Study on the Effect of Several Natural Products on Tyrosine Damage Induced by Peroxynitrite
Journal of Food and Nutrition Sciences
Volume 5, Issue 5, September 2017, Pages: 184-191
Received: Aug. 7, 2017; Published: Aug. 7, 2017
Views 1979      Downloads 118
Xiaoyu Tang, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
Anqi Wei, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
Yan Wang, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
Yunjing Luo, College of Life Science and Bioengineering, Beijing University of Technology, Beijing, China
Article Tools
Follow on us
Peroxynitrite (ONOOˉ), a powerful oxidant, is produced by nitric oxide (NO.) and superoxide anion (O2.ˉ). Under the physiological condition, the ONOOˉ could oxidize the lipids, nitrifyproteins, damage DNA and others biomolecules, thereby harm human health. The study used natural products Capsanthin, Myricetin and Capsaicin as the research object and controlled with Vc, and developed the method of HPLC-DAD to separate the components of nitrification damage system, which could determine the inhibition rate of natural products on the formation of 3-nitrotyrosine (3-NT). The fluorescence spectrometry was employed to determine the ability of these substances to inhibit tyrosine dimer and inhibit the self-oxidation of phthalate. The results showed that Capsanthin, Myricetin and Capsaicin had strong inhibitory effect on the generation capacity of 3-NT and tyrosine dimer, and strong inhibitory effect on the self-oxidation of phthalate.
HPLC-UV, Fluorescence Spectrometry, Peroxynitrite, Tyrosine Damage, Flavonoid Pigments, Pyrogallol
To cite this article
Xiaoyu Tang, Anqi Wei, Yan Wang, Yunjing Luo, Study on the Effect of Several Natural Products on Tyrosine Damage Induced by Peroxynitrite, Journal of Food and Nutrition Sciences. Vol. 5, No. 5, 2017, pp. 184-191. doi: 10.11648/j.jfns.20170505.14
F Gerardo, R Rafael. Chemical biology of peroxynitrite: kinetics, diffusion, and radicals [J]. Acs Chemical Biology. 2009, 4 (3): 161-177.
J Novak, J Suttnar, L Chrastinova, et al. 3-Nitrotyrosine in Sera of Patients with Myelodysplastic Syndromes, a Preliminary Study [J]. Blood. 2014, 124 (21): 5624.
K Chandrasekaran, K Swaminathan, S Chatterjee, et al. Apoptosis in HepG2 cells exposed to high glucose [J]. Toxicology in Vitro. 2010, 24 (2): 387-396.
S Pennathur, V Jackson-Lewis, S Przedborski, et al. Mass Spectrometric Quantification of 3-Nitrotyrosine, ortho-Tyrosine, and Dityrosine in Brain Tissue of 1-Methyl-4-phenyl-1, 2, 3, 6- tetrahydropyridine-treated Mice, a Model of Oxidative Stress in Parkinson's Disease [J]. The Journal of Biological Chemistry, 1999, 274 (1): 34621-34628.
R. Radi, Peroxynitrite, a stealthy biological oxidant, J. Biol. Chem. 2013 (288) 26464-26472.
C. Szabo, H. Ischiropoulos, R. Radi. Peroxynitrite: bioche- mistry, pathophysiologyand development of therapeutics, Nat. Rev. Drug Discov. 2007 (6) 662-680.
X Li. Anti-free radical action of tea polyphenols [J]. Science and technology horizon, 2014 (25): 352-353.
J M. Dimitric, P Boris, D Milenkovic, et al. Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms [J]. Food Chemistry, 2017 (218): 440-446.
W E Zhan, J Y Huang, W F Wang et al. Reaction of carotenoids and nitrogen dioxide free radical (NO2) [J]. Journal of chemistry of higher schools. 2006, 27 (3): 556-558.
J Zhang, X Hou, H Ahmad, et al. Assessment of free radicals scavenging activity of seven natural pigments and protective effects in AAPH-challenged chicken erythrocytes [J]. Food Chemistry, 2014 (145): 57-65.
X Q Tian, K Tian, C Y Chen. Research progress on antioxidant activity of curcumin and its structure-effect relationship [J]. Journal of yunnan institute of Chinese medicine, 2013 (01): 94-97.
X Zhang, X Z Zhao. Antioxidant Activities of Some Polyphenols Evaluated by Different Chemical Methods and Correlation Analysis [J]. Food science, 2008, 10: 85-89.
Q Feng, Y Torii, K Uchida, et al. Black tea polyphenols, theaflavins, prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P 450 1 A1 in cell cultures [J]. Journal of Agricultural Food Chemistry, 2002, 50 (1): 213-220.
H Chang, M T Mi, Y YGu, et al. Effects of flavonoids with different structures on proliferation of leukemia cell Line HL-60 [J]. Cancer. 2007 (12): 1309-1314.
Y Z Li, W Chen. Inhibitory effect of genistein on cervical carcinoma cell through AMPK and mTOR signaling pathway
[J]. Chinese clinical pharmacology and therapeutics. 2014 (01): 15-22.
H W Jie, Z Jing, G Ying, et al. Study on the hypoglycemi- ceffectof isoamyl alkenyl flavonoids of Licorice [J]. Northern pharmacology. 2014 (10): 65-68.
M Wang, Z G Sun, W Q Liu, et al. Effects of Soy Isoflavones on Concentrations of Interferon γ, Interleukin 2 and Interleukin 4 and mRNA Expression of Estrogen Receptor β in Lymphocytes of Spleen and Intestinal Lymph Nodes of Dairy Cows [J]. Journal of animal nutrition. 2012 (5): 859-869.
Y Q Zhang, S Wang, J Jiao, et al. Anti-virus function of 5 kinds of total flavone ingredients in vitro [J]. Journal of nanjing agricultural university. 2012 (04): 105-109.
KUlanowska, AMajchrzyk, Marta Moskot, et al. Assessment of antibacterial effects of flavonoids by estimation of generation times in liquid bacterial cultures [J]. Biologia, 2007, 62 (2): 132-135.
Y Jian, H J Zheng, J JJin, et al. Fluorescence spectroscopy study on the interaction between Gossypol and bovine serum albumin [J]. Molecular Structure. 2009 (920): 227-230.
G Romain, P JFrançois, Myricetin, rosmarinic and carnosic acids as superior natural antioxidantalternatives toα-tocopherol for the preservation of omega-3 oils [J]. Food Chemistry, 2016 (213) 284–295.
G C Vazhappilly, D Graham, Plant flavonoids in cancer chemoprevention: role in genome stability [J]. The Journal of Nutritional Biochemistry, 2007 (45) 1-14.
Z Lou, Q Zhao, J X Liu, et al. Fluorescent real-time quantitative measurements of intracellularperoxy nitrite generation and inhibition [J]. Analytical Biochemistry 2017 (520) 44-48.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186