Efficacy of Manual Liquid Based Cytology over Conventional Cytology in Oral Squamous Cell Carcinoma
Science Journal of Clinical Medicine
Volume 4, Issue 4-1, July 2015, Pages: 11-15
Received: Feb. 20, 2015;
Accepted: Feb. 23, 2015;
Published: Aug. 3, 2015
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Sudhir Mishra, Department of Oral Pathology and Microbiology, Rajiv Gandhi University, Belgaum, karnataka, India
Jagadish Hosmani, Department of Oral Pathology and Microbiology, Rajiv Gandhi University, Belgaum, karnataka, India
Nandini Manoli, Department of Pathology, JSS University, Mysore, karnataka, India
Ramakant Nayak, Department of Oral Pathology and Microbiology, Rajiv Gandhi University, Belgaum, karnataka, India
Manjunath G. V., Department of Pathology, JSS University, Mysore, karnataka, India
Context/Background: Oral cytology has come long way from its primitive Papanicolau days. Liquid Based cytology has shown significant advantages over conventional exfoliative cytology. However, LBC requires expensive automated devices and materials which might not be affordable for many cytopathological laboratories in countries with limited resources. Manual liquid Based Cytology (MLBC) is a technique that enables cells to be suspended in a monolayer and thus improves detection of lesions and improvement of adequacy. Aim: To study and evaluate the diagnostic efficiency and reliability of MLBC in comparison with conventional PAP smear (CPS) of oral squamous cell carcinoma. Materials and Methods: Two smear were prepared from 50 patients, clinically diagnosed with Oral Squamous Cell Carcinoma. Each smear was subjected to MLBC and CPS methods. The slides were evaluated by two pathologists for the staining characterstics of nucleus and cytoplasm. The diagnostic efficiency of each smear was evaluated by comparing the cytological diagnosis of each method with histopathological diagnosis. Results: Increased detection rate with MLBC was 29.41%. Identifying cellular atypia by MLBC was more sensitive (44%) compared to CPS (34%) with similar specificity (100%). The percentage agreement by the two methods was 77.28%. Conclusion: MLBC is an easy, cost effective technique comparable to CPS; however, it warrants further study in its potential application in screening of oral precancer and cancer.
Manjunath G. V.,
Efficacy of Manual Liquid Based Cytology over Conventional Cytology in Oral Squamous Cell Carcinoma, Science Journal of Clinical Medicine. Special Issue: Latest Different Concepts of Gynaecology.
Vol. 4, No. 4-1,
2015, pp. 11-15.
Fedele, S. 2009. Diagnostic aids in the screening of oral cancer. Head Neck Oncology. 1(5): doi:10.1186/1758-3284-1-5.
Neville, B.W., D.D. Damm, C.M. Allen, and J.E. Bouquot. 1995. Oral & maxillofacial pathology. Vol. 620: Saunders Philadelphia.
Pektas , Z.Ö., A. Keskin, Ö. Günhan, and Y. Karslioglu. 2006. Evaluation of nuclear morphometry and DNA ploidy status for detection of malignant and premalignant oral lesions: quantitative cytologic assessment and review of methods for cytomorphometric measurements. J Oral Maxillofacsurg64 (4):628-635.
Fedele S. Diagnostic aids in the screening of oral cancer. Head Neck Oncol. 2009;1:5
Acha A, Ruesga MT, Rodríguez MJ, Martínez de Pancorbo MA, Aguirre JM. Applications of the oral scraped (exfoliative) cytology in oral cancer and precancer. Med Oral Patol Oral Cir Bucal.2005;10:95-102.
Mashberg, A., and L.J. Feldman. 1988. Clinical criteria for identifying early oral and oropharyngeal carcinoma: Erythroplasia revisited+. Am J Surg 156 (4):273-275.
Maraki, D., J. Becker, and A. Boecking. 2004. Cytologic and DNA cytometric very early diagnosis of oral cancer. J Oral Pathol Med 33 (7):398-404.
Nandini NM, Nandish SM, Pallavi P, Akshatha SK, Chandrashekhar AP, Anjali S, Dhar M. Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings. Asian Pacific Journal of Cancer Prevention, 2012; 3645-3651
Hayama FH, Motta CF, Silva A, Migliari DA. Liquid-based preparations versus conventional cytology: specimen adequacy and diagnostic agreement in oral lesions. Med Oral Patol Oral Cir Buccal 2005;10:115-22.
Sherwani RK, Khan T, Aktar K, et al (2007). Conventional pap smear and liquid based cytology for cervical cancer screening-A comparative study. J Cytology, 24, 167-72.
Kavatkar AN, Nagwamshi CA, Dabaks M (2008). Study of a manual method of liquid based cervical cytology. Indian J Pathology and Microbiology, 59, 190-4.
Delavarian Z, Mohtasham N, Mozaffari P, Pakfetrat A, Shakeri M, Maddah R. Evaluation of the diagnostic value of a Modified Liquid-Based Cytology using OralCDx ® Brush in early detection of oral potentially malignant lesions and oral cancer. Med Oral Patol Oral Cir Buccal. 2010 Sep 1: 15 (5):e671-6.
Maksem JA, Finnomore M, Belsheim BL, et al (2001). Manual method for liquid based cytology. A demonstration using 1,000 Gynaecological cytologies collected directly to vial and prepared by a smear – slide technique. Diagn Cytopathology, 25, 334-8.
Rajendran R, Shivpathasundharam B. Shafer’s Textbook of Oral Pathology. 6th edition. New Delhi: Elsevier; 2009.
Mehrotra R. Oral cytology. 12th ed. Springer; 2013. p.5.
Sherwani RK, Khan T, Akhtar K, Zeba A, Siddiqui FA, Rahman K, Afsan N. Conventional Pap Smear and Liquid Based Cytology for Cervical Cancer Screening – A Comparative Study. Journal of Cytology 2007;24(4):167-172
Deshou H, Changhua W, Quiyan L, et al (2009). Clinical utility of LiquiPrep cytology system for primary cervical cancer screening in a large urban hospital setting in China. J Cytology, 26, 20-5.
Johnson T, Maksem JA, Belshein BL, et al (2000). Liquid based cervical cell collection with brushes and wooden spatulas. Diagn Cytopathol, 22, 86-91.
Vidal A K, Junior A, Mello R J, Brandão R A, Lima PA, Figueiroa J N. Conventional Cytology versus liquid-based cytology for prevention and early diagnosis of oral squamous cell carcioma (OSCC). Odontol. Clín.-Cient., Recife, 2011; 10 (1) 31 – 36
Navone R, Burlo P, Pich A, Pentenoro M, Broccoletti R, Marsico A et al. The impact of Liquid-based cytology on the diagnosis of oral squamous cell dysplasia and carcinoma. Cytopathology 2007;18:356–360.