Identification and Characterization of a Bacteriocin-Like Substance, Produced by Leuconostoc mesenteroides, as a Bio-Preservative Against Listeria monocytogenes
International Journal of Nutrition and Food Sciences
Volume 6, Issue 4, July 2017, Pages: 167-171
Received: Jun. 27, 2017;
Published: Jun. 27, 2017
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Hai Chi, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China
Xueying Li, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China
Zhen Kuang, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, China
Xianshi Yang, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China
By conducting a systemic screening from Chinese traditional Tofu, a bacteriocin producer with broad antimicrobial spectrum was found. The producer was identified by 16S rDNA sequencing, and it was identified as Leuconostoc mesenteroides. The producer possessed typical bacteriocin traits, such as be heat-stable and proteinase-labile. Its inhibition spectrum contained many distantly related genera of Firmicutes, comprising most lactic acid bacteria (LAB) as well as problematic species of Bacillus, Listeria and Staphylococcus. In addition, the bacteriocin producer showed significantly inhibition effects on controlling Listeria monocytogenes in milk. Taken together, the results indicate that this is a novel and potent bacteriocin from Leu.mesenteroides and that its very broad inhibition spectrum can be use in food preservation as well as in infection treatments caused by Gram-positive pathogens.
Identification and Characterization of a Bacteriocin-Like Substance, Produced by Leuconostoc mesenteroides, as a Bio-Preservative Against Listeria monocytogenes, International Journal of Nutrition and Food Sciences.
Vol. 6, No. 4,
2017, pp. 167-171.
Hill, B., Smythe, B., Lindsay, D. & Shepherd, J. (2012) Microbiology of raw milk in New Zealand. Int J Food Microbiol, vol.157, pp. 305-8.
Jayarao, B. M., Donaldson, S. C., Straley, B. A., Sawant, A. A., Hegde, N. V. & Brown, J. L. (2006) A survey of foodborne pathogens in bulk tank milk and raw milk consumption among farm families in pennsylvania. J Dairy Sci, vol.89, pp. 2451-8.
Oliver, S. P., Jayarao, B. M. & Almeida, R. A. (2005) Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodborne Pathog Dis, Vol.2, pp. 115-29.
Van Kessel, J. S., Karns, J. S., Gorski, L., McCluskey, B. J. & Perdue, M. L. (2004) Prevalence of Salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies. J Dairy Sci, vol.87, pp. 2822-30.
Osman, K. M., Zolnikov, T. R., Samir, A. & Orabi, A. (2014) Prevalence, pathogenic capability, virulence genes, biofilm formation, and antibiotic resistance of Listeria in goat and sheep milk confirms need of hygienic milking conditions. Pathog Glob Health, vol. 108, pp. 21-9.
Kamana, O., Ceuppens, S., Jacxsens, L., Kimonyo, A. & Uyttendaele, M. (2014) Microbiological quality and safety assessment of the rwandan milk and dairy chain. J Food Prot, vol.77, pp. 299-307.
Moshtaghi, H. & Mohamadpour, A. A. (2007) Incidence of Listeria spp. in raw milk in Shahrekord, Iran. Foodborne Pathog Dis, vol.4, pp. 107-10.
Normanno, G., Firinu, A., Virgilio, S., Mula, G., Dambrosio, A., Poggiu, A., Decastelli, L., Mioni, R., Scuota, S., Bolzoni, G., Di Giannatale, E., Salinetti, A. P., La Salandra, G., Bartoli, M., Zuccon, F., Pirino, T., Sias, S., Parisi, A., Quaglia, N. C. & Celano, G. V. (2005) Coagulase-positive Staphylococci and Staphylococcus aureus in food products marketed in Italy. Int J Food Microbiol, vol.98, pp. 73-9.
Huijps, K., Lam, T. J. & Hogeveen, H. (2008) Costs of mastitis: facts and perception. J Dairy Res, vol.75, pp. 113-20.
Viguier, C., Arora, S., Gilmartin, N., Welbeck, K. & O'Kennedy, R. (2009) Mastitis detection: current trends and future perspectives. Trends Biotechnol, vol.27, pp. 486-93.
Jack, R. W., Tagg, J. R. & Ray, B. (1995) Bacteriocins of gram-positive bacteria. Microbiol Rev, vol.59, pp. 171-200.
Hu, M., Zhao, H., Zhang, C., Yu, J. & Lu, Z. (2013) Purification and characterization of plantaricin 163, a novel bacteriocin produced by Lactobacillus plantarum 163 isolated from traditional Chinese fermented vegetables. J Agric Food Chem, vol.61, pp. 11676-82.
Zendo, T. (2013) Screening and characterization of novel bacteriocins from lactic acid bacteria. Biosci Biotechnol Biochem, vol.77, pp. 893-9.
Awaisheh, S. S. & Ibrahim, S. A. (2009) Screening of antibacterial activity of lactic acid bacteria against different pathogens found in vacuum-packaged meat products. Foodborne Pathog Dis, vol.6, pp. 1125-32.
Birri, D. J., Brede, D. A., Tessema, G. T. & Nes, I. F. (2013) Bacteriocin production, antibiotic susceptibility and prevalence of haemolytic and gelatinase activity in faecal lactic acid bacteria isolated from healthy Ethiopian infants. Microb Ecol, vol.65, pp. 504-16.
Abee, T., Krockel, L. & Hill, C. (1995) Bacteriocins: modes of action and potentials in food preservation and control of food poisoning. Int J Food Microbiol, vol.28, pp. 169-85.
Diep, D. B., Skaugen, M., Salehian, Z., Holo, H. & Nes, I. F. (2007) Common mechanisms of target cell recognition and immunity for class II bacteriocins. Proc Natl Acad Sci U S A, vol.104, pp. 2384-9.
Kanatani, K. & Oshimura, M. (1994) Plasmid-associated bacteriocin production by a Lactobacillus plantarum strain. Biosci Biotechnol Biochem, vol.58, pp. 2084-6.
Hitchener, B. J., Egan, A. F. & Rogers, P. J. (1982) Characteristics of lactic acid bacteria isolated from vacuum-packaged beef. J Appl Bacteriol, vol.52, pp. 31-7.
Papamanoli, E., Tzanetakis, N., Litopoulou-Tzanetaki, E. & Kotzekidou, P. (2003) Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage in respect of their technological and probiotic properties. Meat Science, vol.65, pp. 859-867.
Ryan, M. P., Rea, M. C., Hill, C. & Ross, R. P. (1996) An application in cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad-spectrum bacteriocin, lacticin 3147. Appl Environ Microbiol, vol.62, pp. 612-9.
Villani, F., Aponte, M., Blaiotta, G., Mauriello, G., Pepe, O. & Moschetti, G. (2001) Detection and characterization of a bacteriocin, garviecin L1-5, produced by Lactococcus garvieae isolated from raw cow's milk. J Appl Microbiol, vol.90, pp. 430-9.
Cleveland, J., Montville, T. J., Nes, I. F. & Chikindas, M. L. (2001) Bacteriocins: safe, natural antimicrobials for food preservation. Int J Food Microbiol, vol.71, pp.1-20.
Galvez, A., Abriouel, H., Lopez, R. L. & Ben Omar, N. (2007) Bacteriocin-based strategies for food biopreservation. Int J Food Microbiol, vol.120, pp. 51-70.
Cotter, P. D., Hill, C. & Ross, R. P. (2005) Bacteriocins: developing innate immunity for food. Nat Rev Microbiol, vol.10, pp. 777-88.
Dubeuf, J.-P. (2005) Structural, market and organisational conditions for developing goat dairy production systems. Small Ruminant Research, vol.60, pp. 67-74.
Duarte, A. F. d. S., Ceotto, H., Coelho, M. L. V., Brito, M. A. V. d. P. & Bastos, M. d. C. d. F. (2013) Identification of new staphylococcins with potential application as food biopreservatives. Food Control, vol.32, pp. 313-321.