Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer
International Journal of Environmental Monitoring and Analysis
Volume 4, Issue 1, February 2016, Pages: 21-26
Received: Nov. 7, 2015;
Accepted: Nov. 19, 2015;
Published: Feb. 1, 2016
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Stephen Emmanuel, Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Okwute Loretta Ojonoma, Department of Microbiology, University of Abuja, Gwagwalada- Abuja, Nigeria
Idoko Peter Arome, Department of Microbiology, Kogi State University, Anyigba-Kogi State, Nigeria
Makolo Daniel, School of Preliminary Studies, Kogi State Polytechnic, Kogi State, Nigeria
Biodegradation of mechanic workshop polluted soil amended with 2.4kg lime (Confluence fertilizer) was studied for a period of eight weeks (56 days). This was done by physicochemical analysis of the soil samples and microbiological analysis by spread plate inoculation on Nutrient agar (NA). The results revealed higher bacteria population in amended soil (AS) compared to oil free soil (OFS) and polluted soil (PS). The range of bacterial counts was Oil free soil (1.6×104 – 6.8 ×104 cfu/g), polluted soil (2.2×104 – 9.8×104 cfu/g) and Amended soil (1.62×104 – 1.12×105 cfu/g). The fungi counts range were Oil free soil (2.5×103 – 3.0×104 cfu/g), polluted soil (2.2×103 – 4.0×104 cfu/g) and Amended soil (2.1×103 – 2.7×103/sup> cfu/g). Eleven (11) organisms were isolated in the course of this study. The bacteria isolated were Pseudomonas putida, Bacillus subtilis, Micrococcus luteus, Proteus mirabilis and Staphylococcus aureus while the fungi isolated include Mucor mucedo, Candida tropicalis, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Rhizopus nigricans. The moisture content (MC), organic carbon, organic matter, pH and nitrate were higher in amended soil compared to unpolluted soil and polluted soil. The result of the study shows that lime (confluence fertilizer) can be used to enhanced biodegradation of oil polluted soil.
Okwute Loretta Ojonoma,
Idoko Peter Arome,
Study on Biodegradation of Mechanic Workshop Polluted Soil Amended with Lime Fertilizer, International Journal of Environmental Monitoring and Analysis.
Vol. 4, No. 1,
2016, pp. 21-26.
Ismail, S. (2008). Bioremediation Laboratory Manual. Department of Biotechnology, Islamic University, Gaza, Israel, 8-15.
Stephen, E., Emmanuel, O.E., Okpanachi, O.S., Emmanuel, S., Temola, O.T., Musa, K. and Ebiloma, I.P. (2013a). In-vitro study of biodegradation of spent lubricating oil by Aspergillus niger. Nature and Science, 11 (10): 40-44.
Stephen, E., Job, O.S. and Abioye, O.P. (2013b). Study on biodegradation of Diesel contaminated soil amended with cowpea chaff. Journal of Science & Multidisciplinary Research, 2(1):14-18.
Anene, M. and Chika, N. (2006). Studies on the bio-utililization of some petroleum hydrocarbons by single and mixed cultures of some bacterial species. African Journal of Microbiology Researchers, 5(12): 1457-1466.
George, J.S. and Metting, Jr. (1993). Soil Microbial Ecology. Application in Agricultural Environmental Management. Mercel Dekker., Inc. New York.
Cooney, J.J., Silver, S.A. and Beck, E.A. (1985). Factors influencing hydrocarbon egradation in three freshwater lakes, Microbial Ecology, 11(2): 127-137.
Barathi, S. and Vasudevan, N. (2001). Utilization of petroleum hydrocarbons by pseudomonas fluorescens isolated from a petroleum–contaminated soil. Environmentalist, 26(5-6): 413 -416.
Rahman, K.S.M, Kourkoutas, Y., Petsas, I., Marchant. R. and Banat, I.M. (2003). Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with Rhamnolipid and Micronutrients. Bioresource Technology, 90 (20): 159-168.
Brooijmans, R.J.W., Pastink, M.I and Siezen, R.J. (2009). Hydrocarbon-degrading bacteria: The oil-spill cleanup crew, Microbial Biotechnology, 2(6): 587-594.
Yakimov, M.M., Timmis, K.N. and Golyshin, P.N. (2007). Obligate oil-degrading marine bacteria. Current Opinions in Biotechnology, 18(3): 257-266.
Throne-Holst, M., Wentzel, A., Ellingsten, T.E., Kotlar, H.K. and Zotcher, S. B. (2007). Identification of novel genes involved in long-chain n-alkane degradation by Acinetobacteria sp. Strain DSM 17874, Applied and Environmental Microbiology, 73 (10): 3327-3332.
Chailan, F., Le Fleche, A., Bury, E., Phantavog, Y.H., Grimont, P., Saliot, A. and Oudot, J. (2004), Identification and biodegradation potential of tropical aerobic hydrocarbon – degrading micro-organisms, Research in Microbiology. 15(7): 587-595.
Singh, H. (2006). Mycoremediation: Fungal Bioremediation, Wiley-Interscience, New York, NY, USA.
Boguslawska- was, E. and Dabrowski W. (2001). The seasonal variability of Candida tropicalis and Candida tropicalis- like organisms in water and bottom sediment of the Szczecin lagoon, International Journal of Hygiene and Environmental Health, 203(5-6): 451-458.
Gupta, U.C. and Gupta, S.C. (1998).Trace Element Toxicity Relationship to Crop Production, Livestock and Human Health. Comm. Soil Science. Plant Anal. 29: 1491-1522.
Lin, J. and Mandri, T. (2007). Isolation and Characterization of engine oil degrading Indigenous microorganisms in Kwazulu-Natal, African Journal of Biotechnology. 6(1): 23-27.
Akunne, I. (2007). Effects of Oil Spillage on Farm Land (A Case Study of Uroboh). Department of Agricultural and Bioresources Engineering, University of Nigeria, Nsukka, Nigeria.
Hagwell, L.S., Delfino, L.M. and Rao, J.J. (1992). Partitioning of polycyclic aromatic hydrocarbon from oil into water, Environmental Science and Technology. 26(11): 2104- 2110.
Chesworth, W. (2008). Encyclopedia of Soil Science. Springer The Netherlands.
Moore, S. (2006). How does lime affect soil? http://homeguides. Sfgate.com/lime-affact-soil- 72806.html. Retrieved on the 5th January, 2015.
Omole, I.A. and Stephen, E. (2014). Antibiogram profile of bacteria isolated from wound infection of patients in three hospitals in Anyigba, Kogi State, Nigeria. FUTA Journal of Research in Sciences, 2: 258 – 266.
Public Health England. (2014). Preparation of samples and dilutions, plating and sub-culture. Microbiology Services. Food, Water and Environmental Microbiology Standard Method FNES26 (F2). Version 1.
Ibitoye, A.A. (2006). Laboratory Manual on Basic Soil Analysis (2nd ed). Foladave Nigeria Limited, Akure, pp 30-37.
Association of official Analytical Chemist. (AOAC, 2005). Methods of Analysis, Washington D.C.
Soil Survey Laboratory. (1996). Soil survey laboratory methods manual. Soil survey investigations report no. 42. Ver. 3.0. USDA-NRCS, Lincoln, NE.
Akinsanmi O. (1975). Certificate Agricultural Science. Longman, Nigeria, 104-112.
Okerentugba, P.O. and Ezeronye, O. U. (2003). Petroleum Degrading Potential of Single and Mixed Microbial Cultures Isolated From Rivers and Refinery Effluents in Nigeria. African Journal of Biotechnology, 2 (9): 288-292.
Eze, V.C. and Okpokwasili, G.C. (2010). Microbial and other related changes in a Niger Delta River sediment receiving industrial effluents. Continental Journal of Microbiology, 4: 15 – 24.
Okwute, O.L. and Ijah, U.J.J. (2014). Bioremediation of Palm oil mill effluent (POME) polluted soil using microorganisms found in Organic Wastes. The International Journal of Biotechnology, 3 (3): 32-46.
Atlas, R. M. and Cerniglia, C. E. (1995). Bioremediation of petroleum pollutants. Biological Science; 45(5): 332-350.
Stephen, E. and Temola, O.T. (2014). Enhanced biodegradation of spent lubricating oil contaminated soil using poultry litter. British Biotechnology Journal 4(8):868-876.
Osazee, E., Shehu, K. and Yerima, M.B. (2014). Physicochemical properties of oil contaminated soil as influenced by cow dung. Annals of Biological Sciences 2(4): 51-55.
Mbah, C.N., Nwite, J.N., and Okporie, O.E. (2006). Effect of organic waste on some physicochemical properties and productivity of spent oil ultisol in Abakaliki, Nigeria. Nigerian Journal of Tropical Agriculture 8:51-56.