Retaining ALK Rearrangement in Cultured Circulating Tumor Cells Derived from Lung Cancer Patients
Cancer Research Journal
Volume 3, Issue 1, January 2015, Pages: 11-16
Received: Jan. 26, 2015; Accepted: Feb. 14, 2015; Published: Feb. 25, 2015
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Authors
Eunjoo Hwang, Cytogen Inc., Seoul, Korea
Dong-Hyoung Lee, Cytogen Inc., Seoul, Korea
Ji-hyun Uh, Cytogen Inc., Seoul, Korea
Duyeol Han, Cytogen Inc., Seoul, Korea
Myoung Shin Kim, Cytogen Inc., Seoul, Korea
Sung Ho Choi, Cytogen Inc., Seoul, Korea
JooKyung Park, Department of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea
Byung Hee Jeon, Cytogen Inc., Seoul, Korea
Jinseon Lee, Cytogen Inc., Seoul, Korea
Se-Hoon Lee, Department of Internal Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea
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Abstract
Circulating tumor cells (CTCs) are rare cells that have shed into the bloodstream from primary tumor, and potentiallyprovidea tool for the better understanding of tumor metastasis and noninvasive monitoring of the disease progression. However their isolation and characterization has been a major technological challenge due to their rareness. Here, we suggest the CTC culture as an effective method to obtain CTCs sufficient in numberfor molecular analysis of original tumor characteristics. We isolated and successfully cultured the CTCs from four lung cancer patients, and then analyzed those cells for ALK (anaplastic lymphoma kinase) fusion using real-time PCR method, and confirmed that the cultured CTCs have retained thefusion the same as those found in primary tumors. These results suggest that the isolation and culture of CTCs can be a substitutive method for tumor tissue biopsy, and may provide practically useful clinical applications, such as personalized cancer therapy based on their genomic information through serial blood samplings from the cancer patients.
Keywords
Lung Cancer, Circulating Tumor Cells, ALKRearrangement, Real Time PCR
To cite this article
Eunjoo Hwang, Dong-Hyoung Lee, Ji-hyun Uh, Duyeol Han, Myoung Shin Kim, Sung Ho Choi, JooKyung Park, Byung Hee Jeon, Jinseon Lee, Se-Hoon Lee, Retaining ALK Rearrangement in Cultured Circulating Tumor Cells Derived from Lung Cancer Patients, Cancer Research Journal. Vol. 3, No. 1, 2015, pp. 11-16. doi: 10.11648/j.crj.20150301.13
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