Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey
Two hundred sixty one samples, collected from supermarkets in Istanbul, Turkey. All samples were analysed for aerobic mesophilic bacterial counts (AMC), aerobic psychrotrophic bacterial counts (APC), enumeration of yeasts and moulds (YM), coliforms, Escherichia coli and detection of Escherichia coli O157:H7, Salmonella spp., Listeria monocytogenes, thermotolerant Campylobacter spp. AMC ranged from 2.95 to 3.75 log10 CFU/g. APC ranged from 0 to 3.55 log10 CFU/g. The highest counts of coliforms were found in carrot, spinach, green leaf lettuce, cos lettuce and iceberg lettuce. The highest counts of YM were found in tomato, spinach, green leaf lettuce, cos lettuce and iceberg lettuce. Green leaf lettuces, cos lettuces, iceberg lettuces, spinach and carrot were highly contaminated with aerobic mesophilic (3.6 log10 CFU/g), psychrotrophic microorganisms (3.4 log10 CFU/g), and showed a high incidence of E.coli (41.77% of samples). Of the samples analysed, 10 (3.83%) were Salmonella spp. positive and 17 (6.51%) were thermotolerant Campylobacter spp. positive. None of the samples was positive for Escherichia coli O157:H7 and Listeria monocytogenes. Fresh vegetables and fruits, sold in Istanbul, Turkey, are needed to control foodborne pathogens especially Salmonellosis and Campylobacteriosis.
Serkan Kemal Buyukunal,
Microbiological Quality of Fresh Vegetables and Fruits Collected from Supermarkets in Istanbul, Turkey, Journal of Food and Nutrition Sciences.
Vol. 3, No. 4,
2015, pp. 152-159.
Abadias M, Usall J, Anguera M, Solsona C, Vinas I. Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. Int. J. Food Microbiol., 2008; 123: 121-129.
Anonymous. Diet, nutrition and the prevention of chronic diseases. Report of a Joint FAO/WHO Expert Consultation, World Health Organization, Geneva. Available at: http://www.fao.org/DOCREP/005/AC911E/AC911E00.HTM, 2004.
Beuchat LR. Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes Infect., 2002; 4: 413-423.
Tournas VH. Moulds and yeasts in fresh and minimally processed vegetables, fruits, and sprouts. Int. J. Food Microbiol., 2005; 99: 71-77.
Berger CN, Sodha SV, Shaw RK, Griffin PM, Pink D, Hand P, Frankel G. Fresh fruit and vegetables as vehicles for the transmission of human pathogens. Environ. Microbiol., 2010; 12(9): 2385-2397.
Food and Drug Administration (FDA). Analysis and evaluation of prevention control measures for the control and reduction/elimination of microbial hazardson fresh and fresh-cut produce, Center for Food Safety and Applied Nutrition. Available at:http://www.fda.gov/Food/FoodScienceResearch/ SafePracticesforFoodProcesses/ucm091016.htm. 2001.
Zhao Y. Pathogens in fruit. In: Jonger, W. (Eds.), Improving the safety of fresh fruit and vegetables. CRC Press, Washington, DC, 2005, pp. 44-88.
World Health Organisation/Food and Agriculture Organization (WHO/FAO). Microbiological hazards in fresh leafy vegetables and herbs, Microbiological risk assessment series, Meeting Report. 20, Avenue Appia CH-1211 Geneva 27, Switzerland. Available at: http://www.fao.org/ag/agn/agns/jemra/ Jemra_Report%20on%20fresh%20leafy%20vegetables%20and%20herbs.pdf. 2008.
Sela S, Fallik E. Microbiological Quality and Safety of Fresh Produce. In: Florkowski WJ, Shewfelt RL, Brueckner B, Prussia SE. (Eds.), Postharvest Handling. Academic Press Inc, Philadelphia, PA, 2009. p.351-398.
Aycicek H, Oguz U, Karci K. Determination of total aerobic and indicator bacteria on some raw eaten vegetables from wholesailers in Ankara, Turkey. Int. J. Hyg. Environ. Health., 2006; 209: 197-201.
Buck JW, Walcott RR, Beuchat LR. Recent trends in microbiological safety of fruits and vegetables. Plant Health Progress DOI: 10.1094/PHP-2003-0121-01-RV (electronic journal), 2003.
Brackett RE. Incidence, contributing factors, and control of bacterial pathogens in produce. Postharvest Biol. Tec., 1999; 15: 305-311.
Warriner K, Huber A, Namvar A, Fan W, Dunfield K. Recent advances in the microbial safety of fresh fruits and vegetables. Adv. Food Nutr. Res., 2009; 57: 155-208.
Viswanathan P, Kaur R. Prevalance and growth of pathogens on salad vegetables, fruits, and sprouts. Int. J. Hyg. Envir. Heal., 2001; 203: 205-213.
Oliveira M, Usall J, Vinas I, Anguera M, Gatius F, Abadias M. Microbiological quality of fresh fresh lettuce from organic and conventional production. Food Microbiol., 2010; 27: 679-684.
Gemmell ME, Schmidt S. Microbiological assessment of river water used for the irrigation of fresh produce in a sub-urban community in Sobantu, South Africa. Food Res. Int., 2012; 47: 300-305.
Badosa E, Trias R, Pares D, Pla M, Montesinos E. Microbiological quality of fresh fruit and vegatable products in Catalonia (Spain) using normalised plate-counting methods and real time polymerase chain reaction (QPCR). J. Sci. Food Agr., 2008; 88: 605-611.
Gunasena DK, Kodikara CP, Ganepola K, Widanaparthirana S. Occurrence of Listeria monocytogenes in food in Sri Lanka. J. Nat. Sci. Council, 1995; 23: 107-114.
Johnston LM, Jaykus LA, Moll D, Martinez MC, Anciso J, Mora B, Moe CL. A field study of the microbiological quality of fresh produce of domestic and Mexican origin. Int. J. Food Microbiol., 2006; 112: 83-95.
Kumar A, Agarwal RK, Bhilegaonkar KN, Shome BR, Bachhil VN. Occurence of Campylobacter jejuni in vegetables. Int. J. Food Microbiol., 2001; 67: 153-155.
Verhoeff-Bakkenes L, Jansen HAPM, in’t Veld PH, Beumer RR, Zwietering MH, van Leusden FM. Consumption of raw vegetables and fruits: A risk factor for Campylobacter infections. Int. J. Food Microbiol., 2011; 144: 406-412.
Erkan ME, Vural A. Investigation of microbial quality of some leafy green vegetables. J. Food Technol., 2008; 6(6): 285-288.
Seow J, Agoston R, Phua L, Yuk HG. Microbiological quality of fresh vegetables and fruits sold in Singapore. Food Control, 2012; 25: 39-44.
Abadias M, Alegre I, Oliveira M, Altisent R, Vinas I. Growth potential of Escherichia coli O157:H7 on fresh-cut fruits (melon and pineapple) and vegetables (carrot and escarole) stored under different conditions. Food Control, 2012; 27: 37-44.
Allen KJ, Kovacevic J, Cancarevic A, Wood J, Xu J, Gill B, Allen JK, Mesak LR. Microbiological survey of imported produce available at retail across Canada. Int. J. Food Microbiol., 2013; 162: 135-142.
Johnston LM, Jaykus LA, Moll D, Martinez MC, Anciso J, Mora B, Moe CL. A field study of the microbiological quality of fresh produce. J. Food Protect., 2005; 68(9): 1840-1847.
Lemeilleur S, Codron JM. Marketing cooperative vs. commission agent: The Turkish dilemma on the modern fresh fruit and vegetable market. Food Policy, 2011; 36: 272-279.
Anonymous. The results of address based population registration system. The Turkish Statistical Institute. Available at: http://www.turkstat.gov.tr/ PreHaber Bultenleri. do?id=13425, 2013.
Food and Drug Administration (FDA). Bacteriological Analytical Manual [online](8th edition.). Available at: http://www.fda.gov/Food/ScienceResearch/ LaboratoryMethods/BacteriologicalAnalyticalManualBAM/default.htm, 1998.
Anonymous. Microbiology of food and animal feeding stuffs—Horizontal methods for the enumeration of microorganisms. Colony-count technique at 30 °C.. ISO 4833:2003, International Organization for Standardization, Geneva, Switzerland, 2003.
Anonymous. Microbiology of food and animal feeding stuffs—Horizontal methods for the enumeration of psychrotrophic microorganisms. ISO 17410:2001, International Organization for Standardization, Geneva, Switzerland, 2001b.
Anonymous. Microbiology of food and animal feeding stuffs-Enumeration of yeasts and mould. ISO 7954:1987, International Organization for Standardization, Geneva, Switzerland, 1987.
Frampton EW, Restaino L, Blaszko N. Evaluation of the β-glucuronidasesubstrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC) in a 24-hour direct plating method for Escherichia coli. J. Food Protect., 51(5): 402-404, 1988.
Andrews W. Manual of food quality control 4. Chapter 3, Microbiological Analysis (rev. 1). Food and Drug Administration, FAO Consultant, Washington, DC, 1992.
Anonymous. Microbiology of Food and Animal Feeding stuffs—Horizontal Method for the Detection of Escherichia coli O157. ISO 16654:2001, International Organization for Standardization, Geneva, Switzerland, 2001a.
Anonymous. Microbiology of Food and Animal Feeding Stuffs—Horizontal Method for the Detection of Salmonella spp. ISO 6579:2002, International Organization for Standardization, Geneva, Switzerland, 2002.
Anonymous. Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of Listeria monocytogenes. ISO 11290, International Organization for Standardization, Geneva, 1996.
Anonymous. Microbiology of Food and Animal feeding Stuffs-Horizontal Method for Detection and Enumeration of Campylobacter spp. ISO 10272-1:2006, International Organisation for Standardisation, International Organization for Standardization, Geneva, Switzerland, 2006.
Lund M, Nordentoft S, Pedersen K, Madsen M. Detection of Campylobacter spp. in chicken fecal samples by real-time PCR. J. Clin. Microbiol., 2004; 42: 5125–5132.
Pianetti A, Sabatini L, Citterio B, Pierfelici L, Ninfali P, Bruscolini F. Changes in microbial populations in ready-to-eat vegetable salads during shelflife. Ital. J. Food Sci., 20: 245-254, 2008.
Anonymous. HACCP-TQM Technical guidelines. Section IV Contamination levels and microbiological control. Available at: http://www.hi-tm.com/PDG/Tech-Sect-4.html, 1998.
Tournas VH, Katsoudas E. Mould and yeast flora in fresh berries, grapes and citrus fruits. Int. J. Food Microbiol., 2005; 105: 11-17.
Notermans S, Gallhoff G, Zweitering M, Mead G. Identification of critical control points in the HACCP system with a quantitative effect on the safety of food products. Food Microbiol., 12: 93-98, 1995.
Al-Hindawi N, Rished R. Presence and distribution of Salmonella species in some local foods from Baghdad City, Iraq. J. Food Protect., 1979; 42: 877-880.
Ercolani GL. Bacteriological quality assesment of fresh marketed lettuce and fennel. Appl. Environ. Microb., 1976; 31: 847-852.
Garcia-Villanova Ruiz B, Galvez Vargas R, Garcia-Villanova R. Contamination on fresh vegetables during cultivation and marketing. Int. J. Food Microbiol., 1987a; 4: 285-291.
Garcia-Villanova Ruiz B, Cuetoespinaz A, Bolonos MJ. A comparative study of Salmonella isolated from irrigation waters, vegetables and human infections. Epidemiol. Infect., 1987b; 98: 271-276.
Jerngklinchan J, Saitanu K. The occurrence of salmonellae in bean sprouts in Thailand. Southeast Asian J. Trop. Med. Public Health, 1993; 25: 114-118.
Arumugaswamy RKG, Rahamat RAG, Nadzriah BAHS. Prevalance of Listeria monocytogenes in foods in Malaysia. Int. J. Food Microbiol., 1994; 23: 117-121.
Breer C, Baumgartner A. Occurence and behavior of Listeria monocytogenes in salads, vegetables, and fresh vegetables juice. Arch. Lebensmittelhyg. 1992; 43: 108-110.
De Simon M, Tarrago C, Ferrer MD. Incidence of Listeria monocytogenes in fresh foods in Barcelona (Spain). Int. J. Food Microbiol., 1992; 16: 153-156.
Harvey J, Gilmour A. Occurence and characteristics of Listeria in foods produced in Nothern Ireland. Int. J. Food Microbiol., 1993; 19: 193-205.
Heisick JE, Wagner DE, Nierman ML, Peeler JT. Listeria spp. found on fresh market produce. Appl. Environ. Microb., 1989; 55: 1925-1927.
MacGowan AP, Bowker K, McLauchlin J, Bennet PM, Reeves DS. The occurence and seasonal changes in the isolation of Listeria spp. in shop bought food stuffs, human faeces, sewage and soil from urban sources. Int. J. Food Microbiol., 1994; 21: 325-334.
Wong HC, Chao WL, Lee SJ. Incidence and characterization of Listeria monocytogenes in foods available in Taiwan. Appl. Environ. Microb., 1990; 56: 3101-3104.
Chai LC, Robin T, Ragavan UM, Gunsalam JW, Bakar FA, Ghazali FM, Radu S, Kumar MP. Thermophilic Campylobacter spp. in salad vegetables in Malaysia. Int. J. Food Microbiol., 2007; 117: 106-111.
Park CE, Sanders GW. Occurence of thermotolerant campylobacters in fresh vegetables sold at farmers outdoor markets and supermarkets. Can. J. Microbiol., 1991; 38: 313-316.
Whyte P, McGill K, Cowley D, Madden RH, Moran L, Scates P, Carrol C, O’Leary A, Fanning S, Collins JD, McNamara E, Moore JE, Cormican M. Occurence Campylobacter in retail foods in Ireland. Int. J. Food Micr obiol., 2004;95: 111-118.
Erol İ. Gıda kaynaklı patojen bakteriler. In: Gıda hijyeni ve mikrobiyolojisi. Pozitif Matbaacılık Ltd., Ankara, 2007, pp. 71-77.