Combined Effect of Irradiation and Frozen Storage on Survival of Viable Bacteria and Inoculated Escherichia Coli in Chicken
Journal of Food and Nutrition Sciences
Volume 2, Issue 3, May 2014, Pages: 53-57
Accepted: Apr. 28, 2014; Published: May 10, 2014
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Authors
Wellington Torgby-Tetteh, Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Ghana
Abraham Adu-Gyamfi, Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Ghana
Bernard Tawiah Odai, Radiation Technology Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, Ghana
Victoria Appiah, Department of Nuclear Agriculture and Radiation Processing, School of Nuclear and Allied Sciences, University of Ghana, Ghana
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Abstract
Combined effect of irradiation and frozen storage on viable bacteria and inoculated Eschericia coli in chicken was investigated. Samples of uninoculated chicken and samples of chicken inoculated with E. coli were irradiated using a Co-60 source at doses of 0, 2 ,4,6 and 8 kGy and stored for 0, 7, 14, 21, 28, 35, 42, 49 and 56 days at -180 C. Samples were analyzed each week to enumerate surviving viable bacteria and E. coli. Irradiation doses of 2, 4, 6, and 8 kGy respectively reduced the population of viable bacteria in the uninoculated chicken by 2.06, 2.96, 3.91 and 4.21 log cycles. Storage for 56 days reduced populations of viable bacteria by approximately 2 log cycles for all irradiated uninoculated samples. Dose of 2 kGy reduced the population of E. coli in the unirradiated sample by 2.69 log cycles and 4, 6, 8 kGy reduced the population by > 7 log cycles. Storage for 56 days reduced the population of E. coli by 4.07 and > 3.52 log cycles respectively in the unirradiated and irradiated (2 kGy) samples. Irradiation doses of 4 to 8 kGy in combination with frozen storage were effective in reducing the populations of viable indigenous bacteria in addition to eliminating inoculated E. coli from chicken thus extending the shelf life and improving the hygienic quality.
Keywords
Chicken, Gamma Irradiation, Frozen Storage, Viable Bacteria, E. coli
To cite this article
Wellington Torgby-Tetteh, Abraham Adu-Gyamfi, Bernard Tawiah Odai, Victoria Appiah, Combined Effect of Irradiation and Frozen Storage on Survival of Viable Bacteria and Inoculated Escherichia Coli in Chicken, Journal of Food and Nutrition Sciences. Vol. 2, No. 3, 2014, pp. 53-57. doi: 10.11648/j.jfns.20140203.11
References
[1]
Adu-Gyamfi, A., Torgby-Tetteh, W., and Appiah, V. (2012) Microbiological Quality of Chicken Sold in Accra and Determination of D10-Value of E. coli, Food and Nutrition Sciences, 2012, 3, 693-698
[2]
Aymerich, T., Picouet, P. A., Monfort, J. M. (2008) Decontamination technologies for meat products. Meat Science, 78, 114-129
[3]
Mead, P. S., L. Slutsker, V. Dietz, L. F. McCaig, J. S. Bresee, C. Shapiro, P. M. Griffin, and R. V. Tauxe. (1999) “Food-Related Illness and Death in the United States,” Emerging Infectious Diseases, Vol. 5, No. 5, pp. 607-25.
[4]
USDA, (1996) U.S. Department of Agriculture, Food Safety and Inspection Service. Supplement - Final Regulatory Impact Assessment for Docket No. 93-016F, Pathogen Reduction; Hazard Analysis and Critical Control Point (HACCP) Systems: Final Rule,” Federal Register. Vol. 61, No. 144, July 25, 1996, pp. 38945-89.
[5]
Adu-Gyamfi, A., Nketsia-Tabiri, J and Apea Bah, F. (2008). Radiosensitivities of bacterial isolates on minced chicken and poached chicken meal and their elimination 77, (2):174-178.
[6]
Thayer, D. W. (1993). Extending shelf life of poultry and red meat by irradiation processing, Journal of Food Protection 56(10): 831-833
[7]
Spoto, M. H. F., Gallo, C. R., Alcarde, A. R., Gungel, Silvia do Amaral. M., Blumer, L., Walder, M. M. and Do-marco, R. E. (2000). Gamma irradiation in the control of pathogenic bacteria in refrigerated chicken meat. Scientia Agricola, 57(3): 389-394.
[8]
Oraei, M., Motalebi, A. A., Hoseini, E and Javans, S. (2010). Effect of gamma irradiation and frozen storage on the microbial quality of Rainbow trout (Oncorhynchus mykiss) fillet. Iranian Journal of Fisheries Sciences, 10(1), 76-84
[9]
Nunticha, T., Pattama, R,. and Pongtep W. (2011). Radiation resistances and de-contamination of common pathogenic bacteria contaminated in white scar oyster (Crassostrea belcheri) in Thailand. Radiation Physics and Chemistry, 80, 828-832
[10]
Cutrubinis C. D., Sa-vua, D., Elisabeta S. C., Mihaia R., Secub Ponatta C., (2007). Preliminary Study of on detection of irradiated foodstuffs from the Romanian market., Radiation Physics and Chemistry, 76, 1450-1454
[11]
Farkas, J., 2006. Irradiation for better foods. Trends in Food Science & Tech-nology 17, 148–152.
[12]
Kim, H. J., Ham, J. S., Lee, J. W., Kim, K., Ha, S. D., Jo, C., 2010. Effects of gamma and electron beam irradiation on the survival of pathogens inoculated into sliced and pizza cheeses. Radiation Physics and Chemistry 79, 731–734.
[13]
Kanatt, S.R., Chander, R., Sharma, A.: Effect of radiation processing on the quality of chilled meat products. Meat Sci., 2005; 69: 269-275.
[14]
Yoon K. S. (2003) Effect of gamma irradiation on the texture and microstructure of chicken meat, Meat science, 63, 273-277
[15]
Jo, C., Lee, N.Y., Kang, H.J., Shin, D.H., Byun, M.W. (2004). Inactivation of foodborne pathogens in marinated beef rib by ionizing radiation. Food Microbiol. 21: 543-548
[16]
Lacroix, M., Quattara, B. (2000). Combined industrial processes with irradiation to assure innocuity and preservation of food products-a review. Food Res Int. 33(9), 719–724.
[17]
International Consultative Group on Food Irradiation (1999). Irradiation and Trade in food and Agricultural Products, ICGFI Secretariat, ICGFI Policy Document.
[18]
Farkas, J. (1998). Microbiology safety of foods, International Journal of Microbiology 9:1-15
[19]
Clark, P. J (2006). Food irradiation update, Food Technology Magazine, 60(10), 73.
[20]
Diehl, J.F. (1995). Safety of Irradiated Foods (Second Edition). Marcel Dekker, Inc., New York
[21]
Smith, J. S. and Pillai, S. (2004) Irradiation and Food Safety. An IFT Scientific Status Summary, Food Technology. 58 (11): 48-55
[22]
Niemira, B.A. and C.H. Sommers (2006), New Applications in Food Irradiation. In D.R. Heldman, ed., Encyclopedia of Agricultural, Food and Biological Engineering, Dekker Encyclopedias, Taylor & Francis Publishers.
[23]
Diehl, J. F. (1990) Biological Effects of Ionizing Radiation, in: Diehl, J. F. (Eds)., Safety of Irradiated Foods, Marcel Dek-ker Inc., New York, 1990, pp. 95-136.
[24]
Gomes, H. A., Nepomuceno da Silva, E., Cardello, H. M. A. B., Cipolli, K. M. V. B. (2003). Effect of gamma radiation on refrigerated mechanically deboned chicken meat quality, Meat Science, 65, 919–926
[25]
Yazdia F. T. and Joukib M. (2012). Gamma irradiation effects on microbial decontamination of ostrich meat, Scientific Journal of Microbiology 1(5) 119-125
[26]
Thayer, D. W. (2000). Sources of Variation and Uncertainty in the Estimation of Radiation D-10 Values for Foodborne Pathogens, ORACBA News, Vol 4 No.5
[27]
Monk, J. D., Beuchat, L. R. and Doyle, M. P. (1995). Irradiation Inactivation of Food-Borne Microorganisms, Journal of Food Protection, Vol. 58, No. 2, 1995, pp. 197-208
[28]
Thayer, D.W. (1995). Use of irradiation to kill enteric pathogens on meat and poultry. Journal of Food Safety, 15: 181-192.
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