Antibacterial Effects of Guava (Psidium guajava L.) Extracts Against Food Borne Pathogens
International Journal of Nutrition and Food Sciences
Volume 6, Issue 1, January 2017, Pages: 1-5
Received: Oct. 19, 2016; Accepted: Nov. 19, 2016; Published: Dec. 20, 2016
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Authors
Jasmin Ara Farhana, Department of Human Nutrition and Dietetics, Patuakhali Science and Technology University, Patuakhali, Bangladesh
Md. Faruk Hossain, Institute of Nutrition and Food Science, University of Dhaka, Dhaka, Bangladesh
Aleya Mowlah, Institute of Nutrition and Food Science, University of Dhaka, Dhaka, Bangladesh
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Abstract
Guava is commonly used as a medicine against gastroenteritis and child diarrhea by those who cannot afford or do not have access to antibiotics. The present study was conducted to clarify the possible effects of antimicrobial activities of guava extracts. Its antimicrobial activities was determined against five food-borne pathogens: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Bacillus cereus (BTCC 19), Shigella sonnei (BTCC) and Salmonella typhi (BTCC 197) using disc diffusion method at four different concentrations: 10%, 50%, 75% and 100%. Its antibacterial activity was also determined at three different temperatures: 50°C, 75°C and 100°C. The test organisms differed in their reaction to these different extracts, but as a whole inhibition of bacterial growth increased with the increased concentration. All the samples showed antibacterial activity after heat treatment at 50°C, 75°C and 100°C suggesting that the temperature does not affect the activity. Guava extracts showed higher antibacterial activity against gram positive bacteria compared to gram negative bacteria. None of the extracts (10%) showed antibacterial activity against these pathogens. The results of the present study assume that guava extracts possess compounds containing antibacterial properties that can potentially be useful to control food borne pathogens especially diarrhea causing organisms.
Keywords
Antibacterial Effect, Food, Food Borne Pathogen, Test Organism, Media
To cite this article
Jasmin Ara Farhana, Md. Faruk Hossain, Aleya Mowlah, Antibacterial Effects of Guava (Psidium guajava L.) Extracts Against Food Borne Pathogens, International Journal of Nutrition and Food Sciences. Vol. 6, No. 1, 2017, pp. 1-5. doi: 10.11648/j.ijnfs.20170601.11
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Lutterodt, G. D. Inhibition of gastrointestinal release of acetylcholine by quercetin as a possible mode of action of Psidium guajava leaf extracts in the treatment of acute diarrhoeal diseases. J Ethnopharmacology. 25:235-247, 1989.
[2]
Alnieida, C. C.; Karnikowski, M. G.; Flieto, R. and Baldis-serotto, B. - Analysis of antidiarrhoic effect of plantsused in popular medicine. Rev. Saude Publica. 29: 428-433, 1995.
[3]
Hidetoshi, A. and Danrio, G. - Isolation of antimi-crobial compounds from guava (Psidium guajava L.) and their structural elucidation. Biosci. Biotechnol. Biochem. 66: 1727-1730, 2002.
[4]
Abdeirahirn, S. I.; Almadboul, A. Z.; Omer, M. E. A. and Elegami, A. - Antimicrobial activity of Psidium guajava L. Fitoterapia. 73:713-715, 2002.
[5]
Jaiarj, P.; Khoohaswan, P; Wongkrajng, Y; Peungricha, P.; Suriyawong, P; Sumalsaraya, M. L. and Ruangsom-boon, M. L. - Anticough and antimicrobial activities of Psidiuni guajava Linn. leaf extract. J. Ethnopharmacol. 67: 203-212, 1999.
[6]
Gutiérrez, R. M. P; Mitchell, S and Solis, R. V. - Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 117: 1-27, 2008.
[7]
Cown, N. M. - Plant product as antimicrobial agents. Cln. Microbiol Rev. 12: 564, 1999.
[8]
Nostro, A; Germano, M. P. D; Angelo, V; Marino, A and Cannatell, M. A. - Extraction method and bioautography for evaluation of medical plant antimicrobial activity. Lett. Appl. Microbiol. 30: 379, 2000.
[9]
Almagboul, A. Z.; Bashir, A. K.; Farouk, A.; Salih A. K. M. Antimicrobial activity of certain Sudanese plants used in folkloric medicine. Screening for antibacterial activity. Fitoterapia 56, 331-337, 1985.
[10]
Artizzu, N.; Bonsignore, L.; Cottiglia, F.; Loy, G. Studies of the diuretic and antimicrobial activity of Cynodon dactylon essencial oil. Fitoterapia 66, 174-175, 1995.
[11]
Ikram, M.; Inamul, H. Screening of medicinal plants for antimicrobial activities. Fitoterapia 55, 62-64, 1984.
[12]
Izzo, A. A.; Di Carlo, G.; Biscardi, D.; Fusco, R.; Mascolo, N.; Borreli, F.; Capasso, F.; Fasulo, M. P.; Autore, G. Biological screening of Italian medicinal plants for antibacterial activity. Phytother. Res. 9, 281-286, 1995.
[13]
Kubo, L.; Muroi, H.; Himejima, M. Structure-antibacterial activity relationships of anacardic acids. J. Agri. Food Chem. 41, 1016-1019, 1993.
[14]
Shapoval, E. E. S.; Silveira, S. M.; Miranda, M. L.; Alice, C. B.; Henriques, A. T. Evaluation of some pharmacological activities of Eugenia uniflora. J. Ethnopharmacol. 44, 136-142, 1994.
[15]
Sousa, M.; Pinheiro, C.; Matos, M. E. O.; Matos, F. J.; Lacerda, M. I.; Craveiro, A. A. Constituintes Químicos de Plantas Medicinais Brasileiras. Universidade Federal do Ceará, Fortaleza, p. 385-388, 1991.
[16]
Jansen, A. M.; Cheffer, J. J. C.; Svendsen, A. B. Antimicrobial activity of essencial oils: a 1976-1986 literature review. Aspects of test methods. Planta Med. 40: 395-398, 1987.
[17]
Saxena, G.; McCutcheon, A. R.; Farmer, S.; Towers, G. H. N.; Hancock, R. E. W. Antimicrobial constituents of Rhus glabra. J. Ethnopharmacol. 42: 95-99, 1994.
[18]
http://www.naturalbird.com/mcwatters/medicinal.htm
[19]
Fu, X.; Deng, S.; Su, G.; Zeng, Q. and Shi, S. - Isolating high-quality RNA from mangroves without liquid nitrogen. Plant Molecular Biology Reporter. 22 (2): 197a-198e, 2004.
[20]
Gonçalves, F. A.; Andrade Neto, M.; Bezerra, J. N. S.; Macrae, A.; Sousa, O. V.; Fonteles-Filho, A. A. & Vieira, R. H. S. F. - Antibacterial activity of guava, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp. Xiphopenaeus kroyeri (Heller). Rev. Inst. Med. trop. S. Paulo, 50 (1): 11-15, 2008.
[21]
Malaviya, A and Mishra, N. - Antimicrobial activity of tropical fruits. Biological Forum – An International Journal, 3(1): 1-4: 2011
[22]
Hoque, M. M.; Bari, M. L.; Inatsu, Y; Vijay, K; Juneja and Kawamoto, S. - Antibacterial Activity of Guava (Psidium guajava L.) and Neem (Azadirachta idica A. Juss.) extracts Against Foodborne Pathogens and Spoilage Bacteria. National Food Research Institute, Japan. 4 (4):481-488, 2007.
[23]
Ismail, M; Minhas, P. S.; Khanum, F; Sahana, V. M. and Sowmya, C. - Antibacterial Activity of Leaves Extract of Guava (Psidium Guajava). International Journal of Research in Pharmaceutical and Biomedical Sciences. 3 (1): 1, 2012.
[24]
Bauer, L; Kohlich, A; Hirschwehr, R; Siemann, U; Ebner, H; Scheiner, O; Kraft, D; Ebner, C. Food allergy to honey: pollen or bee products? Characterization of allergenic proteins in honey by means of immunoblotting. J Allergy Clin Immunol; 97 (1 Pt 1): 65-73, 1996.
[25]
Shelef, L. A. – Antimicrobial effects of spices. J Food Saf. 6: 29-44, 1983.
[26]
Farag, R. S.; DAW, Z. Y.; Hewedi, F. M. and El-Baroty, G. S. A. - Antimicrobial activity of some Egyptian spice essential oils. J. Food Prot. 52: 665-667, 1989.
[27]
Gnan, S. O. and Demello, M. T. - Inhibition of Staphylococcus aureus by aqueous Psidium guajava leaves extracts. J Ethnopharmacol. 68:103-108, 1999.
[28]
Tona, L. - Biological screening of traditional preparations from some medicinal plants used as antidiarrhoeal in Kinshasa, Congo. Phytomedicine. 6: 59–66, 1999.
[29]
Oliver-Bever, B. - Medicinal Plants in Tropical WestAfrica. Cambridge University Press, Cambridge, United Kingdom, 1986.
[30]
Sanches, N.R.; Garcia, D. A.; Schiavini, M. S.; Naka-mura, C. V. and Filho, B. P. D. - An evaluation of anti-bacterial activities of Psidiuni gitajava. Brazilian Arch.Biol. Technol. 48:429-436, 2005.
[31]
Ekwenye, U. N; Elegalam, N. N. - Antibacterial activity of Ginger (Zingiber officinale Roscoe) and garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. Int J Mole Med Adv Sci 1 (4): 411-416, 2005.
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