International Journal of Nutrition and Food Sciences

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Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil

Received: 17 April 2013    Accepted:     Published: 02 May 2013
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Abstract

The present investigation reports the characterization of the bacteriocin produced by the probiotic strain Lac-tobacillus plantarum 29V isolated from raw cow milk in the Western’s highlands of Cameroon, as well as the viability of this strain in the palm kernel oil. The antimicrobial compound synthesized by Lactobacillus plantarum 29V was sensitive to some proteolytic enzymes. It showed remarkable stability at high temperatures and in the presence of organic solvents, detergents and surfactants. It was also active in pH 2.0–10 and NaCl range of 1-7%. The neutralized cell-free supernatant of this bacterium inhibited the growth of several Lactobacillus spp., pathogenic and food spoilage microorganisms. The results of this study showed that palm kernel oil maintains the viable cell numbers of the probiotic strain Lactobacillus plantarum 29V, without any changes of peroxide and acid indexes of palm kernel oil.

DOI 10.11648/j.ijnfs.20130203.12
Published in International Journal of Nutrition and Food Sciences (Volume 2, Issue 3, May 2013)
Page(s) 102-108
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bacteriocin, Probiotic, Lactobacillus Plantarum, Palm Kernel Oil

References
[1] Galvez, R. Lucas-Lopez and H. Abriouel, "Application of bacteriocins in the control of foodborne pathogenic and spoilage bacteria". Critical Reviews in Biotechnology, 2008, 28: 125–152.
[2] L. Cocolin, R. Foschino, G. Comi and M.G. Fortina, "De-scription of the bacteriocins produced by two strains of En-terococcus faecium isolated from Italian goat milk". Food Microbiology, 2007, 31: 753-758.
[3] M.M.A. Lemos, A.C. Dias and G.L.S. de Castro Ferreira, "Inhibition of vancomycin and high-level aminoglyco-side-resistant enterococci strains and Listeria monocytogenes by bacteriocin-like substance produced by Enterococcus faecium E86". Current Microbiology, 2008, 57: 429–436.
[4] S.F. Deraz, E.N. Karlsson, M. Hedstrom, M.M. Andersson and B. Mattiasson, "Purification and characterisation of acidocin D20079, a bacteriocin produced by Lactobacillus acidophilus DSM 20079". Journal of Biotechnology , 2005, 117:343-354.
[5] R.D.S.C. Ranadheera, S.K. Baines and M. C. Adams, "Im-portance of food in Probiotic Efficacy". Food Research In-ternational, 2010, 43: 1-7.
[6] D. Granato, G.F. Branco, F. Nazzaro, A.G. Cruz and J.A.F. Faria, "Functional Food and Nondairy Probiotic Food De-velopment: Trends, Concepts and Products. Comprehensive" Reviews in Food Science and Food Safety, 2010, 9: 292-30.
[7] Y. Rivera-Espinoza, and Y. Gallardo-Navarro, "Non-dairy probiotic products. Food Microbiology, 2010, 27: 1–11.
[8] Kapseu, C. Tchiegang, J. Dellacherie and M.Dirand, Huiles noix de coco de palmiste cameroun : sources d'acide gras laurique. sostanze grasse, 1997, 74 : 205-7.
[9] D.V. Sieladie, N.F. Zambou, A. Cresci, A.F. Fonteh, P.M. Kaktcham and M.F. Tchouanguep, "Probiotic properties of lactobacilli strains isolated from raw cow milk in the western highlands of Cameroon". Innovative Romanian Food Bio-technology, 2011, 9: 12-28.
[10] S.D. Torodov, and L.M.T. Dicks, "Lactobacillus plantarum isolated from molasses produces bacteriocins active against Gram-negative bacteria". Enzyme and Microbial Technology, 2005, 36: 318-326.
[11] S.R. Biswas, P. Ray, M.C. Johnson and B. Ray, "Influence of growth conditions on the production of a bacteriocin, pediocin ACH, by Pediococcus acidilactici H. Applied Environmental Microbiology, 1991, 57: 1265–1267.
[12] S.K. Tiwari, and S. Srivastava, "Characterization of a Bac-teriocin from Lactobacillus plantarum Strain LR/14". Food Biotechnology, 2008, 22: 247 – 261.
[13] T. Ignatova, I. Iliev, N. Kirilov, T. Vassileva, M. Dalgalarrondo, T. Haertle, J.M. Chobert and I. Ivanova, "Effect of oligosaccharides on the growth of Lactobacillus delbrueckii subsp. bulgaricus strains isolated from dairy products". Journal of Agricultural and Food Chemistry, 2009, 57: 9496–9502.
[14] AFNOR, "Recueil des normes Françaises des corps gras, graines oléagineuses, produits dérivés". 2e Ed. AFNOR, Paris, 1981, 438p.
[15] S.D. Todorov, C.A. Van Reenen and L.M.T. Dicks, "Opti-mization of bacteriocin production by Lactobacillus plantarum ST13BR, a strain isolated from barley beer". Journal of General Applied Microbiology, 2004, 50: 149–157.
[16] N. Mojgani, , G. Sabiri, M. Ashtiani, and M. Torshizi,. "Characterization of bacteriocins produced by L. brevis NM 24 and L. fermentum NM 332 isolated from green olives in Iran". The Internet Journal of Microbiology. 2009, 6 (2).
[17] B.V. Fricourt, S.F. Barefoot, R.F. Testin and S.S. Hayasaka, "Detection and activity of plantaricin F an antibacterial sub-stance from Lactobacillus plantarum BF001 isolated from processed channel catfish". Journal of Food Protection, 1994, 57: 698-702.
[18] L. Saavedra, C. Minahk, A.P. Holgado and F. Sesma, "En-hancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence". Antimi-crobiological Agents Chemotherapies, 2004, 48: 2778-278.
[19] S.T. Ogunbanwo, A. Sanni and A. Onilude, "Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis". African Journal of Biotechnology, 2003, 2: 219-227.
[20] Hernández, E. Cardell and V. Zárate, "Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711". Journal of Applied Microbiology, 2005, 99: 77-84.
[21] M. De Kwaadsteniet, S.D. Todorov, H. Konoetze and L.M.T Dicks, "Characterization of a 3944 Da bacteriocin, produced by Enterococcus mundtii ST15, with activity against gram-positive and gram-negative bacteria". International Journal of Food Microbiology, 2005,105: 433–444.
[22] K. Kailasapathy, "Microencapsulation of Probiotic Bacteria: Technology and Potential Applications". Current Issues in Intestinal Microbiology, 2002, 3:39-48.
[23] J.D. Oliver, "The viable but nonculturable state in bacteria". The Journal of Microbiology, 2005, 43: 93-100.
[24] M.V. De Guchte, P. Serror, C. Chervaux, T. Smokvina, S. Ehrlich and E. Maguin,. "Stress responses in lactic acid bacteria". Antonie Van Leeuwenhoek, 2002, 82: 187–21.
Author Information
  • Laboratory of Biochemistry, Food Science and Nutrition (LABPMAN) - Department of Biochemistry - Faculty of Science - University of Dschang, Cameroon

  • Laboratory of Biochemistry, Food Science and Nutrition (LABPMAN) - Department of Biochemistry - Faculty of Science - University of Dschang, Cameroon

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    Zambou Ngoufack François, Kaktcham Pierre Marie, Tiogo Azemfack Huguette Noëlle, Guetiya Wadoum Raoul Emeric. (2013). Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil. International Journal of Nutrition and Food Sciences, 2(3), 102-108. https://doi.org/10.11648/j.ijnfs.20130203.12

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    ACS Style

    Zambou Ngoufack François; Kaktcham Pierre Marie; Tiogo Azemfack Huguette Noëlle; Guetiya Wadoum Raoul Emeric. Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil. Int. J. Nutr. Food Sci. 2013, 2(3), 102-108. doi: 10.11648/j.ijnfs.20130203.12

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    AMA Style

    Zambou Ngoufack François, Kaktcham Pierre Marie, Tiogo Azemfack Huguette Noëlle, Guetiya Wadoum Raoul Emeric. Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil. Int J Nutr Food Sci. 2013;2(3):102-108. doi: 10.11648/j.ijnfs.20130203.12

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  • @article{10.11648/j.ijnfs.20130203.12,
      author = {Zambou Ngoufack François and Kaktcham Pierre Marie and Tiogo Azemfack Huguette Noëlle and Guetiya Wadoum Raoul Emeric},
      title = {Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {2},
      number = {3},
      pages = {102-108},
      doi = {10.11648/j.ijnfs.20130203.12},
      url = {https://doi.org/10.11648/j.ijnfs.20130203.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijnfs.20130203.12},
      abstract = {The present investigation reports the characterization of the bacteriocin produced by the probiotic strain Lac-tobacillus plantarum 29V isolated from raw cow milk in the Western’s highlands of Cameroon, as well as the viability of this strain in the palm kernel oil. The antimicrobial compound synthesized by Lactobacillus plantarum 29V was sensitive to some proteolytic enzymes. It showed remarkable stability at high temperatures and in the presence of organic solvents, detergents and surfactants. It was also active in pH 2.0–10 and NaCl range of 1-7%. The neutralized cell-free supernatant of this bacterium inhibited the growth of several Lactobacillus spp., pathogenic and food spoilage microorganisms. The results of this study showed that palm kernel oil maintains the viable cell numbers of the probiotic strain Lactobacillus plantarum 29V, without any changes of peroxide and acid indexes of palm kernel oil.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Antimicrobial Activity of a Bacteriocin Produced by Lactobacillus Plantarum 29V and Strain’S Viability in Palm Kernel Oil
    AU  - Zambou Ngoufack François
    AU  - Kaktcham Pierre Marie
    AU  - Tiogo Azemfack Huguette Noëlle
    AU  - Guetiya Wadoum Raoul Emeric
    Y1  - 2013/05/02
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ijnfs.20130203.12
    DO  - 10.11648/j.ijnfs.20130203.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 102
    EP  - 108
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20130203.12
    AB  - The present investigation reports the characterization of the bacteriocin produced by the probiotic strain Lac-tobacillus plantarum 29V isolated from raw cow milk in the Western’s highlands of Cameroon, as well as the viability of this strain in the palm kernel oil. The antimicrobial compound synthesized by Lactobacillus plantarum 29V was sensitive to some proteolytic enzymes. It showed remarkable stability at high temperatures and in the presence of organic solvents, detergents and surfactants. It was also active in pH 2.0–10 and NaCl range of 1-7%. The neutralized cell-free supernatant of this bacterium inhibited the growth of several Lactobacillus spp., pathogenic and food spoilage microorganisms. The results of this study showed that palm kernel oil maintains the viable cell numbers of the probiotic strain Lactobacillus plantarum 29V, without any changes of peroxide and acid indexes of palm kernel oil.
    VL  - 2
    IS  - 3
    ER  - 

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