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Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant

Received: 5 October 2014    Accepted: 25 October 2014    Published: 29 January 2015
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Abstract

Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 101 to 106cfu/cm2. The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (106cfu/cm2) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.

Published in Journal of Food and Nutrition Sciences (Volume 3, Issue 1-2)

This article belongs to the Special Issue Food Processing and Food Quality

DOI 10.11648/j.jfns.s.2015030102.15
Page(s) 28-32
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Biofilm Formation, Microflora, Stainless Steel Coupon, Seafood Processing

References
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Cite This Article
  • APA Style

    Bui Thi Quynh Hoa, Warapa Mahakarnchanakul, Tanaboon Sajjaanantakul, Vichien Kitpreechavanich. (2015). Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant. Journal of Food and Nutrition Sciences, 3(1-2), 28-32. https://doi.org/10.11648/j.jfns.s.2015030102.15

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    ACS Style

    Bui Thi Quynh Hoa; Warapa Mahakarnchanakul; Tanaboon Sajjaanantakul; Vichien Kitpreechavanich. Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant. J. Food Nutr. Sci. 2015, 3(1-2), 28-32. doi: 10.11648/j.jfns.s.2015030102.15

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    AMA Style

    Bui Thi Quynh Hoa, Warapa Mahakarnchanakul, Tanaboon Sajjaanantakul, Vichien Kitpreechavanich. Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant. J Food Nutr Sci. 2015;3(1-2):28-32. doi: 10.11648/j.jfns.s.2015030102.15

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  • @article{10.11648/j.jfns.s.2015030102.15,
      author = {Bui Thi Quynh Hoa and Warapa Mahakarnchanakul and Tanaboon Sajjaanantakul and Vichien Kitpreechavanich},
      title = {Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant},
      journal = {Journal of Food and Nutrition Sciences},
      volume = {3},
      number = {1-2},
      pages = {28-32},
      doi = {10.11648/j.jfns.s.2015030102.15},
      url = {https://doi.org/10.11648/j.jfns.s.2015030102.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jfns.s.2015030102.15},
      abstract = {Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 101 to 106cfu/cm2. The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (106cfu/cm2) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant
    AU  - Bui Thi Quynh Hoa
    AU  - Warapa Mahakarnchanakul
    AU  - Tanaboon Sajjaanantakul
    AU  - Vichien Kitpreechavanich
    Y1  - 2015/01/29
    PY  - 2015
    N1  - https://doi.org/10.11648/j.jfns.s.2015030102.15
    DO  - 10.11648/j.jfns.s.2015030102.15
    T2  - Journal of Food and Nutrition Sciences
    JF  - Journal of Food and Nutrition Sciences
    JO  - Journal of Food and Nutrition Sciences
    SP  - 28
    EP  - 32
    PB  - Science Publishing Group
    SN  - 2330-7293
    UR  - https://doi.org/10.11648/j.jfns.s.2015030102.15
    AB  - Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 101 to 106cfu/cm2. The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (106cfu/cm2) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.
    VL  - 3
    IS  - 1-2
    ER  - 

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Author Information
  • Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand

  • Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand

  • Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand

  • Department of Microbiology, Faculty of Science, Kasesart University, Bangkok 10900, Thailand

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