Effect of Microbes, Npk Fertilizer and Cow Dung on the Biodegradation of Polycyclic Aromatic Hydrocarbons from Abattoir Wastes in Nigeria
International Journal of Environmental Monitoring and Analysis
Volume 1, Issue 1, February 2013, Pages: 1-14
Received: Dec. 29, 2012; Published: Feb. 20, 2013
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D. N. Ogbonna, Department of Applied and Environmental Biology, Rivers State University of Science and Technology, Nkpolu-Oroworukwo, Port Harcourt, Nigeria
T. J. K. Ideriah, Institute of Pollution Studies, Rivers State University of Science and Technology, Nkpolu-Oroworukwo, Port Harcourt, Nigeria
M. I. Nwachukwu, Department of Microbiology, Imo State University, Owerri
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The biodegradation of polycyclic aromatic hydrocarbons by associated microorganisms were studied. Soil and waste water samples were collected from four abattoirs located at Egbu and Ogbe in Imo state, Trans-Amadi and Ahoada in Rivers State. Likewise, surface water and sediment samples from Otamiri River and Oginigba Creek adjoined to Egbu and Trans-Amadi abattoirs, respectively were collected using standard methods. The ability of hydrocarbon utilizing bacteria and fungi to biodegrade polycyclic aromatic hydrocarbons (PAHs) was carried out by growing the isolates in a mineral salt broth amended with PAHs and nutrients (NPK fertilizer and cow dung). Levels of polycyclic aromatic hydrocarbons (PAHs) left after incubation were determined using Gas chromatographic method. Results showed that soil samples from various abattoirs had high concentrations of PAHs ranging from 0.176 mg/kg at Ahoada abattoir to 2.44 mg/kg at Egbu abattoir. In a 21-day biodegradation test, there was a drop in the initial concentration of PAHs used as control from 0.03 mg/l to 0.024 mg/l. There was loss of low molecular weight PAHs than the high molecular weight components. It is evident from the study that both mixed cultures of bacteria and fungi can biodegrade polycyclic aromatic hydrocarbons. Results from this study have shown that abattoir wastes have high pollution strength and thus should be treated before being discharged into the environment. Of note is the fact that the microorganisms isolated from the abattoirs are potential agents of remedying environments impacted by polycyclic aromatic hydrocarbons (PAHs).
Polycyclic Aromatic Hydrocarbons, Abattoir, Biodegradation, Microorganisms, NPK, Cow Dung
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D. N. Ogbonna, T. J. K. Ideriah, M. I. Nwachukwu, Effect of Microbes, Npk Fertilizer and Cow Dung on the Biodegradation of Polycyclic Aromatic Hydrocarbons from Abattoir Wastes in Nigeria, International Journal of Environmental Monitoring and Analysis. Vol. 1, No. 1, 2013, pp. 1-14. doi: 10.11648/j.ijema.20130101.11
Hughes, J B., Beckles, D M., Chandra, S D., Ward, C H. Utilization of bioremediation for the treatment of PAH-contaminated sediments. J. Ind. Microbiol. Biotech. 1997, 18: 152-160.
Okafor, E. Ch., Opuene, K. Preliminary assessment of trace metals and polycyclic aromatic hydrocarbons in the sediments. Int. J Environ Sci Tech 2007, 4(2): 233-240.
Fagbote, E O., Olanipekun, E O. Levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in sediment of bitumen deposit impacted area. Int. J Environ Sci Tech 2010, 7(3): 561-570.
Lee, K H., Byeon, S H. The biological monitoring of urinary 1hydroxypyrene by PAH exposure among smokers. Int. J Environ Res. 2010, 4(3):439-442.
Keith L H, Telliard W A,. Priority pollutants I. A perspective view. Environmental Science and Technology, 1979, 13(4):416–423.
Eibes G, Cajthaml T, Moreira M T, Feijoo G, Lema J M,. Enzymatic degradation of anthracene, dibenzothiophene and pyrene by manganese peroxidase in media containing acetone. Chemosphere, 2006, 64(3): 408–414.
Ockenden W A, Breivik K, Meijer S N, Steinnes E, Sweetman AJ, Jones K C,. The global re-cycling of persistent organicpollutants is strongly retarded by soils. EnvironmentalPollution, 2003, 121(1): 75–80.
Mohan S V, Kisa T, Ohkuma T, Kanaly R A, ShimizuY,. Bioremediation technologies for treatment ofPAH-contaminated soil and strategies to enhance process efficiency. Reviews in Environmental Science and Biotechnology, 2006, 5(4): 347–374.
Yu H, Huang G H, An C J, Wei J,. Combined effects of DOM extracted from site soil/compost and biosurfactant onthe sorption and desorption of PAHs in a soil-water system.Journal of Hazardous Materials, 2011, 190(1-3): 883–890.
Osuji, L C ., Ezebuiro, P E. Hydrocarbon contamination of a typical mangrove floor in Niger Delta, Nigeria. Int. J Environ Sci Tech. 2006, 3(3): 313-320.
Perugini, M., Visciano, P., Giammarino, A., Manera, M., Nardo, W D., Amorena, M. Polycyclic aromatic hydrocarbons in marine organisms from the Adriatic Sea Italy. Chemosphere 2007, 66(10): 1904-1910.
Wilson, S C and Jones, K C,. Bioremediation of soil contaminated with polynuclear aromatic hydrocarbons (PAHs). A Review. Environ. Pollut. 1993, 81: 229-249.
Cerniglia, C. E,. Biodegradation of polycyclic aromatic hydrocarbons. Curr. Opin. Biotech., 1993, 3 (2-3), 331-338
McElroy, A.E., Farrington, J W., Teal, J M. Degradation of PAHs by microorganisms. Appl. Environ. Microbiol. 1989, 55: 269-274.
Cho, J.C, Kim, S J. Biodegradation of phenanthrene in soil microcosms. In: Int. In-situ and On-site Bioremediation Symp. New Orleans. 1997, Pp. 105-110.
Harms, H., Bosman, T.N.P.. Mass transfer limitation of microbial growth and pollutant degradation. J. Indust. Microbiol. Biotech. 1997, 18: 97-105.
Debosz K, Petersen S O, Kure L K, Ambus P,. Evaluating effects of sewage sludge and household compost on soil physical, chemical and microbiological properties. Applied Soil Ecology, 2002, 19(3): 237–248.
Celik I, Ortas I, Kilic S,. Effects of compost, mycorrhiza, manure and fertilizer on some physical properties of a Chromoxerert soil. Soil & Tillage Research, 2004, 78(1): 59–67.
Courtney R G, Mullen G J,. Soil quality and barley growth as influenced by the land application of two compost types. Bioresource Technology, 2008, 99(8): 2913–2918.
Alvarez-Bernal D, Garc´ıa-D´ıaz E L, Contreras-Ramos S M,Dendooven L,. Dissipation of polycyclic aromatic hydrocarbonsfrom soil added with manure or vermicompost.Chemosphere, 2006, 65(9): 1642–1651.
Contreras-Ramos S M, Alvarez-Bernal D, Dendooven L,. Removal of polycyclic aromatic hydrocarbons from soilamended with biosolid or vermicompost in the presence of earthworms (Eisenia fetida). Soil Biology and Biochemistry, 2008, 40(7): 1954–1959.
Fernan dez-Luqueno, F., Marsch, R., Espinosa-Victoria. D., Thalasso, F., Lara, M E H. Remediation of PAHs in a saline-alkaline soil amended with wastewater sludge and the effect on dynamics of C and N. Science of the Total Environment. 2008, 402 (1): 18-28.
Simeon N, Mercier G, Blais J F, Ouvrard S, Sauvage C, RenatJ C,. Decontamination of contaminated soilsby polycyclic aromatic hydrocarbons in the presence ofsupplementary organic structures. Journal of Environmental Engineering and Science, 2008, 7: 467–479.
Kobayashi T, Navarro R R, Tatsumi K, Iimura Y,. Influenceof compost amendment on pyrene availability fromartificially spiked soil to two subspecies of Cucurbita pepo. Science of the Total Environment, 2008, 404(1): 1–9.
ejada M, Masciandaro G,. Application of organic wasteson a benzo(a)pyrene polluted soil. Response of soilbiochemical properties and role of Eisenia fetida. Ecotoxicologyand Environmental Safety, 2011, 74(4): 668–674.
Bouchez, M., Blanchet, D. ,Vandecas-Teele, J.P. Degradation of polycyclic aromatic hydrocarbons by pure strain and by defined strain association: Inhibition phenomena and co-metabolism. Appl. Microbiol. Biotechnol. 1995, 43: 156-164.
Foght, J.M., Gutnick, D.L., Westlake, D.W.S. Effect of emulsion on biodegradation of crude oil in pure and mixed cultures. Appl. Environ. Microbiol. 1989, 55: 36-42.
Mueller, J.G., Chapman, P.J., Pritchard, P.H. Action of a fluoranthene-utilizing bacterial community on polycyclic aromatic hydrocarbon components of creosote. Appl. Environ. Microbiol. 1989, 55: 1504-1506.
Park, K.S., Sims, R.C., Dupont, R. The fate of the PAH compounds in two soil types: Influence of volatalization of abiotic loss and biological activity. Environ. Toxicol. Chem. 1990, 9(2): 187-196.
Miyachi, N., Tanaka, T., Suzuki, T., Hotta, Y., Omori, T. Microbial oxidation of dimethylnaphthalene isomers. Appl. Environ.Microbiol. 1993, 59: 1938-1942.
Sanseverino, J., Applegate, B.M., King, J.M.H., Sayler, G S. Plasmid-mediated mineralization of naphthalene, phenanthrene and anthracene. Appl. Environ. Microbiol. 1993, 59: 1931-1937.
Menn, F.M., Applegate, B.M., Soyler, G.S.. NAH catabolism of anthracene and phenanthrene to naphtholic acids. Appl. Environ. Microbiol. 1993, 59: 1938-1942.
Denome, S.A., Stanley, D.C., Olson, E.S., Young K.D. Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains: complete DNA sequence of the upper naphthalene catabolic pathway. J. Bacteriol. 1994, 175: 6890-6901.
Leahy, G.J. and Colwell, R.R. Microbial degradation of hydrocarbon in the environment. Microbiol. Rev. 1990, 54(3): 303-315.
Dibble, J.T., Bartha, R. Effect of environmental parameters on the biodegradation of oil sludge. Appl. Environ. Microbiol. 1985, 37: 729-739.
Odokuma, L.O., Okpokwasili, G.C. Seasonal influences on inorganic anion monitoring of the New Calabar River, Nigeria. Environ. Manage. 1993, 17(4): 491-496.
Onyeagba, R.A., Umeham, S.N. Analytical Methods in Water Microbiology. In: Onyeagba, R.A. (ed.). Laboratory Guide for Microbiology. 1st ed. Crystal Publishers Okigwe, Nigeria. 2004, Pp. 178-191.
Adesemoye, A.O., Opere, B.O. and Makinde, S.C.O. Microbial content of abattoir waste water and its contaminated soil in Lagos, Nigeria. Afr. J. Biotechnol. 5(20): (2006), 1963-1968.
Prescott, L.M., Harley, J.P., Klein, D.A. Microbiology. 6th ed. McGraw Hill, London. 2005, Pp. 135-140.
Holt, JG; Kreig, N; Sneath, P A H., Williams, S T. Bergey’s Manual of Determinatve Bacteriology. 9th edition. Williams and Wilkins Co., Baltimore, USA. 1994.
Barnett, H L., Hunter, B B. Illustrated Genera of Imperfect Fungi. 3rd ed. Burgess publishing company, Minnesota, USA. 1972.
Wang, W. Response of Nitrobacter sp. to toxicity. Environ. Int. 1984, 10 : 21-26.
Okerentugba, P O., Ezeronye, O U. Petroleum-degrading potentials of single and mixed microbial cultures isolated from rivers and refinery effluents in Nigeria. Afr. J. Biotechnol. 2003, 2(9): 288-292.
Okoro, C C. Biodegradation of hydrocarbons in untreated produce water using pure fungal cultures. Afr. J. Microbiol. Res. 2008, 2: 217-223.
Hedlund, B P., Geiselbrecht, A D., Bair, T J, Staley, J T. Polycyclic aromatic hydrocarbons degradation by a new marine bacterium, Neptunomonas sp. Appl. Environ. Microbiol. 1999, 65(1): 251-259.
Rothermich,M M., Hayes, L A., Lovley, D R. Anaerobic, sulphate-dependent degradation of polycyclic aromatic hydrocarbons in petroleum-contaminated habour sediment. Environ. Sci. Technol. 2002, 36: 4811-4817.
Andrea, R C., Tania, A.A., Lucia, R D.. Biodegradation of polycyclic aromatic hydrocarbons by soil fungi. Braz. J. Microbiol. 2001, 32(4) : 124-129.
Gadd, G M. Fungi in Bioremediation. Cambridge University Press, Cambridge. 2001, P. 220
Sutherland, J B. Degradation of hydrocarbons by yeast and filamentous fungi. Fungial Biotechnology in Agricultural Food and Environmental Application. Arora, D.K(ed). Marcel Dekker Inc., USA. 2004, P. 324.
[Haritash, A K., Kalushik, C P. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): A review. J. Hazard. Mat. 2009., 169: 1-15
Hamamura, N., Olson, S H., Ward, D M., Inskeep, W P. Microbial population dynamics associated with crude oil biodegradation in diver2001se soil. Appl. Environ. Microbiol. 2006, 72(9): 6316-6324.
Foght, J M., Westlake, D W S. Degradation of polycyclic aromatic hydrocarbons (PAHs) and aromatic heterocycles by a Pseudomonas sp. Can. J. Microbiol. 1988, 34: 1135-1141
Okpokwasili, G C., Sommerville, C C., Sullivan, M., Grimes, D J., Colwell, R R. Plasmid-mediated degradation of hydrocarbons in estuarine bacteria. Oil Chem. Pollut. 1986, 3:177-129.
Jing Z., Xiangui L., Weiwei L., Yiming W., Jun Z., Hong C. Effect of organic wastes on the plant-microbe remediation for removal of aged PAHs in soils. Journal Environ Sciences 2012, 24(8): 1476-1482.
Adegunloye, D V., Adetuyi, F C., Akinyosoye, F A., Doyeni, M O. Microbial analysis of compost using cow dung as booster. Pak J Nutr 6(5): 2007, 506-510.
Akinde, S B ., Obire, O. Aerobic heterotrophic bacteria and petroleum- utilizing bacteria from cow dung and poultry \manure. World J Microbiol Biotechnol 2008, 24: 1999-2002.
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