Cancer Research Journal

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Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer

Received: 02 February 2018    Accepted: 25 February 2018    Published: 02 April 2018
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Abstract

Unlike almost all the cervical, penile, vulvar, and anal cancers, where Human papilloma virus has long known to play a vital role, a causative link between carcinogenic Human papilloma virus and lung cancer have been found to be highly variable and contradictory. Data also shows geography and race-dependenty. Apart from etiological factors, viral carcinogen can manipulate the cell cycle, hamper cell apoptosis and also interrupt the cell division in host cell which lead to the lung cancer. Molecular studies of carcinogenic Human papilloma virus have found that E6/E7 acts as mitotic mutators which play an important role in pathogenicity and oncogenicity. Analysis of genome sequence of Human papilloma virus revealed that ORF having conserved in early region, E6 and E7 required for viral pathogenicity and oncogenicity can be the suitable target for RNAi technology. RNAi works by silencing or turning off gene expression to control pathogenicity and oncogenicity by blocking its replication processes. Therefore, the work is done on the basis of rational siRNA designing method by targeting viral oncogenic E6 and E7 genes of Human papilloma virus types16 & 18. Searching siRNA target sequences, multiple sequence alignment, forecasting secondary structure and RNA-RNA interaction prediction was done by various computational software tools for designing RNA-based therapeutics (siRNA). In this study, four effective siRNA were predicted rationally for oncogenic E6 and E7 genes of Human papilloma virus types 16 & 18 which might be used as a potential RNA based therapeutics to control the rate of carcinogenesis and degree of oncogenicity. The outcome of this study provides a basis of the researchers towards understating to development of RNA-based therapeutics (siRNA) at genomic level.

DOI 10.11648/j.crj.20180602.14
Published in Cancer Research Journal (Volume 6, Issue 2, June 2018)
Page(s) 62-69
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Lung Cancer, Human Papilloma Virus, RNA-Based Therapeutics, RNAi Technology

References
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Author Information
  • Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Chittagong, Bangladesh

  • Institute of Nuclear Medicine and Allied Sciences, Bangladesh Atomic Energy Commission, Cox’s Bazar, Bangladesh

  • Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong, Chittagong, Bangladesh

Cite This Article
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    Moinul Abedin Shuvo, Sayeedul Alam Prince, Arifuzzaman. (2018). Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer. Cancer Research Journal, 6(2), 62-69. https://doi.org/10.11648/j.crj.20180602.14

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    ACS Style

    Moinul Abedin Shuvo; Sayeedul Alam Prince; Arifuzzaman. Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer. Cancer Res. J. 2018, 6(2), 62-69. doi: 10.11648/j.crj.20180602.14

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    AMA Style

    Moinul Abedin Shuvo, Sayeedul Alam Prince, Arifuzzaman. Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer. Cancer Res J. 2018;6(2):62-69. doi: 10.11648/j.crj.20180602.14

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  • @article{10.11648/j.crj.20180602.14,
      author = {Moinul Abedin Shuvo and Sayeedul Alam Prince and Arifuzzaman},
      title = {Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer},
      journal = {Cancer Research Journal},
      volume = {6},
      number = {2},
      pages = {62-69},
      doi = {10.11648/j.crj.20180602.14},
      url = {https://doi.org/10.11648/j.crj.20180602.14},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.crj.20180602.14},
      abstract = {Unlike almost all the cervical, penile, vulvar, and anal cancers, where Human papilloma virus has long known to play a vital role, a causative link between carcinogenic Human papilloma virus and lung cancer have been found to be highly variable and contradictory. Data also shows geography and race-dependenty. Apart from etiological factors, viral carcinogen can manipulate the cell cycle, hamper cell apoptosis and also interrupt the cell division in host cell which lead to the lung cancer. Molecular studies of carcinogenic Human papilloma virus have found that E6/E7 acts as mitotic mutators which play an important role in pathogenicity and oncogenicity. Analysis of genome sequence of Human papilloma virus revealed that ORF having conserved in early region, E6 and E7 required for viral pathogenicity and oncogenicity can be the suitable target for RNAi technology. RNAi works by silencing or turning off gene expression to control pathogenicity and oncogenicity by blocking its replication processes. Therefore, the work is done on the basis of rational siRNA designing method by targeting viral oncogenic E6 and E7 genes of Human papilloma virus types16 & 18. Searching siRNA target sequences, multiple sequence alignment, forecasting secondary structure and RNA-RNA interaction prediction was done by various computational software tools for designing RNA-based therapeutics (siRNA). In this study, four effective siRNA were predicted rationally for oncogenic E6 and E7 genes of Human papilloma virus types 16 & 18 which might be used as a potential RNA based therapeutics to control the rate of carcinogenesis and degree of oncogenicity. The outcome of this study provides a basis of the researchers towards understating to development of RNA-based therapeutics (siRNA) at genomic level.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Prediction and Depiction of Potential RNA-Based Therapeutics for Oncogenic E6 and E7 Genes of Human Papilloma Virus Types 16 & 18: A New Class of Treatment for Lung Cancer
    AU  - Moinul Abedin Shuvo
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    AB  - Unlike almost all the cervical, penile, vulvar, and anal cancers, where Human papilloma virus has long known to play a vital role, a causative link between carcinogenic Human papilloma virus and lung cancer have been found to be highly variable and contradictory. Data also shows geography and race-dependenty. Apart from etiological factors, viral carcinogen can manipulate the cell cycle, hamper cell apoptosis and also interrupt the cell division in host cell which lead to the lung cancer. Molecular studies of carcinogenic Human papilloma virus have found that E6/E7 acts as mitotic mutators which play an important role in pathogenicity and oncogenicity. Analysis of genome sequence of Human papilloma virus revealed that ORF having conserved in early region, E6 and E7 required for viral pathogenicity and oncogenicity can be the suitable target for RNAi technology. RNAi works by silencing or turning off gene expression to control pathogenicity and oncogenicity by blocking its replication processes. Therefore, the work is done on the basis of rational siRNA designing method by targeting viral oncogenic E6 and E7 genes of Human papilloma virus types16 & 18. Searching siRNA target sequences, multiple sequence alignment, forecasting secondary structure and RNA-RNA interaction prediction was done by various computational software tools for designing RNA-based therapeutics (siRNA). In this study, four effective siRNA were predicted rationally for oncogenic E6 and E7 genes of Human papilloma virus types 16 & 18 which might be used as a potential RNA based therapeutics to control the rate of carcinogenesis and degree of oncogenicity. The outcome of this study provides a basis of the researchers towards understating to development of RNA-based therapeutics (siRNA) at genomic level.
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