Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent
American Journal of Environmental Protection
Volume 5, Issue 2, April 2016, Pages: 29-34
Received: Feb. 8, 2016; Accepted: Feb. 24, 2016; Published: Mar. 30, 2016
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Authors
Enimie Endurance Oaikhena, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Dominic Bawa Makaije, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Samuel Dangmwan Denwe, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Muhammad Muktar Namadi, Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria
Ali Ahmed Haroun, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
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Abstract
Five heavy metals tolerant bacteria were isolated from petroleum refinery effluent and identified as Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Proteus vulgaris and Klebsiella pneumoniae. Each isolate was inoculated into different concentrations of cadmium, chromium, nickel and zinc to determine its maximum tolerance for each heavy metal. All five isolates had low maximum tolerance concentration for cadmium (0.9 mg/L) when compared to chromium (5 mg/L), nickel (5 mg/L) and zinc (7 mg/L). Mixed culture consortium (MCC) remediated high percentage of cadmium (100%), chromium (33.4%), nickel (73.9%), and zinc (90.1%) from the petroleum refinery effluent than pure culture isolates. Among the pure culture isolates Pseudomonas aeruginosa (Cd (100%), Cr (23.1%), Ni (64.3%) & Zn (53.9%) yielded high values for the reduction of heavy metals in the refinery effluent when compared to Staphylococcus aureus, E.coli, Proteus vulgaris and Klebsiella pneumoniae. The isolated bacteria were effective for the remediation of heavy metals from petroleum refinery effluent.
Keywords
Bioremediation, Heavy Metal, Bacteria, Refinery, Petroleum
To cite this article
Enimie Endurance Oaikhena, Dominic Bawa Makaije, Samuel Dangmwan Denwe, Muhammad Muktar Namadi, Ali Ahmed Haroun, Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent, American Journal of Environmental Protection. Vol. 5, No. 2, 2016, pp. 29-34. doi: 10.11648/j.ajep.20160502.12
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Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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