Evaluation of Anti-Cancer Properties of Lichens Using Albino wistar Rats as an Animal Model
Cancer Research Journal
Volume 4, Issue 6, November 2016, Pages: 84-89
Received: Oct. 20, 2016; Accepted: Nov. 4, 2016; Published: Nov. 29, 2016
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Authors
Shanmugam Poornima, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, India
Ponnusamy Ponmurugan, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, India
Khader Syed Zameer Ahmed, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, India
Ganesan Ayyappadasan, Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode, Namakkal District, Tamil Nadu, India
Fahad Khalid Aldhafiri, College of Applied Medical Sciences, Majmaah University, Al Majmaah, The Kingdom of Saudi Arabia
Balakrishnan Santhanaraj, College of Applied Medical Sciences, Majmaah University, Al Majmaah, The Kingdom of Saudi Arabia
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Abstract
Studies were undertaken to evaluate the efficacy of purified lichen extracts against cancer induced Albino wistar rats as an animal model under in vivo condition. Lichen species were collected from different mean sea level of Yercaud hills of Tamil Nadu, India. Extraction and purification of lichen compounds were done using silica gel column chromatography with TLC analysis. Different fractions were collected from the crude extract and it was subjected to study the potential of anticancerous property. Anticancer activity was confirmed that Parmotrema reticulatum exhibited the highest control over the cervical cancer in cell line model. The compound Benzoic acid, 2, 4 dihydroxy, 6 methyl-methyl ester was identified as a potent molecule from Rocella montagnei. Anticancer effect of P. reticulatum and P. hababianum and their compounds were further evaluated to have the effective cancer drug against cervical cancer disease. Cancer cell line induced rats showing a significant reduction tumor proliferation which were treated with bioactive anti-cancer lichen compounds in comparison with the standard drug. Histopathology and hematology studies have been done to examine to confirm the level of tumor growth in the animals and to analyze the blood platelet count in tumors; respectively.
Keywords
Cancer, Lichens, Parmotrema Reticulatum, Rocella Montagnei, Animal Model
To cite this article
Shanmugam Poornima, Ponnusamy Ponmurugan, Khader Syed Zameer Ahmed, Ganesan Ayyappadasan, Fahad Khalid Aldhafiri, Balakrishnan Santhanaraj, Evaluation of Anti-Cancer Properties of Lichens Using Albino wistar Rats as an Animal Model, Cancer Research Journal. Vol. 4, No. 6, 2016, pp. 84-89. doi: 10.11648/j.crj.20160406.11
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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