Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae
Journal of Energy and Natural Resources
Volume 1, Issue 1, December 2012, Pages: 1-7
Received: Nov. 29, 2012; Published: Dec. 30, 2012
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Authors
Nalin Samarasinghe, Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA
Sandun Fernando, Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA
Brock Faulkner, Department of Biological and Agricultural Engineering, Scoates Hall, TAMU, Texas A&M University, College Station TX USA
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Abstract
The ability to extract lipids from high-moisture Nannochloris Oculata algal biomass disrupted with high pressure homogenization was investigated. During the first phase, the effect of high pressure homogenization (system pressure and number of passes) on disrupting aqueous algae (of different concentrations and degree of stress) was investigated. Se-condly, the effect of degree of cell wall disruption on the amount of lipids extracted with three solvents, namely: hexane, dichloromethane and chloroform, were compared. Studies reveled that high pressure homogenization is effective on cell disruption while the amount of system pressure being the most significant factor affecting the degree of cell breakage. Al-though the number of passes had some impact, the level of disruption seemed to level-off after a certain number of passes. The study revealed that slightly polar solvents (such as chloroform and dichloromethane) performed better in aqueous-phase lipid extractions as compared to hexane. Also, it was revealed that it was not necessary to disrupt the algal cells completely to achieve appreciable levels of lipid yields. In fact, conditions that exerted only 20% of the cells to completely disrupt, allowed sufficient damage to liberate most of the lipids contained in the remainder of the cells.
Keywords
Microalgae, Homogenization, Extraction, Lipids
To cite this article
Nalin Samarasinghe, Sandun Fernando, Brock Faulkner, Effect of High Pressure Homogenization on Aqueous Phase Solvent Extraction of Lipids from Nannochloris Oculata Microalgae, Journal of Energy and Natural Resources. Vol. 1, No. 1, 2012, pp. 1-7. doi: 10.11648/j.jenr.20120101.11
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