UV - Visible Spectrophotometric Quantification of Total Polyphenol in Selected Fruits
International Journal of Nutrition and Food Sciences
Volume 4, Issue 3, May 2015, Pages: 397-401
Received: Apr. 13, 2015; Accepted: Apr. 29, 2015; Published: May 21, 2015
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Authors
Terefe Tafese Bezuneh, Department of Chemistry, Arbaminch University, Arbaminch, Ethiopia
Eyob Mulugeta Kebede, Department of Chemistry, Arbaminch University, Arbaminch, Ethiopia
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Abstract
Fruits are known as a richest source of bioactive compounds as polyphenols which are known to have significant health promoting properties. The present study investigates the total polyphenol content of some selected fruits extracted in: acidified 70 % ethanol, acidified 70 % methanol, acidified 70 % acetone, and 100 % water solvents. Standard gallic acid solution prepared in the range of 50-500 µg/L was used to plot a calibration graph. A good linearcalibration graph (r= 0.998, n=3) was obtained by plotting absorbance at 511 nm versus standard solution and all results are given as gallic acid equivalent (GAE, mg/g, dry weight). The concentration of total polyphenols varies with the solvent used and also among different samples. Higher concentration was detected in papaya fruit both in the peel and pulp (238.6±3.64, 135.2±0.09; GAE, mg/g, dry weight) respectively and lower concentration in banana peel and pulp (43.2± 0.13, 26.6±0.06; GAE, mg/g, dry weight) respectively.
Keywords
Gallic Acid, Antioxidant, Total-Polyphenol
To cite this article
Terefe Tafese Bezuneh, Eyob Mulugeta Kebede, UV - Visible Spectrophotometric Quantification of Total Polyphenol in Selected Fruits, International Journal of Nutrition and Food Sciences. Vol. 4, No. 3, 2015, pp. 397-401. doi: 10.11648/j.ijnfs.20150403.28
References
[1]
Maliwan A, Wongduan S, Saowanee L, et al. (2013). Rapid Screening Method for Assessing Total Phenolic Content Using Simple Flow Injection System with Laccase based-biosensor. International Journal of Electrochemical Science, 8, 10526 - 10539.
[2]
Takako Y, Jeong S N, and Chan H P (2013). Green Tea Polyphenols for the Protection against Renal Damage Caused by Oxidative Stress. Hindawi Publishing Corporation, 1-11.
[3]
Maisarah A M, Asmah R and Fauziah O (2014). Proximate Analysis, Antioxidant and Antiproliferative Activities of Different Parts of Carica Papaya. Journal of Nutrition and Food Science, 4(2), 1-7.
[4]
Wei Q L, Jian G X (2014). Profile of DNA damage protective effect and antioxidant activity of different solvent extracts from the pericarp of Garciniamangostana. Journal of Food and Nutrition Sciences, 3(1), 1-6.
[5]
Chwan L S, Ming C C, and Jia S W (2013). Tea and bone health: steps forward in translational nutrition. American Journal of Clinical Nutrition, 98(l), 1694S-1699S.
[6]
Giuseppina PP L, Fabio V, Camila R C, et al. (2014). Polyphenols in Fruits and Vegetables and Its Effect on Human Health. Food and Nutrition Sciences, 5, 1065-1082.
[7]
Rajan M and Thangaraj P (2014). Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from OsbeckiaparvifoliaArn. – An in vitro approach. Journal of King Saud University Science, 26, 267-275.
[8]
Sahin K, Orhan C, Tuzcu M, et al. (2010). Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails. Poultry Science, 89, 2251–2258.
[9]
Cestmir C and Jitka C (2015). Oxidative Stress to the Cornea, Changes in Corneal Optical Properties, and Advances in Treatment of Corneal Oxidative Injuries. Hindawi Publishing Corporation, 1-10.
[10]
Jeanelle B and Rui H L (2004). Apple phytochemicals and their health benefits. Nutrition Journal, 3, 1-15.
[11]
Mohammed S, Hamzat I T, Bashir M A, et al. (2013). An overview of natural plant antioxidants: analysis and evaluation. Advances in Biochemistry, 1(4), 64-72.
[12]
Stefano P, Crispian S (2009). Polyphenols, oral health and disease: A review. Journal of dentistry, 37, 413-423.
[13]
Balz F and Jane V H (2003). Antioxidant Activity of Tea Polyphenols In Vivo: Evidence from Animal Studies. The Journal of Nutrition, 133, 3275S-3284S.
[14]
Nikita L and Chaitanya S (2012). Polyphenols and oral health. RSBO, 9(1), 74-84.
[15]
Palafox C H, Yahiab E, Islas O M, et al. (2012). Effect of ripeness stage of mango fruit (MangiferaindicaL.,cv.Ataulfo) on physiological parameters and antioxidant activity. ScientiaHorticulturae, 135, 7-13.
[16]
Massimo A, Carmela F, Roberta B, et al. (2007). Polyphenols, dietary sources and bioavailability. Ann InstitutoSuperiore di Sanita, 43(4), 348-361.
[17]
Alecia S M, Dai Q, Xiao O S, et al. (2003). Intake of Fruits, Vegetables and Selected Micronutrients in Relation To the Risk of Breast Cancer. International Journal of Cancer, 105, 413-418.
[18]
Constantine D S (2007). Extraction, separation, and detectionmethods for phenolic acids and flavonoids. Journal of Separation Science, 30, 3268-3295.
[19]
Claudine M, Augustin S, Christine M, et al. (2004). Polyphenols: food sources and bioavailability. American Journal of Clinical Nutrition, 79, 727-47.
[20]
Charles W H, Giselle M M, Manuel S V et al. (2012). Phenolic compounds in fruits–an overview. International Journal of Food Science and Technology, 47, 2023–2044.
[21]
Koffi E, Sea T, DodeheY,et al. (2010). Effect of solvent type on extraction of polyphenols from twenty three Ivorian plants. Journal of Animal and Plant Sciences, 5(3), 550 558.
[22]
Usune E, Alfredo F Q, Fermín I M, et al. (2013). Impact of Polyphenols and Polyphenol-Rich Dietary Sources on Gut Microbiota Composition. Journal of Agricultural and Food Chemistry, 61, 9517-9533.
[23]
Koen B, Ruth V, Guido V, et al. (2004). Induction of Cancer Cell Apoptosis by Flavonoids Is Associated with Their Ability to Inhibit Fatty Acid Synthase Activity. The Journal of Biological Chemistry, 280(7), 5636–5645.
[24]
Ali K, Meredith A W and Thomas H R (2013). Techniques for Analysis of Plant Phenolic Compounds. Molecules, 18, 2328-2375.
[25]
Nemanja M, Branko P, Olga M, et al. (2012). Phenolic content and antioxidant capacity of fruits of plum cv. ‘Stanley’ (Prunusdomestica L.) as influenced by maturity stage and on-tree ripening. Australian Journal of Crop Science, 6(4), 681-687.
[26]
Merlene A B, Suriyakala M A, and Gothandam K M (2012). Varietal Impact on Phytochemical Contents and Antioxidant Properties of Musa acuminata (Banana). Journal of Pharmaceutical Science and Research, 4(10), 1950-1955.
[27]
Oruma P, Prapasri P, Anadi N, et al. (2008). Changes of antioxidant activity and total phenolic compounds during storage of selected fruits. Journal of Food Composition and Analysis, 21, 241–248.
[28]
Joseph A V (2005). Polyphenols and cardiovascular disease: effects on endothelial and platelet function. American Journal of Clinical Nutrition, 81(l), 292S–7S.
[29]
David V, Ana R M, Giulia C, et al. (2010). Polyphenols and Human Health: Prevention of Disease and Mechanisms of Action. Nutrients, 2, 1106-1131.
[30]
Moushumi L C, Santosh K K, Rajiv R M, et al. (1999). A Flavonoid Antioxidant, Silymarin, Affords Exceptionally High Protection against Tumor Promotion in the Sencar Mouse Skin Tumorigenesis Model. Cancer Research, 59, 622–632.
[31]
Ilja C A and Peter C H (2005). Polyphenols and disease risk in epidemiologic studies. American Journal of Clinical Nutrition, 81(l), 317S–25S.
[32]
Patricia G S, Aranzazu M S, Antonio S C, (2010). Phenolic Compound Extraction Systems for Fruit and Vegetable Samples. Molecules, 15, 8813-8826.
[33]
Lizbeth A C, Jemina T C, Angel M M, et al. (2014). Antioxidant Capacity and Total Phenolic Content in Fruit Tissues from Accessions of Capsicum chinenseJacq. (Habanero Pepper) at Different Stages of Ripening. Hindawi Publishing Corporation, 1-5.
[34]
Carolina H, Sergio A, Italo C, et al. (2010). Determination of Antioxidant Capacity, Total Phenolic Content and Mineral Composition of Different Fruit Tissue of Five Apple Cultivars Grown in Chile. Chilean Journal of Agricultural Research, 70(4), 523-536.
[35]
David O K (2014). Polyphenols and the Human Brain: Plant “Secondary Metabolite” Ecologic Roles and Endogenous Signaling Functions Drive Benefits. Advanced in Nutrition, 5, 515–533.
[36]
Manmohan S and Purnima R (2013). Antioxidant Activity, Total Flavonoid and Total Phenolic Content of Musa acuminate Peel Extracts. Global Journal of Pharmacology, 7 (2), 118-122.
[37]
Rong T (2010). Chemistry and Biochemistry of Dietary Polyphenols. Nutrients, 2, 1231-1246.
[38]
Mônica G S, Cecilia V N, Horacio D M (2013). A new method for quantification of total polyphenol content in medicinal plants based on the reduction of Fe(III)/1,10-phenanthroline complexes. Advances in Biological Chemistry, 3, 525-535.
[39]
Hana K, Jeong Y M, Hyeonji K et al. (2010). Antioxidant and anti-proliferative activities of mango (Mangiferaindica L.) flesh and peel. Food Chemistry, 121, 429-436.
[40]
Fatemeh S R, Saifullah R, Abbas F M A et al. (2012). Total phenolics, flavonoids and antioxidant activity of banana pulp and peel flours: influence of variety and stage of ripeness. International Food Research Journal, 19 (3), 1041-1046.
[41]
Michalak A (2006). Phenolic Compounds and Their Antioxidant Activity in Plants Growing under Heavy Metal Stress. Polish Journal of Environmental Study, 15(4), 523-530.
[42]
Andressa B, Gisely C L and João C P M (2013). Application and Analysis of the FolinCiocalteu Method for the Determination of the Total Phenolic Content from LimoniumBrasiliense L. Molecules, 18, 6852-6865.
[43]
Shizuo T (2011). Polyphenol Content and Antioxidant Effects in Herb Teas. Chinese Medicine, 2, 29-31.
[44]
Jin D and Russell J M (2010). Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules, 15, 7313-7352.
[45]
Joshua D L, Jungil H, Guang-yu Y, et al. (2005). Inhibition of carcinogenesis by polyphenols: evidence from laboratory investigations. American Journal of Clinical Nutrition, 81(suppl), 284S-91S.
[46]
Nguyen N T, Phan P H, Huynh N O (2015). Optimizing the extraction conditions of phenolic compounds from fresh tea shoot. Journal of Food and Nutrition Sciences, 3(1-2), 106-110.
[47]
Dam S M (2015). Study on the extraction of polyphenol from Artocarpusaltilis with ultrasonic wave technology optimized by central composite design-response surface method. Journal of Food and Nutrition Sciences, 3(1-2), 115-118.
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