The Dietary Combination of Quercetin and Resveratrol Supplementation May Improve Exercise Tolerance in Young Untrained Males by Modulating IL-6 and NGAL Response
American Journal of Sports Science
Volume 3, Issue 2, March 2015, Pages: 29-35
Received: Feb. 15, 2015; Accepted: Feb. 27, 2015; Published: Mar. 4, 2015
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Zekine Punduk, University of Balikesir, Physical Education and Sports, Balikesir, Turkey
Adnan Adil Hismiogullari, University of Balikesir, Faculty of Medicine, Department of Medical Biochemistry, Balikesir Turkey
Ozlem Yavuz, University of Balikesir, Faculty of Medicine, Department of Medical Biochemistry, Balikesir Turkey
Khalid Rahman, Liverpool John Moores University, Faculty of Science, Schoool of Pharmacy and Biomolecular Sciences, Liverpool, England (UK)
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The effect of antioxidant supplementation on exercise performance has not been clearly defined in trained or un-trained individuals. The purpose of this investigation was to test the effects of quercetin-resveratrol combination on plasma anti-inflammatory response and exercise performance in untrained men. After baseline cycling performance testing, the subjects (n=8) were supplemented daily with 500gr quercetin and 500 gr resveratrol for 21 days. The complete blood counts, creatinine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukin-6 (IL-6), Neutrophil gelatinase-associated lipocalin (NGAL) and exercise performance parameters were evaluated at baseline, after 21 days of supplementation and 15 days after the end of the study. The maximal aerobic power (POmax) was increased (19%) in the post-supplementation period compare to baseline and washout period. In the post-supplementation period, the time trial score (TTscore), maximal and average speed significantly increased in the 10 km time trial cycling test compare to the baseline level. Importantly, in response to supplementation, the serum IL-6 and N-GAL levels were upregulated post-supplementation and these levels returned to baseline levels at the end of the washout period. These results suggest that dieatary combination of quercetin and resveretrol may act as a potential tool for enhancing exercise performance by regulation IL-6, NGAL level in untrained men.
NGAL, IL6, Cycling performance, Quercetin, Resveratrol
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Zekine Punduk, Adnan Adil Hismiogullari, Ozlem Yavuz, Khalid Rahman, The Dietary Combination of Quercetin and Resveratrol Supplementation May Improve Exercise Tolerance in Young Untrained Males by Modulating IL-6 and NGAL Response, American Journal of Sports Science. Vol. 3, No. 2, 2015, pp. 29-35. doi: 10.11648/j.ajss.20150302.11
Harwood M, Danielewska-Nikiel B, Borzelleca JF, Flamm GW, Williams GM, Lines TC. A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties.Food ChemToxicol. 2007; 45: 2179–2205.
Voduc N, la Porte C, Tessier C, Mallick R, Cameron DW .Effect of resveratrol on exercise capacity: a randomized placebo-controlled crossover pilot study. ApplPhysiolNutrMetab. 2014; 39: 1-5.
Vang O, Ahmad N, Baile CA, Baur JA, Brown K, Csiszar A, Das DK, Delmas D, Gottfried C, Lin HY, Ma QY, Mukhopadhyay P, Nalini N, Pezzuto JM, Richard T,Shukla Y, Surh YJ, Szekeres T, Szkudelski T, Walle T, Wu JM.What is new for an old molecule? Systematic review and recommendations on the use of resveratrol.Plos one 2011. 6: e19881. doi:10.1371/journal.pone. 0019881. PMID: 21698226.
Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Mortality in randomized trials of antioxidant supplements for primary and secondary prevention: Systematic review and meta-analysis.Jama. 2007; 297: 842–857.
Davis JM, Murphy EA, Carmichael MD.Effects of the dietary flavonoid quercetin upon performance and health. Curr Sports Med Rep. 2009a; 8: 206–213.
Davis JM, Murphy EA, Carmichael MD, Davis B.Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance.Am J PhysiolRegulIntegr CompPhysiol.2009b; 296: 1071–1077.
Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, Geny B, Laakso M, Puigserver P, Auwerx J.Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1.Cell. 2006; 127: 1109–1122.
Murase T, Haramizu S, Ota N, and Hase T.Suppression of the aging associated decline in physical performance by a combination of resveratrol intake and habitual exercise insenescence-accelerated mice.Biogerontology.2009;10: 423–434.
Dolinsky VW, Jones KE, Sidhu RS, Haykowsky M,Czubryt MP, Gordon T, Dyck JRB.Improvements in skeletal muscle strength and cardiac function induced by resveratrol during exercise training contribute to enhanced exercise performance in rats.J Physiol. 2012;590: 2783–2799.
Hart N, Sarga L, Csende Z, Koltai E, Koch LG, Britton SL, Davies KJ, Kouretas D, Wessner B, Radak Z.Resveratrol enhances exercise training responses in rats selectively bred for high running performance. Food ChemToxicol. 2013;61: 53–59.
Wu RE, Huang WC, Liao CC, Chang YK, Kan NW, Huang CC: Resveratrol protects against physical fatigue and improves exercise performance in mice. Molecules. 2013;18:4689–4702.
Malaguti M, Angeloni C, Hrelia S.Polyphenols in exercise performance and prevention of exercise-induced muscle damage.Oxid Med Cell Longe.2013, 825928. doi: 10. 1155 /2013/825928. Epub 2013 Jul 24.
Nieman DC.Immunonutrition support for athletes.Nutr Rev.2008;66:310–320.
Nieman DC, Henson DA, Maxwell KR, Williams AS, McAnulty SR, Jin F, Shanely RA, Lines TC.Effects of quercetin and EGCG on mitochondrial biogenesis and immunity.Med Sci Sports Exerc. 2009;41:1467–1475.
Cureton KJ, Tomporowski PD, Singhal A,Pasley JD, Bigelman KA, Lambourne K, Trilk JL, McCully KK, Arnaud MJ, Zhao Q.Dietary quercetin supplementation is not ergogenic in untrained men.J ApplPhysio.2009;107:1095–1104.
NiemanDC, Williams AS, Shanely RA, Jin F, McAnuty SR, Triplett NT, Austin MD, Henson DA. Quercetin´s influence on exercise performance and muscle mitochondrial biogenesis. Med Sci Sports Exerc. 2010;42:338–345.
Davis JM, Carlstedt CJ, Chen S, Carmichael MD, Murphy EA.The dietary flavonoid quercetin increases VO2max and endurance capacity. Int J Sport NutrExerc Meta. 2010; 20:56–62.
Quindry JC, McAnulty SR, Hudson MB, Hosick P, Dumke C, McAnulty LS, Henson D, Morrow JD, Nieman D.Oral quercetin supplementation and blood oxidative capacity in response to ultramarathon competition. Int J Sport NutrExerc Meta. 2008; 18:601–616.
MacRae HS, Mefferd KM.Dietary antioxidant supplementation combined with quercetin improves cycling time trial performance. Int J Sport NutrExercMetab. 2006; 16:405–419
Bigelman KA, Fan EH, Chapman DP, Freese EC, Trilk JL, Curetok KJ.Effects of six weeks of quercetin supplementation on physical performance in ROTC cadets. Mil Med. 2010;175:791-798.
GabayC. Interleukin-6 and chronic inflammation. Arthritis Res Ther. 2006; 8:2–3.
Villarino A, Huang E, Hunter CA.Understanding the pro-and anti inflammatory properties of IL-27. J Immunol.2004 ; 173:715–720.
Heinrich PC, Castell JV, Andus T.Interleukin-6 and the acute phase response.Biochem J. 265:621–636.
Van Snick J.Interleukin-6: an overview. Annu Rev Immunol. 1990; 8: 253–278.
Dann SM, Spehlmann ME, Hammond DC, Iimura M,Hase K, Choi LJ, Eckmann L.IL-6-dependent mucosal protection prevents establishment of a microbial niche for attaching/effacing lesion-forming enteric bacterial pathogens.J Immunol. 2008; 180: 6816-6826.
Scheller J, Chalaris A, Schmidt-Arras D, Rose-John S.The pro- and anti-inflammatory properties of the cytokine interleukin-6.BiochimBiophysActa. 2011; 1813:878-888.
Kelly M, Gauthier MS, Saha AK, Ruderman NB.Activation of AMP-activated protein kinase by interleukin-6 in rat skeletal muscle: Association with changes in cAMP, energy state, and endogenous fuel mobilization.Diabetes.2009;58:1953-1960.
Febbraio MA, SteensbergA, Keller C, Starkie RL, Nielsen HB, Krustrup P, Pedersen BK.Glucose ingestion attenuates interleukin-6 release from contracting skeletal muscle in humans. J Physiol. 2003;549: 607-612.
Starkie RL, Arkinstall MJ, Koukoulas I, Hawley JA, Febbraio MA.Carbohydrate ingestion attenuates the increase in plasma interleukin-6, but not skeletal muscle interleukin-6 mRNA, during exercise in humans. J Physiol. 2001; 533: 585-591
Triebel S, Bläser J, Reinke H, Tschesche H.A 25 kDa 2-microglobulin-related protein is a component of the 125 kDa form of human gelatinase.FEBS Lett. 1992; 314:386-388.
Cowland JB,Borregaard N.Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans.Genomics. 1997;45:17-23.
LiuQ, Nilsen HM.Identification of a new acute phase protein.J Biol Chem.1995;270: 22565–22570.
Halawa A.The early diagnosis of acute renal graft dysfunction: A challenge we face. The role of novel biomarkers.Ann Transplant. 2011;16: 90-98.
Roudkenar MHR, Halabian Z, Ghasemipour AM, Roushandeh M, Rouhbakhsh M, Nekogoftar Y, Kuwahara M, Fukumoto M, Shokrgozar MA.Neutrophil gelatinase-associated lipocalin acts as a protective factor against H(2)O(2)toxicity.Arch Med Res. 2008;39:560-566.
Roudkenar MH, Kuwahara Y, Baba T. Roushandeh AM, Ebishima S, Abe S, Ohkubo Y, Fukumoto M.Oxidative stress induced lipocalin2 gene expression: addressing its expression under the harmful conditions. J Radiat Res. 2007; 48:39-44.
Hettinga FJ, De Koning JJ, Broersen FT, Vangeffen P, Foster C: Pacing strategy and the occurrence of fatigue in 4000-m cycling time trials.Med Sci Sports Exerc. 2006; 38:1484–1491.
Hawley JA, Noakes TD.Peak power output predicts maximal oxygen uptake and performance time in trained cyclists. Eur J Appl Physiol. 1992;65:79-83.
Ganio MS, Armstrong LE, Johnson EC, Klau JF, Ballard KD, Michniak-Kohn B, Kaushik D, Maresh CM. Effectofquercetin supplementation on maximal oxygen uptake in men and women.J Sports Sci. 2010; 28:201–208.
Utter AC, Nieman DC, Kang J, Dumke CL, Quindry JC, McAnulty SR, McAnulty LS.Quercetin does not affect rating of perceived exertion in athletes during the western states endurance run,Res Sports Med. 2009; 17:71–83.
Scholten SD, Sergeev IN.Long term quercetin supplementation reduces lipid peroxidation but does not improve performance in endurance runners. Open Access J Sports Med. 2013; 12:4:53-61.
Casuso RA, Martı´nez-Amat A, Martı´nez-Lo´pez EJ, Camiletti-Moiro´nD, Porres JM, Aranda P.Ergogenic effects of quercetin supplementation in trained rats.J IntSoc Sports Nutr. 2013a;10:3.doi: 10.1186/1550-2783-10-3.
Casuso RA, Lopez EJM, Contreras FH, Moiron DC, Romero RM, Canuelo A, Amat AM.The combination of oral quercetin supplementation and exercise prevents brain mitochondrial biogenesis.Genes Nutr. 2014;9:420-428.
Kuennen M, Gillum T, Dokladny K, Bederick E, Schenider S, Moseley P.Thermotolerance and heat acclimation may share a common mechanism in humans.Am J PhysiolRegulIntegr Comp Physiol.2011;301:524–533.
Starkie R, Ostrowski SR, Jauffred S, FebbraioM, Pedersen BK. Exercise and IL-6 infusion inhibit endotoxin-induced TNF-alpha production in humans.FASEB J. 2003; 17: 884-886.
Kado S, Nagase T, Nagata N.Circulating levels of interleukin-6, its soluble receptor nd interleukin-6/ interleukin-6 receptor complexes in patients with type 2 diabetes mellitus.ActaDiabetol. 1999; 36: 67–72.
Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK.Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans.J Physiol. 1999;515:287–291.
Junglee NA, Di Felice U, Dolci A, Fortes MB, Jibani MM, Lemmey AB, Walsh NP, Macdonald JH. Exercising in a hot environment with muscle damage:effects on acute kidney injury biomarkers and kidney function. Am J Physiol Renal Physiol. 2013;305:813-820.
Kanda K, Sugama K, Sakuma J, Kawakami Y, Suzuki K.Evaluation of serum leaking enzymes and investigation into new biomarkers for exercise-induced muscle damage. ExercImmunol Rev. 2014;20:39-54.
Helge JW, Klein DK, Andersen TM, van, HG, Calbet JL, Boushel RC, SaltinB.IL-6 release is higher across arm than leg muscles during whole body exercise.ExpPhysiol 2011.96;590-598.
Sarvas JL, Khaper N, Lees SJ. 2013.The IL-6 paradox: Context dependent interplay of SOCS3 and AMPK.J Diabetes Metab. 2013; doi:10.4172/2155-6156.S13-003.
Kahn BB, Alquier T, Carling D, Hardie DG.AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab 2005;1: 15-25.
Martin TL, Alquier T, Asakura K, Furukawa N, Preitner F, Kahn BB.Diet-induced obesity alters AMP kinase activity in hypothalamus and skeletal muscle. J Biol Chem. 2006;281:18933–18941.
Roudkenar MH, Halabian R, Roushandeh AM, Nourani MR, MasrooriN, Ebrahimi M, Nikogoftar M, Rouhbakhsh M, Bahmani P,Najafabadi AJ, Shokrgozar MA.Lipocalin 2 regulation by thermal stresses: protective role of Lcn2/NGAL against cold and heat stresses.Exp Cell Res. 2009, 315: 3140–3151.
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