International Journal of Nutrition and Food Sciences
Volume 7, Issue 4, July 2018, Pages: 116-120
Received: Apr. 23, 2018;
Accepted: May 19, 2018;
Published: Jun. 25, 2018
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Hafiz Shehzad Muzammil, National Institute of Food Science and Technology, University of Agricultural Faisalabad, Faisalabad, Pakistan
Barbara Rasco, School of Food Science, Washington State University, Pullman, Washington, USA
Muhammad Junaid, Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore, Pakistan
To evaluate the effect of inulin and glycerol supplementation on the viability of probiotic and yogurt bacterial cultures in frozen yogurt, this study was conducted. The frozen yogurt mixture was prepared with different types of probiotics (Lactobacillus acidophilus and Bifidobacterium lactis) along with commercial yogurt starter culture (Streptococcus thermophilus and Lactobacillus bulgaricus). Different concentrations of inulin (2%, 4%, and 6%) and glycerol (1%, 2%, 3%, and 4%) were also supplemented. The cultural survival rates before and after freezing and with 3 weeks regular intervals up to 12 weeks were examined. The viability loss during freezing process of S. thermophilus and L. bulgaricus were recorded 0.2-0.3 log cycles. While, in probiotic cultures this was observed 0.2-0.5 log cycles in L. acidophilus and 0.1-0.2 log cycles in B. lactis concentration. The glycerol 4% supplementation in this study has shown most significant improvement in viability (P<0.05).
Hafiz Shehzad Muzammil,
Viability of Probiotics in Frozen Yogurt Supplemented with Inulin and Glycerol, International Journal of Nutrition and Food Sciences.
Vol. 7, No. 4,
2018, pp. 116-120.
Cheikhyoussef A, Pogor N, Chen W., Zhang H. (2008), Antimicrobial proteina- ceous compounds obtained from bifidobacteria: from production to their application. Int. Journal Food Microbiol, 125, 215–222.
Cleusix V., Lacroix C., Vollenweider S., LeBlay G. (2008), Glycerol induces reuterin production and decreases Escherichia coli population in an in vitro model of colonic fermentation with immobilized human feces. Microbiol. Ecology, 63, 56–64.
Delzenne N. M., Neyrinck A. M., Backhed F., Cani P. D. (2011): Targeting gut microbiota in obesity: effects of prebiotics and probiotics. Nature Reviews Endocrinol. J., 7: 639–646.
Kumar M., Verma V., Nagpal R., Kumar A., Behare P. V., Singh B., Aggarwal P. K. (2011), Anticarcinogenic effect of probiotic fermented milk and chlorophyllin on aflatoxin-B1 induced liver carcinogenesis in rats. British J. Nutrition, 107, 1006–1016.
Shah N. P. (2007), Functional cultures and health benefits. Int. Dairy J., 17, 1262–1277.
Takeda K., Suzuki T., Shimada S. I., Shida K., Nanno M., Okumura K. (2006), Interleukin-12 is involved in the enhancement of human natural killer cell activity by Lactobacillu scasei Shirota. Clinic Exper. Immunol, 146, 109–115.
Boylston T. D., Vinderola C. G., Ghoddusi H. B., Reinheimer J. A. (2004), Incorporation of bifidobacteria into cheese: challenges and rewards. Int. Dairy J., 14, 375-387.
Jayamanne V. S., Adams M. R. (2006), Determination of survival, identity and stress resistance of probiotic bifidobacteria in bio‐yoghurts. Applied Microbiol., 42(3), 189-194.
Talwalkar A., Miller C. W., Kailasapathy K., Nguyen H. M. (2004), Effect of packaging materials and dissolved oxygen on the survival of probiotic bacteria in yoghurt. Int. J. Food Sci. Technol., 39, 605–611.
Cruz A. G., Antunes A. E. C., Sousa A. L. O., Faria J. A. F., Saad S. M. I. (2009), Ice-cream as a probiotic food carrier. Food Res. Int., 42 (9), 1233–1239.
Turgut T., Cakmakci S. (2009), Investigation of the possible use of probiotics in ice cream manufacture. Int. J. Dairy Technol., 62 (3), 444–451.
Trindade C. S. F., Bernardi S., Bodini R. B., Balieiro J. C. D. C., Almeida E. D. (2006), Sensory acceptability and stability of probiotic microorganisms and vitamin C in fermented acerola ice cream. J. Food Sci., 71, 492-495.
Magarinos H., Selaive S., Costa M., Flores M., Pizarro O. (2007), Viability of probiotic micro-organisms (Lactobacillus acidophilus La-5 and Bifidobacterium animalis subsp. Lactis Bb-12) in ice cream. Int. J. Dairy Technol., 60(2), 128-134.
Soukoulis C., Lebesi D., Tzia C. (2009), Enrichment of ice cream with dietary fiber: effects on rheological properties, ice crystallization and glass transition phenomena. Food Chem., 115, 665-671.
Buriti F. C. A., Castro I. A., Saad S. M. I. (2010), Viability of Lactobacillus acidophilus in synbiotic guava mousses and its survival under in vitro simulated gastro-intestinal conditions. Int. J. Food Microbiol, 137: 121-129.
Donkor O. N., Nilmini S. L. I., Stolic P., Vasiljevic T., Shah N. P. (2007), Survival and activity of selected probiotic organisms in set-type yoghurt during cold storage. Int. Dairy J., 17, 657-665.
Hernandez H. O., Muthaiyan A., Moreno F. J., Montilla A., Sanz M. L., Ricke S. C. (2012), Effect of prebiotic carbohydrates on the growth and tolerance of Lactobacillus. Food Microbiol., 30, 355-361.
Muzammil H. S., Rasco B., Sablani S. (2017), Effect of inulin and glycerol supplementation on physicochemical properties of probiotic frozen yogurt. Food Nutrition Res, 61(1), 1290314.
Tabasco R., Paarup T., Janer C., Pelaez C., Requena T. (2007), Selective enumeration and identification of mixed cultures of Streptococcus thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, L. acidophilus, L. paracasei subsp. paracaseiand Bifidobacteriumlactis in fermented milk. Int. Dairy J., 17, 1107–1114.
Tharmaraj N., Shah N. P. (2003), Selective Enumeration of Lactobacillus delbrueckiissp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and Propionibacteria. J. Dairy Sci., 86, 2288–2296.
Muzammil H. S., Javed I., Rasco B., Rashid A. (2015), Viability of probiotics in frozen yogurt with different levels of overrun and glycerol supplementation. Int. J. Agri. Biol., 17, 648‒652.
Ahmadi A., Milani E., Madadlou A., Mortazavi S. A., Mokarram R. R., Salarbashi D. (2014), Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide. J. Food Sci. Technol., 51(8), 1568-1574.
Akalin A. S., Erisir D. (2008), Effects of Inulin and Oligofructose on the Rheological Characteristics and Probiotic Culture Survival in Low-Fat Probiotic Ice Cream. J. Food Sci., 73, 184-188.
Cardarelli H. R., Buriti F. C. A., Castro I. A., Saad S. M. I. (2008), Inulin and oligofructose improve sensory quality and increase the probiotic viable count in potentially synbiotic petit-suisse cheese. Food Sci. Technol., 41 1037–1046.
Capela P., Hay T. K. C., Shah N. P. (2006), Effect of cryoprotectants, prebiotics and microencapsulation on survival of probiotic organisms in yoghurt and freeze dried yoghurt. Food Res. Int., 39, 203–211.
Pinto S. S., Freire C. B. F., Munoz I. B., Barreto P. L. M., Prudencio E. S., Amboni R. D. M. C. (2012), Effects of addition of microencapsulated Bifidobacterium BB-12 on the properties of frozen yogurt. J. Food Eng., 111, 563-569.
Rezaei R., Khomeiri M., Aalami M., Kashaninejad M. (2014), Effect of inulin on the physicochemical properties, flow behavior and probiotic survival of frozen yogurt. J. Food Sci. Technol., 51(10), 2809-2814.
Ranadheera C. S., Evansa C. A., Adamsa M. C., Baines S. K. (2013), Production of probiotic ice cream from goat’s milk and effect of packaging materials on product quality. Small Ruminant Res, 112, 174– 180.
Alamprese C., Foschino R., Rossi M., Pompei C., Savani L. (2002), Survival of Lactobacillus johnsonii La1 and influence of its addition in retailmanufactured ice cream produced with different sugar and fat concentrations. Int. Dairy J., 12, 201-208.
Haynes I. N., Playne M. J. (2002), Survival of probiotic cultures in low-fat ice-cream. Aus. J. Dairy Technol., 57(1), 10-14.
Jay J. M., Loessner M. J., Golden D. A. (2005), Modern Food Microbiology. 7th ed. Springer Science Business Media, Inc., N. Y., pp. 395-409.
Whelan A. P., Regand A., Vega C., Kerry J. P., Goff H. D. (2008), Effect of trehalose on the glass transition and ice crystal growth in ice cream. Int. J. Food Sci. Technol., 43, 510–516.