Assessment of AflatoxinM1and Enteropathogenic Microorganism Levels in Milk Samples Vended in Cross River State, Nigeria
Journal of Food and Nutrition Sciences
Volume 2, Issue 3, May 2014, Pages: 81-86
Accepted: Apr. 28, 2014; Published: Jun. 10, 2014
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Authors
Peters Henry, Biochemistry Department, University of Calabar, Cross River State, Nigeria
Christine Emmanuel-Ikpeme, Biochemistry Department, University of Calabar, Cross River State, Nigeria
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Abstract
Aflatoxin M1 and enteropathogenic microorganism levels in milk samples vended in Cross River State were investigated. Thirty one milk samples were purchased from supermarkets and markets across the three (3) senatorial districts of Cross River State during August to November 2012. The milk samples were grouped into three (3) categories; A (Evaporated milk samples), B (Powdered milk samples), C (Infant milk formula). The milk samples were analyzed for aflatoxin M1 (AFM1) by competitive enzyme linked immunosorbent assay (ELISA) while enteropathogens (Salmonella, Escherichia coli, Mould, Mesophilic Aerobic bacteria and Coliform) were cultured for microbiological sensitivity test using standard methods. Aflatoxin M1 (AFM1) was found in 100 percent of all the milk samples that were analyzed in this study. The contamination levels ranged from 0.06µg/l to 0.07g/l, while the mean value was 0.07µg/l. There were no significant differences (P>0.05) between the mean concentrations of AFM1 of the milk samples among the different categories. All the different milk samples (100%) exceeded the European Union maximum acceptable levels (0.05µg/l). None of the milk samples exceeded the Nigerian permissible limit (0.5µg/l). Salmonella, Escherichia Coli and Coliform bacteria were not detected in the milk samples. Aerobic Mesophilic bacteria and Mould were present in the milk samples but did not exceed the standard of 105cfu/ml for aerobic mesophilic bacteria and 102cfu/ml for mould. It is concluded that the milk samples vended in Cross River State contain aflatoxin M1 and detectable enteropathogen levels which fall within Nigerian regulatory limits.
Keywords
Aflatoxin M1, Pasteurized Milk, Measurement, Enteropathogens, Elisa, Nigeria
To cite this article
Peters Henry, Christine Emmanuel-Ikpeme, Assessment of AflatoxinM1and Enteropathogenic Microorganism Levels in Milk Samples Vended in Cross River State, Nigeria, Journal of Food and Nutrition Sciences. Vol. 2, No. 3, 2014, pp. 81-86. doi: 10.11648/j.jfns.20140203.16
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