Ethanolic Extracts of Vitex Doniana Possesses Hepatocuractive Property in Poloxamer Induced Hyperlipidemia
Science Research
Volume 2, Issue 3, June 2014, Pages: 49-54
Received: Apr. 25, 2014; Accepted: May 28, 2014; Published: Jun. 20, 2014
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Victor Duniya Sheneni, Department of Biochemistry, Ahmadu Bello University, Samaru Zaria, Nigeria
Dorcas Bolanle James, Department of Biochemistry, Ahmadu Bello University, Samaru Zaria, Nigeria
Sunday Eneojo Atawodi, Department of Biochemistry, Ahmadu Bello University, Samaru Zaria, Nigeria
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The hypolipidemic effect of Vitex doniana ethanolic leaves, stem bark and root bark extracts on some biochemical parameters in Poloxamer 407 (P407) induced hyperlipidemic rats was studied for a period of 21 days. Fifty four mixed sex rats weighing 100-200g were divided into nine groups comprising six animals per group: group given feed and water only, group induced by an intra-peritoneal injection of P407 every 48 hours without treatment, groups induced and treated with atorvastatin, leaves, stem bark, root bark extracts and groups of normal rats treated with leaves, stem bark and root bark extracts without induction. In all the groups, P407, atorvastatin, leaves, stem bark and root bark extracts were administered at a dose of 1000mg/kg, 20mg/kg, 100mg/kg, 100mg/kg and 30mg/kg body weight respectively. Blood samples were collected from the rats in all the groups at the end of the 21 days of treatment for determination of serum levels of; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB), indirect bilirubin (IB) and direct bilirubin (DB) using Randox kits. Serum concentration of liver marker enzymes (AST, ALT and ALP) was significantly (p<0.05) higher in hyperlipidemic control group when compared to normal control and all treated groups. However there was a significant (p<0.05) decrease and non-significant (p>0.05) change in the levels of these marker enzymes in hyperlipidemic groups treated with the extracts and normal treated groups when compared with the hyperlipidemic and normal control group respectively. There was a significantly (p<0.05) lower level of TP, DB and a significantly (p<0.05) higher level of TB, ID in hyperlipidemic group when compared with rats in the normal control and all treated groups. Normal groups treated with the extracts without induction shows a non-significant (p>0.05) difference in TP, DB, TB and ID when compared with normal control group. These results highlight the efficacy of Vitex doniana (leaves, stem bark and root bark) ethanolic extracts in the amelioration of some undesirable effects of hyperlipidemia.
Vitex Doniana, Hyperlipidemia, Poloxamer 407, Biochemical Parameters
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Victor Duniya Sheneni, Dorcas Bolanle James, Sunday Eneojo Atawodi, Ethanolic Extracts of Vitex Doniana Possesses Hepatocuractive Property in Poloxamer Induced Hyperlipidemia, Science Research. Vol. 2, No. 3, 2014, pp. 49-54. doi: 10.11648/
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