Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment
International Journal of Environmental Monitoring and Analysis
Volume 3, Issue 4, August 2015, Pages: 233-237
Received: Feb. 20, 2015;
Accepted: Jul. 14, 2015;
Published: Aug. 19, 2015
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Ambreen Gul, Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Asma Siddique, Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Quratulain Syed, Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Muhammad Nadeem, Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
Shahjahan Baig, Food and Biotechnology Research Centre, Pakistan Council of Scientific and Industrial Research Laboratories Complex, Lahore, Pakistan
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The High Gravity (HG) ethanol fermentation at high temperature is very attractive and promising technology for fuel ethanol production. This study was designed to improve the osmotic as well as thermal behavior of the Saccharomyces cerevisiae strain isolated from distillery waste. Therefore, initial pH and substrate concentrations were optimized for this strain. The S. cerevisiae was subjected to thermal treatment to improve its fermentation ability without significant yield losses. At pHo 5.0, 95g/L ethanol was produced with the productivity (Qp) value of 1.02. The activation energy Ea value calculated at 30-40oC was 16.48kcal/mol indicating the thermal tolerance of the strain SC36. The results of glucose optimization revealed that at 250g/L glucose concentration, Qp, Yp/s and Yp/x value of 1.53g/Lh, 0.443g/g substrate and 41.4g/g biomass were obtained. The strain’s potential to be able to ferment very high gravity medium is very promising for fuel ethanol production
Thermal Treatment, Saccharomyces cerevisiae, Monod Model, High Gravity, Thermotolerant
To cite this article
Osmotic stress Response of Saccharomyces cerevisiae under HG and Elevated Temperature Environment, International Journal of Environmental Monitoring and Analysis.
Vol. 3, No. 4,
2015, pp. 233-237.
Bai F, Anderson W, Moo-Young M (2008). Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol. Adv. 26:89-105.
Bennett C (1971). Spectrophotometric acid dichromate method for the determination of ethyl alcohol. Am. J. Med. Technol. 37:217-220.
Casey GP, Magnus CA, Ingledew W (1984). High-gravity brewing: effects of nutrition on yeast composition, fermentative ability, and alcohol production. Appl. Environ.Microbiol. 48:639-646.
Dechant R, Saad S, Ibáñez AJ, Peter M (2014). Cytosolic pH regulates cell growth through distinct GTPases, Arf1 and Gtr1, to promote Ras/PKA and TORC1 activity. Mol. Cell. 55:409-421.
Dombek K, Ingram L (1987). Ethanol production during batch fermentation with Saccharomyces cerevisiae: changes in glycolytic enzymes and internal pH. Appl. Environ.Microbiol. 53:1286-1291.
Edgardo A, Carolina P, Manuel R, F J, B J (2008). Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production. Enz. Microb. Technol. 43:120-123.
Jones A, Ingledew W (1994). Fuel Alcohol Production: Optimization of Temperature for Efficient Very-High-Gravity Fermentation. Appl. Environ. Microbiol.1048-1051.
Lei J, Zhao X, Ge X, Bai F (2007). Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution. J.Biotechnol. 131:270-275.
Leudeking RP, Edgard L (1959). A Kinetic Study of the Lactic Acid Fermentation. Batch Process at Controlled pH. J.Biochem. Microbial. Technol. 1:393-412.
Magesh A, Preetha B, Viruthagiri T (2011). Simultaneous Saccharification and Fermentation of tapioca stem var. 226 white rose to ethanol by cellulase enzyme and Saccharomyces cerevisiae. Int. J. Chem. Tech. Research. 3:1821-1829.
Miller GL (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical chemistry. 31:426-428.
Oghome P, Kamalu C (2012). Kinetics of Ethanol Production FromNypa Palm (Mangroves Palm) Through Fermentation Process.
Ohta K, Wijeyaratne SC, Hayashida S (1988). Temperature-sensitive mutants of a thermotolerant yeast, Hansenulapolymorpha. J. Ferment. Technol. 66:455-459.
Ortiz‐Muñiz B, Carvajal‐Zarrabal O, Torrestiana‐Sanchez B, Aguilar‐Uscanga MG (2010). Kinetic study on ethanol production using Saccharomyces cerevisiae ITV‐01 yeast isolated from sugar cane molasses. J. Chem. Technol.Biotechnol. 85:1361-1367.
Pilone G (1984). Determination of ethanol in wine by titrimetric and spectrophotometric dichromate methods: collaborative study. J. Assoc. Off. Anal. Chem. 68:188-190.
Piper PW (1995). The heat shock and ethanol stress responses of yeast exhibit extensive similarity and functional overlap. FEMS Microbiol. Lett. 134:121-127.
Pramanik K, Rao DE (2005). Kinetic study of ethanol fermentation of grape waste using Saccharomyces cerevisiae yeast isolated from toddy. J. Inst. Eng. 85:53-58.
Roukas T, Lazarides H, Kotzekidou P (1991). Ethanol production from deproteinized whey by Saccharomyces cerevisiae cells entrapped in different immobilization matrices. Milchwissenschaft. 46:438-441.