Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria
International Journal of Sustainable and Green Energy
Volume 4, Issue 2, March 2015, Pages: 40-46
Received: Jan. 29, 2015; Accepted: Feb. 8, 2015; Published: Mar. 30, 2015
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Usman Yahaya, Forestry Research Institute of Nigeria, Trial Afforestation Research Station, Kaduna, Nigeria
Umar Yahaya Abdullahi, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Denwe Samuel Dangmwan, Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Muhammad Muktar Namadi, Department of Chemistry, Nigerian Defence Academy, Kaduna, Nigeria
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The economic and ecological problems associated with fossil fuel have raised interest in biofuel research in recent times in different parts of the world. The use of Eucalyptus forest waste biomass with no appreciable value to industries or for food as alternative and cost effective feedstock for bioethanol production was evaluated in this study. E. camaldulensis biomass (bark and leaves) were pretreated separately with acid (2M H2SO4) and Microwave irradiation (250V, 50Hz) prior to fermentation with Bacillus subtilis and Escherichia coli isolated from surrounding soil. Higher yield of reducing sugar were obtained from bark (43 %) and leaves (38.5 %) pretreated by microwave irradiation as compared with acid treated plant biomass. Similarly, Bioethanol volume and concentration of 34.89 g/l and 0.51 % respectively were higher in Microwave irradiated bark of E. camaldulensis at 21 days of fermentation when E. coli and B. subtilis were used in synergy The least bioethanol volume yield of 18.79 g/l and concentration of 0.12 % when bark and leaves of E. camaldulensis were combined was obtained on day 7 of fermentation using E. coli. The study concludes that the amount of dried wastes generated (37.8 kg) from one average stand of Eucalyptus tree could yield significant volume (131,884.2 g/l) of bioethanol when B. subtilis and E. coli are used in synergy.
Eucalyptus, Biomass, Bioethanol, Fermentation
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Usman Yahaya, Umar Yahaya Abdullahi, Denwe Samuel Dangmwan, Muhammad Muktar Namadi, Bioethanol Production from Eucalyptus camaldulensis Wood Waste Using Bacillus subtilis and Escherichia coli Isolated from Soil in Afaka Forest Reserve, Kaduna State Nigeria, International Journal of Sustainable and Green Energy. Vol. 4, No. 2, 2015, pp. 40-46. doi: 10.11648/j.ijrse.20150402.13
G. Berndes, M. Hoogwijk and R.Van den Broek. “The contribution of biomass in the future global energy supply: a review of 17 studies”. Biomass and Bioenergy. vol. 25, pp. 1-28, 2003.
A. Kumar. “Next generation bio-fuels for greenhouse gas mitigation and role of biotechnology”. [Proc. Natl Conf. Emerging trends in biotechnology and pharmaceutical research, Mangalayatan University, Aligarh Feb. 18-19, pp 38, 2012].
A.M. Agba, M.E. Ushie, F.I. Abam, M.S. Agba and J. Okoro. “Developing the Biofuel Industry for Effective Rural Transformation”. European Journal of Scientific Research vol. 40, pp. 441-449, 2010.
C.V. Stevens and R. Verhe. Renewable bioresources scope and modification for nonfood applications. John Wiley and Sons Ltd, England, 2004, pp 310.
A.C. Hansen, Q. Zhang and P.W.L. Lyne. “Ethanol–diesel fuel blends: a review”. Bio resource Technology, vol. 96, pp. 277–285, 2005.
J. D. McMillan. “Bioethanol production: Status and prospects”. Renewable Energy, vol. 10(2), pp. 295-302, 1997.
Y. Zheng, Z. Pan, R. Zhang and D. Wang. “Overview of biomass pretreatment for cellulosic ethanol production”. International Journal of Agriculture and Biol Eng, vol. 2(3), pp. 51 – 68, 2009.
M. Galbe and G. Zacchi. “Pretreatment of lignocellulose materials for efficient bioethanol production”. Advances in Biochemical Engineering / Biotechnology, vol. 108, pp. 41-65, 2007.
A. Musliu and W. Salawudeen. “Screening and isolation of soil bacteria for ability to produce antibiotics”. European Journal of applied Science, vol. 4(5), pp. 211-215, 2012.
B.S Manga and S.B. Oyeleke. Essentials of Laboratory Practical’s in Microbiology 1st ed., Tobes Publishers, 2008, pp. 56-76.
B. Chitra, P. Harsha, G. Sadhana and R. Soni. “Isolation and characterization of bacterial isolates from agricultural soil at Durg district”. Indian journal of Science Research, vol. 4(1), pp. 221-226, 2014.
S. Nivedita, R. Shelly and P. Shruti. “Production, Purification and Characterization of cellulose free-xylanase by Bacillus coagulans B30 using lignocellulosic forest wastes with different pretreatment methods”. Journal of Agroalimentary processes and technologies, vol. 19(1), pp. 28-36, 2013.
A. Martinez, M.E. Rodriques, S.W. York, J.F. Preston and L.O. Ingram. “Effect of Ca(OH)2 treatments on the composition and toxicity of bagasse Hemicellulose Hydrolysates”. Biotechnology and Bioengineering, vol. 6, pp. 526-36, 2000.
G.L. Miller. “Use of dinitrosalicylic acid reagent for the determination of reagent for the determination of reducing sugar”. Analytical Chemistry, vol. 31(3), pp. 426-428, 1959.
S.B. Oyeleke and N.M. Jibrin. “Production of bioethanol from guinea corn husk and millet husk” .African journal of Microbiology Research, vol. 3(4), pp. 147-151, 2009.
C.N. Humphrey and U.O. Caritas. “Optimization of ethanol production from Garcinia kola (bitter kola) pulp agro waste”. African Journal of Biotechnology, vol. 6(17), pp. 2033-2037, 2007.
R. Arumugam and M. Manikandan. “Fermentation of pretreated hydrolyzates of banana and mango fruit wastes for ethanol production”. Asian Journal of Experimental Biological Science vol. 2(2), pp. 246-256, 2011.
M. Lima, B. Gabriela, K. Hana, B. Juliano, A. Camila, D. Oigres, R. Euduardo, D. Leonardo, J. Simon, A. Carlos and P. Igor. “Effect of pretreatment on morphology, chemical composition and enzymatic digestibility of Eucalyptus bark: a potentially valuable source of fermentable sugars for bioethanol production-part 1”. Biotechnology for biofuel, vol. 6, pp. 75, 2013.
V. Chaturvedi and P. Verma. “An overview of key pretreatment processes employed for bioconversion of lignocellulosic biomass into biofuels and value added products”. 3 Biotechnology, vol. 3(5), pp. 415-431, 2013.
M. Nikzad, K. Movagharnejad, G.D. Najafpour and F. Talebnia. “Comparative studies on effect of pretreatment of rice husk for enzymatic digestibility and bioethanol production”. International journal of engineering. Transactions B: Applications, vol. 26(5), pp. 455 – 464, 2013.
A.K. Mohd, S.K. Loh, A. Nasrin, A. Astimar and M.S. Rosnah. “Bioethanol production from empty fruit bunches hydrolysate using Saccharomyces cerevisiae”. Research Journal of Environmental Science, vol. 5(6), pp. 573-586, 2011.
j. Deutscher, A. Galinier and I. Martin-Verstraete. In: Carbohydrate uptake metabolism. Bacillus subtilis and its Closest Relatives: from Genes to Cells. A.L. Sonenshein, J.A. Hoch, R. Losick, Eds. American Society for Microbiology Press, Washington, DC, 2002, pp. 129–150.
A.A. Brooks. “Ethanol production potential of local yeast strains isolated from ripe banana peels”. African journal of Biotechnology, vol. 7(20), pp. 3749-3752, 2008.
B.S. Dien, M.A. Cotta and T.W. Jeffries. “Bacteria engineered for fuel ethanol production current Status”. Applied Microbiology and Biotechnology, vol. 63, pp. 258-266, 2003.
R.C. Dubey. A text book of Biotechnology, 4thed. Published in Ram Nagar, New Delhi, India, 2012, pp 545-547.
Z. Hu and Z. Wen.” Enhancing enzymatic digestibility of switch grass by microwave-assisted alkali pretreatment,” Biochemical Engineering Journal, vol. 38, pp. 369-378, 2008.
S.A. Nikolic. “Microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal”. Chemical Industry and Chemical Engineering, vol. 14(4), pp. 231−234, 2008.
A. Rabah, S. Oyeleke, S. Manga and L. Hassan. “Dilute acid pretreatment of millet and guinea corn husks for bioethanol production”. International Research Journal of Microbiology, vol. 2 (11), pp. 460-465, 2011.
R. Templer and R.J. Murphy. “Environmental sustainability of bioethanol production from waste paper: sensitivity to the system boundary”. Energy and Environmental Science, vol. 2, pp. 8281-8293, 2012.
J. Xiang, J. Ye, W.Z. Liang and P.M. Fan. “Influence of microwave on the ultrastructure of cellulose”. Journal of South China University of Technology, vol. 28, pp. 84-89, 2000.
Q. Yu, X. Zhuang, Z. Yuan, Q. Wang, W. Qi, W. Wang, Y. Zhang, J. Xu and H. Xu. “Two step liquid hot water pretreatment of Eucalyptus grandis to enhance sugar recovery and enzymatic digestibility”. Bioresource Technology, vol. 101, pp. 4895-4899, 2010.
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