig W. and Pabst M. and Jakoby W. (1974): Glutathione Stransferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249: 7130–7139.
Mannervik B. and Danielson U. H. (1988): Glutathione transferases - structure and catalytic activity. CRC Crit. Rev. Biochem. 23: 283-337.
Bhardwaj S. Bhattacharjee J., Bhatnagar M. K. and Tyagi S. (2013): Atherogenic Index of Plasma, CASTELLI Risk Index and Atherogenic Coefficient- New Parameters in Assessing Cardiovascular Risk. IJPBS. 3 (3): 359-364.
Banchroft J. D., Stevens A. and Turner D. R. (1996): Theory and practice of histological techniques, Fourth Ed. Churchil Livingstone, New York, London Can J. Biochem Physiol. 37: 679-686.
SAS, (1996): Statistical Analysis System, User Guide Statistics. SAS Institute Inc. Editors, Cary, NC.
Weiss L. A., Pan L., Abney M. and Ober C. (2006): The sex-specific genetic architecture of quantitative traits in humans. Nat. Genet. 38: 218-222.
Rader D., Cohen J. and Hobbs H. (2003): Monogenic hypercholesterolemia: new insights in pathogenesis and treatment. J Clin Invest. 111: 1795–1803.
Brænne I., Reiz B., Medack A., Kleinecke M., Fischer M., Tuna S., et al., (2014): Whole-exome sequencing in an extended family with myocardial infarction unmasks familial hypercholesterolemia. BMC Cardiovascular Disorders. 14: 108.
Goldstein J. L. and Brown M. S. (2009): The LDL receptor. Arterioscler Thromb Vasc Biol. 29: 431-438.
Chung H., Choe C., Lee J., Park W. and Kang M. (2003): The effect of isoflavone and/or grape seed oil supplementation on blood lipid profiles and bone strength in ovariectomized female rats. Korean J Nutr. 36 (7): 667-674.
Nash D. T. (2004): Cardiovascular risk beyond LDL-C levels: Other lipids are performers in cholesterol story. Postgraduate Medicine. 116: 11–15.
Blu K., Zurabashvili D. Z., Turabelidze D. G., Zurabashvili Z. A. and Giorgobiani I. B. (2012): Fatty acids of grape seed oil and its biological activity as 1.0% and 2.5% food-additive. Georgian Med News. 207: 47-50.
Maheswari M. U. and Rao P. G. (2005): Antihepatotoxic effect of grape seed oil in rat. Indian J Pharmacol. 37 (3): 179-182.
Khudair K. and Aldabaj A. (2015): Effect of grape seed oil on hepatic function in adult male rabbits treated with sodium fluoride (Part-II). Advances in Animal and Veterinary Sciences. 3: 550-558.
Ismail A., Moawed F. and Mohamed M. (2015): Protective mechanism of grape seed oil on carbon tetrachloride-induced brain damage in γ-irradiated rats. J Photochemistry & Photobiology, B: Biology. 153: 317–323.
Kwok C., Wong C., Yau M. Y., Yu P. H., Shan Au A. L., Poon C. C. et al., (2010): Consumption of dried fruit of Crataegus pinnatifida (hawthorn) suppresses high-cholesterol diet-induced hypercholesterolemia in rats. J of Functional Foods. 2 (3): 179–186.