HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration
Science Research
Volume 3, Issue 3, June 2015, Pages: 89-92
Received: Feb. 21, 2015; Accepted: Mar. 1, 2015; Published: May 28, 2015
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Authors
Bushra Munir, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Bilal Ahmed, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Abdul Ghaffar, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Tahira Iqbal, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Naila Rafiq, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
Farah Latif, Department of Applied chemistry and Biochemistry, Govt. College University Faisalabad, Faisalabad, Pakistan
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Abstract
Pyrazinamide is use for the treatment of tuberculosis in all over the world. Bio kinetics deals with the mathematical description of drug changes in the body with time function. This study was designed to investigate the bio kinetics of Pyrazinamide after oral administration of Pyrazinamide (25mg) tablet in 10 healthy volunteers. The blood samples of each volunteer were collected from 0.5 to 12 hours at different time intervals after the medication. The concentration of Pyrazinamide in plasma samples were determined by high performance liquid chromatography (HPLC). Quantitative observations were recorded that include the mean ± SD value of absorption rate constant (Ka), time to peak (Tmax) and peak concentration (Cmax) were 0.38 ± 0.25/h, 2.248 ± 0.64 h and 4.165 ± 2.13µg/mL, respectively. The mean ± SD values of absorption half-life (t1/2a) and elimination half-life (t1/2b) were 2.078 ± 1.678h and 0.2908 ± 1.721 hours. The mean ± SD values of volume of distribution and total body clearance were 40 ± 27.4L and 3.351 ± 1.27 h, respectively. Mean residue time (MRT) showed mean ± SD and area under curve (AUC) were 17.23 ± 12.78 h and 90.4 ± 76.2 h.mg/L, respectively.
Keywords
Tuberculosis, HPLC, Bio Kinetics, Pyrazinamide
To cite this article
Bushra Munir, Bilal Ahmed, Abdul Ghaffar, Tahira Iqbal, Naila Rafiq, Farah Latif, HPLC Analysis and Bio-kinetics Study of Pyrazinamide in Healthy Volunteers After Oral Administration, Science Research. Vol. 3, No. 3, 2015, pp. 89-92. doi: 10.11648/j.sr.20150303.17
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