Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria
International Journal of Sustainable and Green Energy
Volume 4, Issue 2, March 2015, Pages: 40-46
Received: Jan. 29, 2015; Accepted: Feb. 8, 2015; Published: Mar. 30, 2015
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Authors
Usman Yahaya, Forestry Research Institute of Nigeria, Trial Afforestation Research Station, Kaduna, Nigeria
Umar Yahaya Abdullahi, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Denwe Samuel Dangmwan, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Muhammad Muktar Namadi, Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria
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Abstract
The economic and ecological problems associated with fossil fuel have raised interest in biofuel research in recent times in different parts of the world. The use of Eucalyptus forest waste biomass with no appreciable value to industries or for food as alternative and cost effective feedstock for bioethanol production was evaluated in this study. E. camaldulensis biomass (bark and leaves) were pretreated separately with acid (2M H2SO4) and Microwave irradiation (250V, 50Hz) prior to fermentation with Bacillus subtilis and Escherichia coli isolated from surrounding soil. Higher yield of reducing sugar were obtained from bark (43 %) and leaves (38.5 %) pretreated by microwave irradiation as compared with acid treated plant biomass. Similarly, Bioethanol volume and concentration of 34.89 g/l and 0.51 % respectively were higher in Microwave irradiated bark of E. camaldulensis at 21 days of fermentation when E. coli and B. subtilis were used in synergy The least bioethanol volume yield of 18.79 g/l and concentration of 0.12 % when bark and leaves of E. camaldulensis were combined was obtained on day 7 of fermentation using E. coli. The study concludes that the amount of dried wastes generated (37.8 kg) from one average stand of Eucalyptus tree could yield significant volume (131,884.2 g/l) of bioethanol when B. subtilis and E. coli are used in synergy.
Keywords
Eucalyptus, Biomass, Bioethanol, Fermentation
To cite this article
Usman Yahaya, Umar Yahaya Abdullahi, Denwe Samuel Dangmwan, Muhammad Muktar Namadi, Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria, International Journal of Sustainable and Green Energy. Vol. 4, No. 2, 2015, pp. 40-46. doi: 10.11648/j.ijrse.20150402.13
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