β-N-Acetylhexosaminidase (NAHA) as a Marker of Fungal Cell Biomass – Storage Stability and Relation to β-Glucan
International Journal of Environmental Monitoring and Analysis
Volume 3, Issue 4, August 2015, Pages: 205-209
Received: May 18, 2015;
Accepted: Jun. 1, 2015;
Published: Jun. 14, 2015
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Ragnar Rylander, BioFact Environmental Health Research Centre, Lerum, Sweden
Background. Laboratory and field studies have demonstrated that the enzyme β-N-acetylhexosaminidase (NAHA) is a marker of fungal biomass. The purposes of this study was to determine, 1) the stability of the NAHA enzyme on stored filters, 2) the effect of air movement during the sampling of particles and resulting NAHA enzyme activity, and 3) the relationship between enzyme activity and β-glucan concentration. Methods. Replicate air, filtered (0.8 µm pore, cellulose acetate) samples were obtained and stored at room temperature. Then 7 to 12 samples were analysed at 0, 10, 20, 30 or 360 days. Air samples were collected in rooms with no activity, walking or with fan use. The NAHA activity on the filters was measured by adding an enzyme activator and the fluorescence was measured. The β-glucan concentrations were measured using a Limulus-based test with and without solubilisation with NaOH. Results. Storage of filters up to 360 days did not influence the content of NAHA. Movements in the room increased the NAHA values but agitation in terms of fan blowing did not increase the potential to detect differences between rooms with or without fungal growth. There was a relation between NAHA and the non-soluble fraction of β-glucan. Comments: Enzyme measurements of fungal biomass are rapid and easy to perform. The sensitivity and specificity of the method is high which makes it suitable for field use. Incorporation of the small fungal fractions in exposure assessment is important from a health point of view as they have a higher penetration rate into the deep parts of the lung. Summary: The evaluation of the enzyme method to determine fungal growth further supports the relevance of this method to relate to medical effects of fungal exposure.
β-N-Acetylhexosaminidase (NAHA) as a Marker of Fungal Cell Biomass – Storage Stability and Relation to β-Glucan, International Journal of Environmental Monitoring and Analysis.
Vol. 3, No. 4,
2015, pp. 205-209.
Singh J. Building mycology. E&FN Spoon London 1994, pp 1 – 326.
Mendell MJ, Mirer AG, Cheung K, Tong M, Douwes J. Respiratory and allergic health effects of dampness, mold, and dampness-related agents: a review of the epidemiologic evidence. Env Health Persp 2011,119,748-756.
Sauni R, Uitti J, Jauhiainen M, Kreiss K, Sigsgaard T, Verbeek JH. Remediating buildings damaged by dampness and mould for preventing or reducing respiratory tract symptoms, infections and asthma (Review) Evid Based Child Health 2013; 3:944-1000.
Quansah R, Jaakkola MS, Hugg TT, Heikkinen SA, Jaakkola JJ. Residential dampness and molds and the risk of developing asthma: systematic review and meta-analysis. PLoS One 2012; 11: e47526.doi:10.137 t/
Reponen T, Seo S-C, Grimsley F et al. Fungal fragments in moldy houses: a field study in homes in New Orleans and Southern Ohio. Atmos Environ 2007,41,8140-8149 .
Rylander R. Organic dust induced pulmonary disease – the role of mould derived β-glucan. Ann Agr Env Med 2010,17,9-13 .
Seo S-C, Reponen T, Levin L, Grinshpur SA. Size-fractionated (1-3)-β-D-glucan concentrations aerosolized from different moldy building materials. Sci Tot Env 2009,407,806-810 .
Adhikan A, Reponen T. Rylander R. Airborne fungal cell fragments in homes in relation to total fungal biomass. Indoor Air 2013, 23,142-147 .
Madsen AM. NAGase activity in airborne biomass dust and relationship between NAGase concentration and fungal spores. Aerobiol 2003, 9,97-105.
Reeslev M, Miller M, Nielsen KF. Quantifying mold biomass on gypsum board: comparison of ergosterol and beta-N-acetylhexosaminidase as mold biomass parameters. Appl Environ Microbiol 2003, 69,3996-3998.
Rylander R, Reeslev M, Hulander T J. Airborne enzyme measurements to detect indoor air mould exposure. Environ Monit 2010,12,2161-2164.
Reeslev M, Nielsen JC, Miller M, Rogers L. Aggressive sampling – improving the predicted value of air sampling for fungal aerosols. Manuscript
Holst G, Host A, Doekes G, Meyer HW, Madsen AM, Sigsgaard T. Determinants of house dust endotoxin and β-(1-3)-D-glucan in houses of Danish children. Indoor Air 2015; 25:245-249.
Terčelj M, Salobir B, Rylander R. Fungal exposure in homes of patients with sarcoidosis – an environmental exposure study. Env Health 2011,10,8-13.
Terčelj M, Salobir B, Narancsik Z, Kriznar K, Grezetic-Romcevic T, Matos T, Rylander R. Nocturnal asthma and domestic exposure to fungi. Indoor+Built Env 2013,22,876-880.
Terčelj M, Salobir B, Zupancic M, Wraber B. Rylander R. Inflammatory markers and pulmonary granuloma infiltration in sarcoidosis. Respirology 2014,19,225- 230 .
Terčelj M, Stopinšek S, Ihan A, Salobir B, Simčič S, Rylander R. Serum IL-10 in patients with sarcoidosis is supressed at high levels of fungal exposure. Pulm Med, 2014,dx.doi.org/10.1155)2014/164565.