Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent
American Journal of Environmental Protection
Volume 5, Issue 2, April 2016, Pages: 29-34
Received: Feb. 8, 2016;
Accepted: Feb. 24, 2016;
Published: Mar. 30, 2016
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Enimie Endurance Oaikhena, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Dominic Bawa Makaije, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Samuel Dangmwan Denwe, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Muhammad Muktar Namadi, Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria
Ali Ahmed Haroun, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
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Five heavy metals tolerant bacteria were isolated from petroleum refinery effluent and identified as Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Proteus vulgaris and Klebsiella pneumoniae. Each isolate was inoculated into different concentrations of cadmium, chromium, nickel and zinc to determine its maximum tolerance for each heavy metal. All five isolates had low maximum tolerance concentration for cadmium (0.9 mg/L) when compared to chromium (5 mg/L), nickel (5 mg/L) and zinc (7 mg/L). Mixed culture consortium (MCC) remediated high percentage of cadmium (100%), chromium (33.4%), nickel (73.9%), and zinc (90.1%) from the petroleum refinery effluent than pure culture isolates. Among the pure culture isolates Pseudomonas aeruginosa (Cd (100%), Cr (23.1%), Ni (64.3%) & Zn (53.9%) yielded high values for the reduction of heavy metals in the refinery effluent when compared to Staphylococcus aureus, E.coli, Proteus vulgaris and Klebsiella pneumoniae. The isolated bacteria were effective for the remediation of heavy metals from petroleum refinery effluent.
Bioremediation, Heavy Metal, Bacteria, Refinery, Petroleum
To cite this article
Enimie Endurance Oaikhena,
Dominic Bawa Makaije,
Samuel Dangmwan Denwe,
Muhammad Muktar Namadi,
Ali Ahmed Haroun,
Bioremediation Potentials of Heavy Metal Tolerant Bacteria Isolated from Petroleum Refinery Effluent, American Journal of Environmental Protection.
Vol. 5, No. 2,
2016, pp. 29-34.
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/
) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aneja K R (2001). “Experiments in Microbiology”. Plant pathology and Biotechnology.4th Edition. 102: 245-278.
EU (2004). Nickel sulphate risk assessment. Prepared by the Danish Environmental Protection Agency for the European Union.
Garbarino JR., Hayes H. and Roth D. (1995). Contaminants in the Mississippi River, U. S. Geological Survey Circular, Virginia, U. S. A. 1133.
Gunasekaran P., Muthukrishnan J. and Rajendran P. (2003). Microbes in Heavy Metal Remediation. Indian Journal of Experimental Biology, 41: 935-944.
Hamza U. D., Mohammed A. I. and Ibrahim S. (2009). Kinetics Of Biological Reduction of Chemical Oxygen Demand From Petroleum Refinery Wastewater. Researcher, Vol 1(2).
Holt J. G., Krieg N. R., Sneath P. H. A., Stanley J. T and Williams S. T (1994). “Bergey’s manual of determinative Bacteriology”. 9th Ed. Williams and Wilkins, pp: 71-561.
Jern WNG (2006). Industrial Wastewater Treatment. Singapore: Imperial College Press.
Konopka A., Zakharova T., Bischoff M., Oliver T., Nakastu. C and Turco R. F. (1990). Microbial biomass and activity in lead contaminated soil. Journal of Applied Environmental Microbiology, 65(5): 2256-2259.
Mgbema I. C., Nnokwe J. C., Adjeroh L. A. and Onyemekara N. N. (2012). Resistance Of Bacteria Isolated From Otamiri River To Heavy Metals And Some Selected Antibiotics. Current Research Journal Of Biological Sciences, 4(5): 551-556.
Momba N. B. and Kamika I. (2013). Assessing the Resistance And Bioremediation Ability Of Selected Bacterial And Protozoan Species To Heavy Metals In Metal-Rich Industrial Wastewater. BioMedical Central Microbiology. Pp 1-14.
Nies, D. H, (1999), Microbial Heavy Metal resistances. Applied Microbial biotechnology, 51: 730-750.
Ojo O A (2006). “Petroleum Hydrocarbon Utilization By Native Bacterial Population From A Wastewater Canal Southwest Nigeria”. African journal of Biotechnology, 5: 333-337.
Philip L, Iyengar L, Venkobacher L, 2000. Site of interaction of copper on Bacillus polymyxa. Water Air Soil Pollution 119: pp 1121.
Rajbanshi A. (2008). Study On Heavy Metal Resistance Bacteria In Guesswork Sewage Treatment Plant. Our Nature, 6: 52-57.
Sar P, Kazy SK, Asthana RK and Singh S. P, (1999). Metal adsorption and desorption by lyophilized Pseudomonas aeruginosa. International Biodeterrioration and Biodegradation, 44: 101-110.
Shi, W., Becker, J., Bischoff, M., Turco, R. F., and Konopka, A. E., (2002). Association of Microbial Community Composition And Activity With Lead, Chromium, And Hydrocarbon Contamination. Applied Environmental Microbiology, 68(8): 3859-3866.
Smrithi A. and Usha K. (2012). Isolation And Characterization Of Chromium Removing Bacteria From Tannery Effluent Disposal Site. International Journal Of Advanced Biotechnology And Research, 3(3): 644-652.
Usman D. H., Ibrahim A. M. and Abdullahi S. (2012). Potentials of Bacterial Isolates In Bioremediation Of Petroleum Refinery Wastewater. Journal of Applied Phytotechnology in Environmental Sanitation, 1(3): 131-138.