Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans

Anastassios Philippou; Maria Maridaki; Constantinos Psarros; Michael Koutsilieris

doi:doi:10.11648/j.ajss.20180602.11

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Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans

Anastassios Philippou, Maria Maridaki, Constantinos Psarros, Michael Koutsilieris

Published in American Journal of Sports Science (Volume 6, Issue 2)

Received: 11 October 2017 Accepted: 26 October 2017 Published: 16 March 2018

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Abstract

Background: Exercise-induced muscle damage is followed by muscle adaptation which has been associated with an inflammatory response and is influenced by a crucial balance between pro- and anti-inflammatory cytokines. This study investigated the pattern of systemic cytokine responses for several hours after muscle-damaging exercise. Methods: Nine healthy, young men volunteers performed 50 maximal eccentric muscle actions with each leg using the knee extensors. Serum levels of interleukin (IL)-1α, IL-2, IL-6 and IL-10, transforming growth factor (TGF)-β1 and tumor necrosis factor (TNF)-α were measured by ELISA before and at 6, 48 and 120 hours post-exercise. Results: Volunteers reported significant muscle soreness and their serum creatine kinase (CK) activity increased gradually up to 120 hrs post-exercise (p<0.05). Circulating levels of IL-1a remained unaltered and TGF-β1 increased slightly over time, while IL-2 showed a moderate increase 48 hrs following eccentric exercise (p>0.05). Levels of TNF-α and IL-10 exhibited a similar pattern of response over time, showing a nearly 50% and 100% increase, respectively, 6 hrs post-exercise, while IL-6 increase significantly 6 and 48 hrs post-exercise (p<0.05). Conclusion: These findings suggest that eccentric exercise might trigger a systemic, predominantly anti-inflammatory, acute cytokine response as part of the adaptation process to muscle damage, where IL-6 may be especially involved.

Published in	American Journal of Sports Science (Volume 6, Issue 2)
DOI	10.11648/j.ajss.20180602.11
Page(s)	32-37
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Cytokines, IL-6, IL-10, Muscle Damage, TGF-β1, TNF-α

References

[1]	Peake JM, Neubauer O, Della Gatta PA, Nosaka K, Muscle damage and inflammation during recovery from exercise. J Appl Physiol (1985) 2016; 122: 559-570.
[2]	Philippou A, Bogdanis GC, Nevill AM, Maridaki M, Changes in the angle-force curve of human elbow flexors following eccentric and isometric exercise. Eur J Appl Physiol 2004; 93: 237-244.
[3]	Philippou A, Maridaki M, Bogdanis G, Halapas A, Koutsilieris M, Changes in the mechanical properties of human quadriceps muscle after eccentric exercise. In Vivo 2009; 23: 859-865.
[4]	Philippou A, Maridaki M, Bogdanis GC, Angle-specific impairment of elbow flexors strength after isometric exercise at long muscle length. J Sports Sci 2003; 21: 859-865.
[5]	Kanda K, Sugama K, Hayashida H, Sakuma J, Kawakami Y, Miura S, Yoshioka H, Mori Y, Suzuki K, Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation. Exerc Immunol Rev 2013; 19: 72-85.
[6]	Philippou A, Maridaki M, Theos A, Koutsilieris M, Cytokines in muscle damage. Adv Clin Chem 2012; 58: 49-87.
[7]	Smith LL, Anwar A, Fragen M, Rananto C, Johnson R, Holbert D, Cytokines and cell adhesion molecules associated with high-intensity eccentric exercise. Eur J Appl Physiol 2000; 82: 61-67.
[8]	Peake J, Nosaka K, Suzuki K, Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev 2005; 11: 64-85.
[9]	Suzuki K, Nakaji S, Yamada M, Totsuka M, Sato K, Sugawara K, Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc Immunol Rev 2002; 8: 6-48.
[10]	Pedersen BK, Hoffman-Goetz L, Exercise and the immune system, Regulation, integration, and adaptation. Physiol Rev 2000; 80: 1055-1081.
[11]	Hirose L, Nosaka K, Newton M, Laveder A, Kano M, Peake J, Suzuki K, Changes in inflammatory mediators following eccentric exercise of the elbow flexors. Exerc Immunol Rev 2004; 10: 75-90.
[12]	Smith C, Kruger MJ, Smith RM, Myburgh KH, The inflammatory response to skeletal muscle injury: Illuminating complexities. Sports Med 2008; 38: 947-969.
[13]	Figarella-Branger D, Civatte M, Bartoli C, Pellissier JF, Cytokines, chemokines, and cell adhesion molecules in inflammatory myopathies. Muscle Nerve 2003; 28: 659-682.
[14]	Tidball JG, Villalta SA, Regulatory interactions between muscle and the immune system during muscle regeneration. Am J Physiol Regul Integr Comp Physiol 2010; 298: R1173-1187.
[15]	Karalaki M, Fili S, Philippou A, Koutsilieris M, Muscle regeneration: Cellular and molecular events. In Vivo 2009; 23: 779-796.
[16]	van de Vyver M, Myburgh KH, Cytokine and satellite cell responses to muscle damage: Interpretation and possible confounding factors in human studies. J Muscle Res Cell Motil 2012; 33: 177-185.
[17]	Pedersen BK, Akerstrom TC, Nielsen AR, Fischer CP, Role of myokines in exercise and metabolism. J Appl Physiol (1985) 2007; 103: 1093-1098.
[18]	Frost RA, Lang CH, Skeletal muscle cytokines: Regulation by pathogen-associated molecules and catabolic hormones. Curr Opin Clin Nutr Metab Care 2005; 8: 255-263.
[19]	Strassmann G, Patil-Koota V, Finkelman F, Fong M, Kambayashi T, Evidence for the involvement of interleukin 10 in the differential deactivation of murine peritoneal macrophages by prostaglandin e2. J Exp Med 1994; 180: 2365-2370.
[20]	Arnold L, Henry A, Poron F, Baba-Amer Y, van Rooijen N, Plonquet A, Gherardi RK, Chazaud B, Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis. J Exp Med 2007; 204: 1057-1069.
[21]	Villalta SA, Nguyen HX, Deng B, Gotoh T, Tidball JG, Shifts in macrophage phenotypes and macrophage competition for arginine metabolism affect the severity of muscle pathology in muscular dystrophy. Hum Mol Genet 2009; 18: 482-496.
[22]	Petersen AM, Pedersen BK, The anti-inflammatory effect of exercise. J Appl Physiol (1985) 2005; 98: 1154-1162.
[23]	Reihmane D, Dela F, Interleukin-6: Possible biological roles during exercise. Eur J Sport Sci 2014; 14: 242-250.
[24]	Steensberg A, Fischer CP, Keller C, Moller K, Pedersen BK, Il-6 enhances plasma il-1ra, il-10, and cortisol in humans. Am J Physiol Endocrinol Metab 2003; 285:E433-437.
[25]	Philippou A, Bogdanis G, Maridaki M, Halapas A, Sourla A, Koutsilieris M, Systemic cytokine response following exercise-induced muscle damage in humans. Clin Chem Lab Med 2009; 47: 777-782.
[26]	Zembron-Lacny A, Naczk M, Gajewski M, Ostapiuk-Karolczuk J, Dziewiecka H, Kasperska A, Szyszka K, Changes of muscle-derived cytokines in relation to thiol redox status and reactive oxygen and nitrogen species. Physiol Res 2010; 59: 945-951.
[27]	Bouche M, Canipari R, Melchionna R, Willems D, Senni MI, Molinaro M, Tgf-beta autocrine loop regulates cell growth and myogenic differentiation in human rhabdomyosarcoma cells. FASEB J 2000; 14: 1147-1158.
[28]	Philippou A, Maridaki M, Koutsilieris M, The role of urokinase-type plasminogen activator (upa) and transforming growth factor beta 1 (tgfbeta1) in muscle regeneration. In Vivo 2008; 22: 735-750.
[29]	Han G, Li F, Singh TP, Wolf P, Wang XJ, The pro-inflammatory role of tgfbeta1: A paradox? Int J Biol Sci 2012; 8: 228-235.
[30]	Gao HG, Fisher PW, Lambi AG, Wade CK, Barr-Gillespie AE, Popoff SN, Barbe MF, Increased serum and musculotendinous fibrogenic proteins following persistent low-grade inflammation in a rat model of long-term upper extremity overuse. PLoS One 2013; 8: e71875.

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APA Style

Anastassios Philippou, Maria Maridaki, Constantinos Psarros, Michael Koutsilieris. (2018). Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. American Journal of Sports Science, 6(2), 32-37. https://doi.org/10.11648/j.ajss.20180602.11

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ACS Style

Anastassios Philippou; Maria Maridaki; Constantinos Psarros; Michael Koutsilieris. Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. Am. J. Sports Sci. 2018, 6(2), 32-37. doi: 10.11648/j.ajss.20180602.11

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AMA Style

Anastassios Philippou, Maria Maridaki, Constantinos Psarros, Michael Koutsilieris. Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. Am J Sports Sci. 2018;6(2):32-37. doi: 10.11648/j.ajss.20180602.11

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@article{10.11648/j.ajss.20180602.11,
  author = {Anastassios Philippou and Maria Maridaki and Constantinos Psarros and Michael Koutsilieris},
  title = {Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans},
  journal = {American Journal of Sports Science},
  volume = {6},
  number = {2},
  pages = {32-37},
  doi = {10.11648/j.ajss.20180602.11},
  url = {https://doi.org/10.11648/j.ajss.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajss.20180602.11},
  abstract = {Background: Exercise-induced muscle damage is followed by muscle adaptation which has been associated with an inflammatory response and is influenced by a crucial balance between pro- and anti-inflammatory cytokines. This study investigated the pattern of systemic cytokine responses for several hours after muscle-damaging exercise. Methods: Nine healthy, young men volunteers performed 50 maximal eccentric muscle actions with each leg using the knee extensors. Serum levels of interleukin (IL)-1α, IL-2, IL-6 and IL-10, transforming growth factor (TGF)-β1 and tumor necrosis factor (TNF)-α were measured by ELISA before and at 6, 48 and 120 hours post-exercise. Results: Volunteers reported significant muscle soreness and their serum creatine kinase (CK) activity increased gradually up to 120 hrs post-exercise (p0.05). Levels of TNF-α and IL-10 exhibited a similar pattern of response over time, showing a nearly 50% and 100% increase, respectively, 6 hrs post-exercise, while IL-6 increase significantly 6 and 48 hrs post-exercise (p<0.05). Conclusion: These findings suggest that eccentric exercise might trigger a systemic, predominantly anti-inflammatory, acute cytokine response as part of the adaptation process to muscle damage, where IL-6 may be especially involved.},
 year = {2018}
}

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TY  - JOUR
T1  - Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans
AU  - Anastassios Philippou
AU  - Maria Maridaki
AU  - Constantinos Psarros
AU  - Michael Koutsilieris
Y1  - 2018/03/16
PY  - 2018
N1  - https://doi.org/10.11648/j.ajss.20180602.11
DO  - 10.11648/j.ajss.20180602.11
T2  - American Journal of Sports Science
JF  - American Journal of Sports Science
JO  - American Journal of Sports Science
SP  - 32
EP  - 37
PB  - Science Publishing Group
SN  - 2330-8540
UR  - https://doi.org/10.11648/j.ajss.20180602.11
AB  - Background: Exercise-induced muscle damage is followed by muscle adaptation which has been associated with an inflammatory response and is influenced by a crucial balance between pro- and anti-inflammatory cytokines. This study investigated the pattern of systemic cytokine responses for several hours after muscle-damaging exercise. Methods: Nine healthy, young men volunteers performed 50 maximal eccentric muscle actions with each leg using the knee extensors. Serum levels of interleukin (IL)-1α, IL-2, IL-6 and IL-10, transforming growth factor (TGF)-β1 and tumor necrosis factor (TNF)-α were measured by ELISA before and at 6, 48 and 120 hours post-exercise. Results: Volunteers reported significant muscle soreness and their serum creatine kinase (CK) activity increased gradually up to 120 hrs post-exercise (p0.05). Levels of TNF-α and IL-10 exhibited a similar pattern of response over time, showing a nearly 50% and 100% increase, respectively, 6 hrs post-exercise, while IL-6 increase significantly 6 and 48 hrs post-exercise (p<0.05). Conclusion: These findings suggest that eccentric exercise might trigger a systemic, predominantly anti-inflammatory, acute cytokine response as part of the adaptation process to muscle damage, where IL-6 may be especially involved.
VL  - 6
IS  - 2
ER  -

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Author Information

Anastassios Philippou

Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
Maria Maridaki

Department of Sports Medicine & Biology of Physical Activity, School of Physical Education and Sport Science, National and Kapodistrian University of Athens, Athens, Greece
Constantinos Psarros

Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece
Michael Koutsilieris

Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece

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Plain Text BibTeX RIS

APA Style

Anastassios Philippou, Maria Maridaki, Constantinos Psarros, Michael Koutsilieris. (2018). Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. American Journal of Sports Science, 6(2), 32-37. https://doi.org/10.11648/j.ajss.20180602.11

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ACS Style

Anastassios Philippou; Maria Maridaki; Constantinos Psarros; Michael Koutsilieris. Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. Am. J. Sports Sci. 2018, 6(2), 32-37. doi: 10.11648/j.ajss.20180602.11

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AMA Style

Anastassios Philippou, Maria Maridaki, Constantinos Psarros, Michael Koutsilieris. Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans. Am J Sports Sci. 2018;6(2):32-37. doi: 10.11648/j.ajss.20180602.11

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@article{10.11648/j.ajss.20180602.11,
  author = {Anastassios Philippou and Maria Maridaki and Constantinos Psarros and Michael Koutsilieris},
  title = {Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans},
  journal = {American Journal of Sports Science},
  volume = {6},
  number = {2},
  pages = {32-37},
  doi = {10.11648/j.ajss.20180602.11},
  url = {https://doi.org/10.11648/j.ajss.20180602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajss.20180602.11},
  abstract = {Background: Exercise-induced muscle damage is followed by muscle adaptation which has been associated with an inflammatory response and is influenced by a crucial balance between pro- and anti-inflammatory cytokines. This study investigated the pattern of systemic cytokine responses for several hours after muscle-damaging exercise. Methods: Nine healthy, young men volunteers performed 50 maximal eccentric muscle actions with each leg using the knee extensors. Serum levels of interleukin (IL)-1α, IL-2, IL-6 and IL-10, transforming growth factor (TGF)-β1 and tumor necrosis factor (TNF)-α were measured by ELISA before and at 6, 48 and 120 hours post-exercise. Results: Volunteers reported significant muscle soreness and their serum creatine kinase (CK) activity increased gradually up to 120 hrs post-exercise (p0.05). Levels of TNF-α and IL-10 exhibited a similar pattern of response over time, showing a nearly 50% and 100% increase, respectively, 6 hrs post-exercise, while IL-6 increase significantly 6 and 48 hrs post-exercise (p<0.05). Conclusion: These findings suggest that eccentric exercise might trigger a systemic, predominantly anti-inflammatory, acute cytokine response as part of the adaptation process to muscle damage, where IL-6 may be especially involved.},
 year = {2018}
}

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TY  - JOUR
T1  - Systemic Responses of Inflammation-Related Factors Following Eccentric Exercise in Humans
AU  - Anastassios Philippou
AU  - Maria Maridaki
AU  - Constantinos Psarros
AU  - Michael Koutsilieris
Y1  - 2018/03/16
PY  - 2018
N1  - https://doi.org/10.11648/j.ajss.20180602.11
DO  - 10.11648/j.ajss.20180602.11
T2  - American Journal of Sports Science
JF  - American Journal of Sports Science
JO  - American Journal of Sports Science
SP  - 32
EP  - 37
PB  - Science Publishing Group
SN  - 2330-8540
UR  - https://doi.org/10.11648/j.ajss.20180602.11
AB  - Background: Exercise-induced muscle damage is followed by muscle adaptation which has been associated with an inflammatory response and is influenced by a crucial balance between pro- and anti-inflammatory cytokines. This study investigated the pattern of systemic cytokine responses for several hours after muscle-damaging exercise. Methods: Nine healthy, young men volunteers performed 50 maximal eccentric muscle actions with each leg using the knee extensors. Serum levels of interleukin (IL)-1α, IL-2, IL-6 and IL-10, transforming growth factor (TGF)-β1 and tumor necrosis factor (TNF)-α were measured by ELISA before and at 6, 48 and 120 hours post-exercise. Results: Volunteers reported significant muscle soreness and their serum creatine kinase (CK) activity increased gradually up to 120 hrs post-exercise (p0.05). Levels of TNF-α and IL-10 exhibited a similar pattern of response over time, showing a nearly 50% and 100% increase, respectively, 6 hrs post-exercise, while IL-6 increase significantly 6 and 48 hrs post-exercise (p<0.05). Conclusion: These findings suggest that eccentric exercise might trigger a systemic, predominantly anti-inflammatory, acute cytokine response as part of the adaptation process to muscle damage, where IL-6 may be especially involved.
VL  - 6
IS  - 2
ER  -

Copy | Download