Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells

Emmanuel Reyes-Uribe; Octavio Galindo-Hernandez; Pedro Cortes-Reynosa; Eduardo Perez Salazar

doi:doi:10.11648/j.crj.20150303.12

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Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells

Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar

Published in Cancer Research Journal (Volume 3, Issue 3)

Received: 6 May 2015 Accepted: 26 May 2015 Published: 16 June 2015

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Abstract

Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.

Published in	Cancer Research Journal (Volume 3, Issue 3)
DOI	10.11648/j.crj.20150303.12
Page(s)	52-62
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Collagen IV, DDR1, Akt2, PI3K, EGFR, Breast Cancer

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APA Style

Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar. (2015). Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Research Journal, 3(3), 52-62. https://doi.org/10.11648/j.crj.20150303.12

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ACS Style

Emmanuel Reyes-Uribe; Octavio Galindo-Hernandez; Pedro Cortes-Reynosa; Eduardo Perez Salazar. Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Res. J. 2015, 3(3), 52-62. doi: 10.11648/j.crj.20150303.12

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AMA Style

Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar. Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Res J. 2015;3(3):52-62. doi: 10.11648/j.crj.20150303.12

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@article{10.11648/j.crj.20150303.12,
  author = {Emmanuel Reyes-Uribe and Octavio Galindo-Hernandez and Pedro Cortes-Reynosa and Eduardo Perez Salazar},
  title = {Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells},
  journal = {Cancer Research Journal},
  volume = {3},
  number = {3},
  pages = {52-62},
  doi = {10.11648/j.crj.20150303.12},
  url = {https://doi.org/10.11648/j.crj.20150303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20150303.12},
  abstract = {Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.},
 year = {2015}
}

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TY  - JOUR
T1  - Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells
AU  - Emmanuel Reyes-Uribe
AU  - Octavio Galindo-Hernandez
AU  - Pedro Cortes-Reynosa
AU  - Eduardo Perez Salazar
Y1  - 2015/06/16
PY  - 2015
N1  - https://doi.org/10.11648/j.crj.20150303.12
DO  - 10.11648/j.crj.20150303.12
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 52
EP  - 62
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20150303.12
AB  - Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.
VL  - 3
IS  - 3
ER  -

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Author Information

Emmanuel Reyes-Uribe

Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Octavio Galindo-Hernandez

Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Pedro Cortes-Reynosa

Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico
Eduardo Perez Salazar

Departamento de Biologia Celular, Cinvestav-IPN. Mexico, Mexico

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APA Style

Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar. (2015). Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Research Journal, 3(3), 52-62. https://doi.org/10.11648/j.crj.20150303.12

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ACS Style

Emmanuel Reyes-Uribe; Octavio Galindo-Hernandez; Pedro Cortes-Reynosa; Eduardo Perez Salazar. Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Res. J. 2015, 3(3), 52-62. doi: 10.11648/j.crj.20150303.12

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AMA Style

Emmanuel Reyes-Uribe, Octavio Galindo-Hernandez, Pedro Cortes-Reynosa, Eduardo Perez Salazar. Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells. Cancer Res J. 2015;3(3):52-62. doi: 10.11648/j.crj.20150303.12

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@article{10.11648/j.crj.20150303.12,
  author = {Emmanuel Reyes-Uribe and Octavio Galindo-Hernandez and Pedro Cortes-Reynosa and Eduardo Perez Salazar},
  title = {Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells},
  journal = {Cancer Research Journal},
  volume = {3},
  number = {3},
  pages = {52-62},
  doi = {10.11648/j.crj.20150303.12},
  url = {https://doi.org/10.11648/j.crj.20150303.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20150303.12},
  abstract = {Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.},
 year = {2015}
}

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TY  - JOUR
T1  - Cell Migration Induced by Native Type IV Collagen Requires PI3K/Akt2 and EGFR Activity in MDA-MB-231 Breast Cancer Cells
AU  - Emmanuel Reyes-Uribe
AU  - Octavio Galindo-Hernandez
AU  - Pedro Cortes-Reynosa
AU  - Eduardo Perez Salazar
Y1  - 2015/06/16
PY  - 2015
N1  - https://doi.org/10.11648/j.crj.20150303.12
DO  - 10.11648/j.crj.20150303.12
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 52
EP  - 62
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20150303.12
AB  - Basement membrane (BM) is a specialized extracellular matrix (ECM) that separates epithelial cells from surrounding stroma and regulates various biological processes including morphology, growth, differentiation, adhesion and motility. Type IV collagen is the major component of BM, provides structural framework of all BMs and interacts with cell surface receptors including integrins and discoidin domain receptors (DDRs). The DDRs are receptor tyrosine kinases that get activated by collagens in their native triple-helical form and present sustained and slow activation kinetics. Particularly, DDR1 signaling mediates differentiation, immune response, migration and wound healing. However, the signal transduction pathways involved in cell migration induced by native IV collagen in breast cancer cells has been poorly studied. Here we demonstrate that native type IV collagen induces Akt2 and FAK activation through a DDR1, PI3K and epidermal growth factor receptor (EGFR)-dependent pathway in MDA-MB-231 breast cancer cells. In addition, cell migration induced by native type IV collagen requires PI3K, Akt2 and EGFR activity, whereas collagen IV also induces NFκB-DNA binding activity through a DDR1, PI3K, Akt2 and EGFR-dependent pathway. In summary, we demonstrate that migration induced by native type IV collagen requires PI3K/Akt2 and EGFR activity in MDA-MB-231 breast cancer cells.
VL  - 3
IS  - 3
ER  -

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