Isolation and Screening of Protease Enzyme Producing Bacteria from Cheese at Dilla University, Ethiopia
International Journal of Nutrition and Food Sciences
Volume 4, Issue 2, March 2015, Pages: 234-239
Received: Mar. 8, 2015; Accepted: Mar. 19, 2015; Published: Mar. 23, 2015
Views 3161      Downloads 415
Fekadu Alemu, Department of Biology, College of Natural and Computational Sciences, Dilla University, Dilla, Ethiopia
Article Tools
Follow on us
Enzymes are biological catalysts that facilitate the conversion of substrates into products by providing favorable conditions that lower the activation energy of the reaction. An enzyme may be a protein or a glycoprotein and consists of at least one polypeptide moiety. Proteases are the most important industrial enzymes and comprise about 25% of commercial enzymes in the world. Two third of the industrially produced proteases are from microbial sources. The current study was focused on the screening of protease enzyme producing bacteria from dairy product of cheese at Dilla University. Cheese sample was collected from Dilla town market. The proteases enzyme production bacteria were screened on milk agar plate through spread plate method. As the result, certain bacteria were screened for protease enzyme production as well as were confirmed on milk agar medium. Almost all isolated (35 isolate) of bacteria from cheese food stuff were had a potential in the production of protease enzyme which help for various activities. Therefore, all isolate had a promising potential for production proteolytic enzyme which are used in industries and the other sectors.
Cheese, Clear Zone, Enzyme, Proteases, Screening of Bacteria
To cite this article
Fekadu Alemu, Isolation and Screening of Protease Enzyme Producing Bacteria from Cheese at Dilla University, Ethiopia, International Journal of Nutrition and Food Sciences. Vol. 4, No. 2, 2015, pp. 234-239. doi: 10.11648/j.ijnfs.20150402.25
Aleksieva P, Peeva L (2000). Investigation of acid protinase biosynthesis by the fungus Humicola Lutea 120-5 in an airlift bioreactor. Enzyme Microb. Technol. 26: 402-405.
Benslimane C, Lebrihi A, Lounes A, Lefebvre G, Germain P (1995). Influnce of dextrins on the assimilation of yeast extract amino acids in culture of Streptomyces ambofaciens producer of spiramycin. Enzyme. Microb. Technol. 17: 1003-1013.
Bhaskar N, Sudeepa ES, Rashmi HN, Selvi AT (2007). Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresour. Technol. 98: 2758-2764.
Bigelow P, Wyman E (2004). Production of cellulolytic on bagasse pretreated with chemicals. Applied Biochem. Biotechnol. 102: 78-82.
Burhan A, Nisa U, Gokhan C, Ashabil A, Osmair G (2003). Enzymatic Properties of a novel thermostable thermophilic alkaline and chelator resistant amylase from an alkaphilic Bacillus sp isolate ANT-6. Process Biochemistry (38): 1397–1403.
Deng A, WU J, Zhang Y, Zhang G, Wen T (2010). Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus spp. B001. Bioresour. Technol. 101: 7100-7116.
Djamel C, Ali T, Nelly C (2009). European journal of Scientific Research. 25(3): 469-477.
Enzyme Technical Association (2001). Enzymes; A primer on use and Benefits today and tomorrow. Washington, DC 200036 pp. 1-32.
Folasade M, Olajuyigbe, Joshua O, Ajele (2005). Production dynamics of extracellular protease from Bacillus species. Afr. J. Biotechnol. 4(8): 776-779.
Gupta R, Beg QK, Khan S, Chauhan B (2002). An overview on fermentation, downstream processing and properties of microbial alkaline proteases. Appl. Microbiol. Biotechnol. 60(4): 381-395.
Gupta R, Beg QK, Lorenz P (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol. 59: 15-20.
Haq I, Mukhtar ZA, Riaz N (2004). Protease biosynthesis by mutant strain of Penicillium griseoroseum and cheese formation. Pakistan J. Biol. Sci. 7: 1473–1476.
Holt JG, Krieg NR, Sneath PHA, Stately JT, Williams ST (1994). Bergey’s manual of determinative bacteriology 9th ed. Williams and Wilkins: Baltimore.
Jellouli K, Bougatef A, Manni L, Agrebi R, Siala R, Younes I, Nasri M (2009). Molecular and biochemical characterization of an extracellular serine-protease from Vibrio etschnikovii. Microbiol. Biotechnol. 36: 939-948.
Muthulakshmi C, Gomathi D, Kumar DG, Ravikumar G, Kalaiselvi M, Uma C (2011). Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation. Jordan Journal of Biological Sciences 4(3): 137-148.
Nout MJR, Rombouts FM (1990). Recent developments in tempe research. J. App. Bacterial. 69: 609-633.
Oyeleke SB, Oduwole AA (2009). Production of amylase by bacteria isolated from a cassava dumpsite in minna, Nigerstate, Nigeria. African Journal of Microbiology Research 3(4): 143-146.
Pandey A, Soccol CR, Mitchell D (2000). New developments in solid state fermentation. Bioprocesses and products. Proc. Biochem. 35: 1153–1169.
Rao MB, Tanksale AM, Gha MS, Deshpande VV (1998). Molecular and biotechnological aspects of microbial proteases Microbiol. Mol Biol. Reviews 62(3): 597-635.
Sanchez-Porro C, Martın S, Mellado E, Ventosa A (2003). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of Applied Microbiology 94(2): 295–300.
Sharma OP, Sharma KD, Nath K (1980). Production of proteolytic enzyme by fungi. Rev. Roum. Biochem. 17: 209–215.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186