Bacterial Tolerance and Reduction of Chromium (VI) by Bacillus cereus Isolate PGBw4
American Journal of Environmental Protection
Volume 5, Issue 2, April 2016, Pages: 35-38
Received: Mar. 17, 2016; Accepted: Mar. 28, 2016; Published: Apr. 13, 2016
Views 3830      Downloads 144
Ferdouse Zaman Tanu, Dept. of Soil and Environmental Sciences, University of Barisal, Barisal, Bangladesh
Azizul Hakim, Dept. of Soil Science, University of Chittagong, Chittagong, Bangladesh
Sirajul Hoque, Dept. of Soil, Water and Environment, University of Dhaka, Dhaka, Bangladesh
Article Tools
Follow on us
This study aimed to determine the bacterial tolerance to chromium (Cr6+) in three growth media, such as nutrient broth, Luria Bertani (LB) broth and mineral salt media in terms of Minimum Inhibitory Concentration (MICs). Among the seven metal resistant soil bacteria, Bacillus cereus isolate PGBw4 and Bacillus cereus strain ES-4a1showed highest tolerance against Cr6+ in all three media. Bacillus cereus isolate PGBw4 was used as an effective and environment friendly agent for detoxifying Cr(VI) and reduction study in this research. The bacterial isolate mitigated toxic effects of Cr(VI) more efficiently from 100mg/L to 500mg/L within 24 and 48 hours respectively. The maximum amount of reduction of Chromium (VI) was 70.67 percent at 100 of Cr(VI) mg/L concentration after 48 hours of incubation and the lowest was 42 percent at 500mg/L Chromium concentration after 24 hours of incubation.
Chromium (VI), Bacillus cereus Isolate PGBw4, Tolerance, Reduction
To cite this article
Ferdouse Zaman Tanu, Azizul Hakim, Sirajul Hoque, Bacterial Tolerance and Reduction of Chromium (VI) by Bacillus cereus Isolate PGBw4, American Journal of Environmental Protection. Vol. 5, No. 2, 2016, pp. 35-38. doi: 10.11648/j.ajep.20160502.13
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Ren, W. X., P. J. Li, Y. Geng, and X. J. Li. 2009. Biological leaching of heavy metals from a contaminated soil by Aspergillus niger. J. Hazardous materials, 167(1-3), pp. 164-169.
Deeb, B. E. and A. D. Altalhi. 2009. Degradative plasmid and heavy metal resistance plasmid naturally coexist in phenol and cyanide assimilating bacteria. American J. Biochem. And Biotechnol., 5 (2), pp. 84-93.
Shen, H. and Wang, Y. 1995. Simultaneous chromium reduction and phenol degradation in a coculture of Escherichia coli ATCC 33456 and Pseudomonas putida DMP-1.Applied and Environmental Microbiol., 61, pp. 2754–2758.
Agrawal, A., V. Kumar and B. D. Pandy. 2006. Remediation Options for the Treatment of Electroplating and Leather Tanning Effluent Containing Chromium-a Review. Mineral Processing and Extractive Metallurgy Review, 27(2) pp. 99-130. doi: 10.1080/08827500600563319.
Benedict, R. G. and D. A. Carlson. 1971. Aerobic heterotrophic bacteria in activated sludge. Water Research, 5, pp. 1023–1030.
Bopp, L. H. and H. L. Ehrlich. 1988. Chromate resistance and reduction in Pseudomonas fluorescens strain LB300. Archives Microbiology 150, pp. 426–431.
Ishibashi, Y., C. Cervantes and S. Silver. 1990. Chromium reduction in Pseudomonas putida. Applied and Environmental Microbiol., 56, pp. 2268–2270.
Ahluwalia, S. S. and D. Goyal. 2007. Microbial and Plant Derived Biomass for Removal of Heavy Metals from Wastewater. Bioresource Tech., 98(12), pp. 2243-2257. doi:10.1016/j.biortech.2005.12.006.
Naja, G. and B. Volesky. 2006. Behavior of Mass Transfer Zone in a Biosorption Column. Environmental Science and Tech., 40(12), pp. 3996-4003. doi: 10.1021/es051542p.
Lovely, D. R. and E. J. P. Phillips. 1994. Reduction of Chromate by Desulfovibrio Vulgaris and Its C3 Cytochrome. Applied and Environment Microbiol., 60(2), pp. 726-728.
Tanu, F. Z. and S. Hoque. 2012. Bacterial tolerance to heavy metal contents present in contaminated and uncontaminated soils. Bangladesh J. Microbiol., 29(2), pp. 56-61.
Roane, T. M. and S. T. Kellogg. 1996. Characterization of bacterial communities in heavy metal contaminated soils. Can. J. Microbiol., 42, pp. 593-603.
Urone, P. F. 1955. Stability of colorimetric reagent for chromium, S-diphenylcarbazide in various solvents. Anal Chem., 27, pp. 1354–1355
Standard Methods for the Examination of Water and waste Waters. 1998. 20th edition. Washington, DC, USA: APHA (American Public Health Association),-AWWA-WEF.
Kumar R., M. Nongkhlaw, C. Acharya and S. R. Joshi. 2013. Growth media composition and heavy metal tolerance behavior of bacteria characterized from the sub-surface soil of uranium rich ore bearing site of Domiasiat in Meghalaya. Indian J. Biotechnol., 12, pp. 115-119.
Rajbanshi, A. 2008. Study on heavy metal resistant bacteria in Guheswori sewage treatment plant. Our Nature, 6, pp. 52–57.
Mahmood, S., A. Khalid, T. Mahmood, M. Arshad, and R. Ahmad. 2013. Potential of newly isolated bacterial strains for simultaneous removal of hexavalent chromium and reactive black-5 azo dye from tannery effluent. J. Chem. Technol. Biotechnol., 88, pp. 480-487.
McLean, J., T. J. Beveridge and D. Phipps. 2000. Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate contaminated site. Environ. Microbiol., 2, pp. 611-619.
DeLeo, P. C. and H. L. Ehrlich. 1994. Reduction of hexavalent chromium by Pseudomonas fluorescens LB300 in batch and continuous cultures. Appl. Microbiol. Biotechnol., 40, pp. 756-759.
Mclean, J. and T. J. Beveridge. 2001. Chromate reduction by a Pseudomonad isolated from a site contaminated with chromated copper arsenate. Appl. Environ. Microbiol., 67, pp. 1076-1084.
Sikander, S. and H. Shahida. 2007. Reduction of toxic hexavalent chromium by Ochrobactrumintermedium strain SDCr-5 stimulated by heavy metals. Bioresource Technol., 98, pp. 340-344.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186