Immune Nutrient Content of Sea Urchin (Diadema setosum) Gonads
International Journal of Nutrition and Food Sciences
Volume 5, Issue 5, September 2016, Pages: 330-336
Received: Jul. 14, 2016; Accepted: Jul. 26, 2016; Published: Aug. 29, 2016
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Wa Ode Salma, Department of Nutrition, Faculty of Medicine, Halu Oleo University, Kendari, Indonesia
Sitti Wahyuni, Hasanuddin University Medical Research Centre, Makassar, Indonesia
Ilyas Yusuf, Pharmachology Laboratory, Pharmacy Academy of Bina Husada Foundation, Kendari, Indonesia
La Ode Muhammad Yasir Haya, Marine Science Department, Fisheries and Marine Science Faculty, Halu Oleo University, Kendari, Indonesia
Irawan Yusuf, Physiology Department, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Suryani As´ad, Department of Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
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Over population of marine sea urchins (Diadema setosum) may disrupt the growth of Crustose coralline causing the reduction of coral reefs growth. Consumption of Diadema setosum gonads as the alternative food may assists to preserve the balance of coral reefs ecosystem. The Objective of this study was to measure and evaluate the nutrient content of the gonad of Diadema setosum. Mass spectrophotometer was used to measure vitamin and albumin contents, Kjeldahl methods for protein content, and Atomization method for trace elements (Fe, Mg, and Zn) content. The presence of active compounds such as steroids, amino acids and antioxidants were identified by thin-layer chromatograph (TLC). Protein, albumin, vitamin A, vitamin E and trace elements (Fe, Mg, and Zn) were found in the gonad of Diadema setosum. Vitamin E (23.47 mg) was the highest nutrient content compared to other nutrient elements. The extracts of the gonad of Diadema setosum were found to have steroid, amino acids and antioxidant compounds. Overall, nutrient contents and active compounds in the gonad of Diadema setosum are essential components needed for immune system, therefore besides its potency as alternative food source, gonad of Diadema setosum has potency to become the source of immune-nutrient.
Diadema setosum, Gonad, Immune, Nutrient
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Wa Ode Salma, Sitti Wahyuni, Ilyas Yusuf, La Ode Muhammad Yasir Haya, Irawan Yusuf, Suryani As´ad, Immune Nutrient Content of Sea Urchin (Diadema setosum) Gonads, International Journal of Nutrition and Food Sciences. Vol. 5, No. 5, 2016, pp. 330-336. doi: 10.11648/j.ijnfs.20160505.13
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Clifton, J., Unsworth, R. K. F., Smith, D. J. (2010). Marine Research and Conservation in the Coral Triangle: The Wakatobi National Park. Environmental Science, Engineering and Techology series.: Nova Science Publishers. ISBN: 978-1-61668-473-0.
Wildlife Conservation Society (2011). Sea urchins destroy reef building algae in overfished sites on Kenya's coast. Science Daily. Available from: Accesed on December 1, 2015.
Baumiller, T. K., Mooi, R., Charles, G. M. (2008). Urchins in the meadow: paleobiological and evolutionary implications of cidaroid predation on crinoids. Paleobiology. 34 (01): p. 22-34.
O'Leary, J. K., Timothy, R. M. (2010). Trophic cascades result in large-scale coralline algae loss through differential grazer effects. Ecology. 91 (12): p. 3584-3597.
Bragadeeswaran, Kumaran, S., Prasath Sankar, P., Prabahar, R. (2013). Bioactive potential of sea urchin Temnopleurus toreumaticus from Devanampattinam, Southeast coast of India. Journal of Pharmacy and Alternative Medicine. 2 (3): p. 9-18.
Dincer, T., Cakli, S. (2007). Chemical composition and biometrical measurements of the Turkish sea urchin (Paracentrotus lividus, Lamarck, 1816). Critical reviews in food science and nutrition. 47 (1): p. 21-26.
Chen, G., Weng, Z. X., Chi, C. L., Juang, P., Jian, W. Q., Feng, C., Yue, J. (2010). A comparative analysis of lipid and carotenoid composition of the gonads of Anthocidaris crassispina, Diadema setosum and Salmacis sphaeroides. Food chemistry. 120 (4): p. 973-977.
National Standardization Agency of Indonesia. Food and beverages. Test methods. SNI 01-2891-1992; Available from: Accesed on September 1, 2014.
Jork, H., Werner, F., Walter, F., Hans, W. (1990). Thin-layer chromatography. Reagents and detection methods. Physical and chemical detection methods: fundamentals, reagents I. Volume 1a: VCH, Weinheim. (ISBN 3-527-27834-6). xv+ 464 pp. Price DM 148.00. Analytica Chimica Acta. 237: p. 511-512.
Celescerl, L. S., Greenberg, A. E., Eaton, A., D. (1999). Standard methods for the examination of water and wastewater 20th edition. American Public Health Association, American Water Works Association, Water Environment Federation: (Section 3111B). ISBN 0-87553-235-7.
Peng, J., Jian-Ping, Y., Jiang-Hai, W. (2012). Effect of diets supplemented with different sources of astaxanthin on the gonad of the sea urchin Anthocidaris crassispina. Nutrients. 4 (8): p. 922-934.
Spangenberg, B. (2008). Derivatization, Detection, (Quantification) and Identification of Compounds Online, in Thin layer chromatography in phytochemistry, Waksmundzka-Hajnos M, Sherma J, Kowalska T. Editors, CRC Press: New York: p. 175-189.
Gredel, S. (2011). Nutrition and Immunity in Man.: ILSI Europe concise monograph seriers, 2nd edition. ISBN: 9789078637271.
Wintergerst, E. S., Maggini, S., Hornig, D. H. (2007). Contribution of selected vitamins and trace elements to immune function. Annals of Nutrition and Metabolism. 51 (4): p. 301-323.
Mocchegiani, E., Laura, C., Robertina, G., Francesco, P., Andrea, B., Marco M. (2012). Micronutrient (Zn, Cu, Fe)–gene interactions in ageing and inflammatory age-related diseases: Implications for treatments. Ageing research reviews. 11 (2): p. 297-319.
Vasanthi, H. R., Parameswari, R., Das, D. K. (2012). Multifaceted role of tocotrienols in cardioprotection supports their structure: function relation. Genes & nutrition. 7 (1): p. 19-28.
Mann, J., Truswell, S. (2012). Essentials of human nutrition: Oxford University Press.
Mocchegiani, E., Laura, C., Robertina, G., Marco, M., Andrea, B., Francesco, P., et al. (2014) Vitamin E–gene interactions in aging and inflammatory age-related diseases: Implications for treatment. A systematic review. Ageing research reviews. 14: p. 81-101.
Hall, J. A., Grainger, J. R., Spencer, S. P., Belkaid, Y. (2011). The role of retinoic acid in tolerance and immunity. Immunity. 35 (1): p. 13-22.
Fragoso, Y. D., Stoney, P. N., McCaffery, P. J, (2014). The evidence for a beneficial role of vitamin A in multiple sclerosis. CNS drugs 28 (4): p. 291-299.
21 Mora, J. R., Iwata, M., Von Andriano, U. H. (2008). Vitamin effects on the immune system: vitamins A and D take centre stage. Nature Reviews Immunology. 8 (9): p. 685-698.
WHO, Guideline. (2011). vitamin A supplementation in infants and children 6-59 months of age: Geneva: World Health Organization.
Imdad, A., Yakoob, M. Y., Sudfeld, C., Haider, B. A., Black, R. E., Bhutta, Z. A. (2011). Impact of vitamin A supplementation on infant and childhood mortality. BMC Public Health. 11 (Suppl 3): p. S 20.
United States Department of Agriculture, Agricultural Research Services, National Nutrient data base for standard reference, release 28. Basic Report: 15049, Fish, mackerel, king, raw.; Available from: Accesed on March 14, 2016.
Sitar, M. E., Aydin, S., Cakatay, U. (2013). Human serum albumin and its relation with oxidative stress. Clin Lab. 59 (9-10): p. 945-952.
Bruschi, M., Candiano, G., Santucci, L., Ghiggeri, G. M. (2013). Oxidized albumin. The long way of a protein of uncertain function. Biochimica et Biophysica Acta (BBA)-General Subjects. 1830 (12): p. 5473-5479.
Gupta, D., Lis, C. G. (2010). Pretreatment serum albumin as a predictor of cancer survival: a systematic review of the epidemiological literature. Nutrition journal. 9 (1): p. 1.
Mustafa, A., Widodo, M. A., Yohanes, K. (2012). Albumin and zinc content of snakehead fish (Channa striata) extract and its role in health. International Journal of Science and Technology. 1 (2): p. 1-8.
Rodriguez, M. M., Simental Mendia, L. E., Zambrano, G. G., Guerrero, R. F. (2012). The role of magnesium in type 2 diabetes: a brief based-clinical review. Magnesium Research. 24 (4): p. 156-162.
Sojka, J. (1995). Magnesium supplementation and osteoporosis. Nutrition reviews. 53 (3): p. 71-74.
Nica, A. S., Adela, C., Mirela, V., Anca Mirela, I., Denis, P., Virgil, M. (2015). Magnesium supplementation in top athletes-effects and recommendations. Medicina Sportiva: Journal of Romanian Sports Medicine Society. 11 (1): p. 2482.
Kass, L., Weekes, J., Capenter, L. (2012). Effect of magnesium supplementation on blood pressure: a meta-analysis. European journal of clinical nutrition. 66 (4): p. 411-418.
Doyle, L. W., Crowther, C. A., Middleton, P., Marret, S., Rouse, D. (2009). Magnesium sulphate for women at risk of preterm birth for neuroprotection of the fetus. Cochrane Database Syst Rev. 1 (1).
Volpe, S. L. (2013). Magnesium in disease prevention and overall health. Advances in Nutrition: An International Review Journal. 4 (3): p. 378 S-383 S.
Larrson, S. C., Orsinin, N., Wolk, A. (2012). Dietary magnesium intake and risk of stroke: a meta-analysis of prospective studies. The American journal of clinical nutrition. 95 (2): p. 362-366.
Guerrero-Romero, F., Rodríguez-Morán, M. (2013). [Oral magnesium supplementation: an adjuvant alternative to facing the worldwide challenge of type 2 diabetes?]. Cirugia y cirujanos. 82 (3): p. 282-289.
United States Department of Agriculture, Agricultural Research Services, National Nutrient data base for standard reference, release 28. Basic Report: 01128, Egg, whole, cooked, fried. Available from: Accesed on March 14, 2016.
United States Department of Agriculture, Agricultural Research Services, National Nutrient data base for standard reference, release 28. Basic Report: 35166, Fish, salmon, red, (sockeye), canned, smoked (Alaska Native). Available from: Accesed on March 14, 2016.
Abramowski, W. S., Waeber, G., Gassner, C., Buser, A., Frey, B. M, et al. (2014) Physiology of iron metabolism. Transfusion Medicine and Hemotherapy. 41 (3): p. 213-221.
Gibson, R. S. (2005). Principles of nutritional assessment: Oxford university press, USA.
Black RE., Victora, C. G., Walker, S. P., Bhutta, Z. A., Christian, P., de Onis, M. (2013). Maternal and child undernutrition and overweight in low-income and middle-income countries. The Lancet. 382 (9890): p. 427-451.
Rahman, M. M., Abe, K. S., Rahman, S. M., Kanda, M., Narita, S., Bilano, V. (2016). Maternal anemia and risk of adverse birth and health outcomes in low-and middle-income countries: systematic review and meta-analysis. The American journal of clinical nutrition. p. ajcn 107896.
McCall, K. A., Huang, C., Fierke, C. A. (2000). Function and mechanism of zinc metalloenzymes. The Journal of nutrition. 130 (5): p. 1437 S-1446 S.
Prasad, A. S. (2012). Discovery of human zinc deficiency: 50 years later. Journal of Trace Elements in Medicine and Biology. 26 (2): p. 66-69.
Imdad, A., Bhutta, Z. A. (2011). Effect of preventive zinc supplementation on linear growth in children under 5 years of age in developing countries: a meta-analysis of studies for input to the lives saved tool. BMC public health. 11 (Suppl 3): p. S 22.
Zhong, W., McClain, C. J., Cave, M., Kang, Y. J., Zhou, Z. (2010). The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. American Journal of Physiology-Gastrointestinal and Liver Physiology. 298 (5): p. G 625-G 633.
Aggarwal, R., Sentz, J., Miller, M. A. (2007). Role of zinc administration in prevention of childhood diarrhea and respiratory illnesses: a meta-analysis. Pediatrics. 119 (6): p. 1120-1130.
Lassi, Z. S., Haider, B. A., Butta, Z. A. (2010). Zinc supplementation for the prevention of pneumonia in children aged 2 months to 59 months. Cochrane Database Syst Rev. 12.
As' ad, S., Irawan, Y. (2013) The effects of zinc supplementation on the TNF-[alpha] profile and diarrhea in severely malnourished children of low income family. Medical Journal of Indonesia. 12 (4): p. 247.
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