Prevalence of Highly Multi-Drug Resistant Salmonella Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia
International Journal of Nutrition and Food Sciences
Volume 6, Issue 1, January 2017, Pages: 39-44
Received: Dec. 6, 2016; Accepted: Dec. 26, 2016; Published: Jan. 23, 2017
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Authors
Abou Kebbeh, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Bryan Anderson, College of Public Health, The University of Iowa, Iowa, United States of America
Haruna S. Jallow, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Olliemattou Sagnia, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Joseph Mendy, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Yaya Camara, Epidemiology and Disease Control Department, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Saffiatou Darboe, Department of Microbiology, Medical Research Council Unit, Fajara, The Gambia
Sana M. Sambou, Epidemiology and Disease Control Department, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Ignatious Baldeh, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
Bakary Sanneh, National Public Health Laboratories, Ministry of Health and Social Welfare, Kotu Layout, Kotu, The Gambia
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Abstract
Background: Salmonella spp are among the most common food borne pathogens. Food handlers play an important role in the production of food products, in which they can contribute to the transmission of salmonellosis. The probability of food contamination depends mainly on the health status and personal hygiene of the food handlers and their choice of managing their health could give rise to contamination of food by multi drug resistant bacteria. Multi Drug Resistance is of global concern and poses a public health threat in combating diseases. In the developing countries there is paucity on the prevalence of salmonella carriage among food handlers. This study aims to investigate the prevalence of salmonella and to determine their antimicrobial resistance pattern. Method: A total of 500 stool samples from different food handlers were collected and analyzed with completed questionnaires. For standard isolation and identification of salmonella isolates; stool samples were enriched in buffered peptone water, standard culture and biochemical tests were used. Antimicrobial susceptibility Test (AST) was carried out using Clinical Laboratory and Standard Institute (CLSI-2015) protocol disc diffusion method. The data were analyzed using SPSS version 16 and Microsoft excel version 2010 to determine the risk factors. Results: Of 500 participants with the mean age of 38.15, 497(99.4%) were all females. Most of the participants wash their hands under running water and 271(54.2%) were certified on food handling. Among the risk factors, consuming medicines/antibiotics from street vendors showed statistical significant of salmonella carriage with (P=0.011). It was found that 13(2.6%) were salmonella carriers. AST performed on the 13 isolates show that; 13(100%), 12(92.3%), 7(53.8%), 9(69.2%) and 9(69.2%) were resistant to Ampicillin, Erythromycin, and Tetracycline, ceftriaxone and cefotaxime respectively. And 13(100%), 12(92.3%) and 7(53.8) were sensitive to Imipenem, chloramphenicol and gentamycin respectively. Conclusion: This study had found multidrug resistant salmonella isolates carriers amongst food handlers who could serve as potential reservoirs for the transmission of these infections in the communities. Thus, it is crucial to implement regular screening of food handlers and health education on food safety.
Keywords
Antimicrobial Susceptibility Test, Food handlers, Salmonella, The Gambia
To cite this article
Abou Kebbeh, Bryan Anderson, Haruna S. Jallow, Olliemattou Sagnia, Joseph Mendy, Yaya Camara, Saffiatou Darboe, Sana M. Sambou, Ignatious Baldeh, Bakary Sanneh, Prevalence of Highly Multi-Drug Resistant Salmonella Fecal Carriage Among Food Handlers in Lower Basic Schools in The Gambia, International Journal of Nutrition and Food Sciences. Vol. 6, No. 1, 2017, pp. 39-44. doi: 10.11648/j.ijnfs.20170601.17
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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