The Profile of the Expression of IBMR3 Antigens 3T3 and HT29 Cancer Cell Line after Analysis
Science Research
Volume 2, Issue 4, August 2014, Pages: 55-61
Received: Jun. 30, 2014; Accepted: Jul. 15, 2014; Published: Jul. 30, 2014
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Author
Qutaiba Kafi Jassim Alrawi, Laboratory Department, faculty of Medicine Technology of Surman, Alzawiah University, Surman, Libya
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Abstract
Aims: The aim of this study was to evaluate the effectiveness of specific monoclonal IBMR3 antibodies expression in the cancer cell lines of 3T3 and HT29, in order to recognize specific antigen and make evaluation, using immunoblotting technique which is widely use in this filed. Methods: Protein extracts were extracted from these cancer cell lines and used for western bloat. The Immunobloting were consequently subjected to densitometry analysis using bioimaging machine. This bioimaging process will facilitate to measure the molecular weights, peak height and raw volume of the protein bands for the 3T3 and HT29 cancer cell lines, which helps in diagnosis of any pathogenic antigen. Results: The bands obtained from bioimaging were exposed on the PVDF membrane. In the 3T3 bio imaging process revealed four bands and molecular weight were 299.58, 87.90, 41.67 and 23.54 KDa. However, bioimaging results for HT29 revealed also four bands with molecular weights: 90.11, 41.31, 23.87 and 20.86 KDa. The results of Peak height densitometry for IBMR3 antigen bands for 3T3 were: 1856.985, 551.769, 394.164, and 216.185. However HT29 peak height results were: 281.544, 101.711.202.668, 757.213. The raw volume (amount of protein bands of IBMR3 Ag for 3T3) were: 1460168.75, 206078.47, 161406.89, and 219583.16. However HT29 raw volume results were: 110197.11, 76106.84, 98632.59, and 221395.34. Negative protein staining for 3T3 and HT29 cancer cell line bands were done by using mouse IgM serotype. IgM serotype was not indicated that means no specific antigens for negative control IgM. Conclusion: The bioimaging revealed different results of the expression profile in molecular weight, peak height and raw volume between the two cancer cell lines. The results from this study suggest that the IBMR3 antigens were differentially expressed in 3T3 and HT29 and the molecular weight is higher in the 3T3 than HT29 cancer cell lines. In future it will be beneficial to categorize and study the character analysis of the IBMR3 antigen and its prospective role in cancer cells.
Keywords
3T3 (Mouse Fibroblast) Cell Line and HT29 (Human Colorectal) Carcinoma Cancer Cell Line, SDS-Page,Monocloncal Antibodies, MAb IBMR3, Immunobloting, Bioimaging
To cite this article
Qutaiba Kafi Jassim Alrawi, The Profile of the Expression of IBMR3 Antigens 3T3 and HT29 Cancer Cell Line after Analysis, Science Research. Vol. 2, No. 4, 2014, pp. 55-61. doi: 10.11648/j.sr.20140204.11
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Abbreviations: mAb (monoclonal anti body), HRP (horse radish peroxidase), PVDF (Polyvinylidene difluoride membrane), IgG (Immunoglobulin G), IgM (Immunoglobulin M), (IgS Immunoglobulin S). IL-4 (Human Interleukin – 4. recapture)
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